Fan ZaiFeng

Potyviruses recruit host eIF4A3 to block m6A-mediated RNA decay by steric hindrance of viral RNA methylation in plants

Dezhi Peng, Laihua Dong, Pei Wang, Lianyi Zang et autres

N 6-methyladenosine (m6A), a critical epitranscriptomic modification, regulates RNA metabolism and antiviral defenses. However, how pathogens evade m6A-mediated RNA decay in plants remains poorly understood. Here, we uncover a dynamic m6A modification arms race during infection of sugarcane mosaic virus (SCMV), a …

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Chloroplast Fibrillin‐Mediated α‐Tocopherol Biosynthesis Impaired by a Virus to Enhance Infection and to Improve Drought Tolerance

Sijia Liu, Xuedong Liu, Xi Chen, Lianyi Zang et autres

Plastoglobules (PGs), the lipid droplets mainly within chloroplasts, are crucial for plant redox homeostasis and environmental adaptation. However, the regulation of PG under stress remains elusive. This study uncovers that PGs are targeted by the p22 protein encoded by an emerging virus-tomato …

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Rapid Detection of Banana Streak Virus by Loop‐mediated Isothermal Amplification Assay in South China

Jun Peng, Fan ZaiFeng, Junsheng Huang

Abstract Banana streak virus (BSV) is a significant constraint to banana production and genetic improvement. It is necessary to develop and use BSV detection strategies that are both reliable and sensitive for the management of the virus. A loop‐mediated isothermal amplification (LAMP) …

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Molecular identification of viral pathogens inducing Rehmannia glutinosa mosaic disease in Beijing.

Zhou Ying, Rui Zhang, Guo Song, Huai Xiao et autres

Prokaryotic expression of coat protein of Cherry virus A isolate Beijing and preparation of the polyclonal antiserum

Guo Song, Fu-Xing Zhang, Huai Xiao, Ying Zhou et autres

According to previous work,Cherry virus A Beijing isolate(CVA-BJ) cp gene was cloned into pET-28a vector and fusion protein was expressed in Escherichia coli strain BL21(DE3).After purification through affinity Ni column,the fusion protein was used to immunize the white rabbit to produce antiserum.The …

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Cloning, prokaryotic expression of the coat protein gene of Apple stem grooving virus and antiserum preparation.

Huai Xiao, Ying Zhou, Rui Zhang, Fan ZaiFeng et autres

Prokaryotic expression and subcellular immuno-localization in maize of the VPg protein of Sugarcane mosaic virus.

Yanhong Qin, Dongmei Liu, Zhou Tao, Fan ZaiFeng

The VPg gene of Sugarcane mosaic virus Beijing isolate(SCMV-BJ) was amplified by RT-PCR from maize(Zea mays) leaves infected by SCMV-BJ,and cloned into expression vector pET-28a.The recombinant plasmid was transformed into E.coli BL21(DE3)and induced by IPTG to express fusion protein.The result of SDS-PAGE …

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Immuno-localization of the CI protein of Sugarcane mosaic virus in the phloem cells of maize.

Yanhong Qin, Dongmei Liu, Zhou Tao, Fan ZaiFeng

Sequence analysis of the genes of two isolates of Grapevine leafroll-associated viruses from Liaoning

Meng Wang, Fei Fei, Tao Zhou, Cheng Yuqin et autres

The dormant cutting of grapevine(Vitis vinifera cv.Venus Seedless) with typical leafroll symptom originated from Xingcheng region in Liaoning Province was detected for Grapevine leafroll-associated viruses(GLRaVs) by RT-PCR.The results showed that two complete sequences of major coat protein(CP) gene of GLRaV-2(GenBank accession number …

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Maize dwarf mosaic disease.

Xifeng Wang, Fan ZaiFeng, G. P. Rao, Claude Bragard et autres

Pennisetum mosaic virus.

Fan ZaiFeng, Huaifang Li, G. P. Rao, Claude Bragard et autres

Molecular characterization of Rice black-streaked dwarf virus isolates causing maize rough dwarf disease in Shandong.

Chen Jia, Qin-Qin Zhu, Yuan CongYang, Zuowen Sun et autres

Four isolates of Rice black-streaked dwarf virus(RBSDV)were collected from the maize plants showing rough dwarf symptom in Linyi and Tai'an,Shandong province.The S10 genomic sequences of these isolates were determined and compared with those of 14 other RBSDV isolates.All of the four sequences …

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