Xiaolu Zhang

Comprehensive Landscape of Post-Translational Modification Alterations in Nephrolithiasis Revealing Activation of Multiple Cell Death Pathways

Yining Dong, Mengya Zhang, Xu Yang, Jianchao Zhang et autres

Nephrolithiasis is a common urinary disorder characterized by high prevalence and recurrence, but the molecular mechanisms underlying calcium oxalate (CaOx)-crystal-induced renal injury remain incompletely understood. We applied integrated proteomic, phosphoproteomic, acetylomic, and lactylomic analyses to kidney tissues from a mouse model of …

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CD48 + tumour-associated macrophages: novel immunotherapeutic targets in hepatocellular carcinoma

Xiaolu Zhang, Natascha Roehlen

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CDK11 Promotes Paclitaxel Resistance in Cervical Cancer by Regulating LATS1 ‐Mediated Hippo Signaling Pathway Through Phosphorylation of NF2

Yan Zhang, Jingyan Lu, Minfang Qi, Xiaolu Zhang et autres

Paclitaxel (PTX) is a first-line chemotherapeutic agent for cervical cancer, but resistance remains a major clinical challenge. This study investigates the role of cyclin-dependent kinase 11 (CDK11) in mediating PTX resistance and explores its underlying molecular mechanisms. Cell viability, proliferation, and apoptosis …

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Supplementary Tables from Hypoxia-Induced Creatine Uptake Reprograms Metabolism to Antagonize PARP1-Mediated Cell Death and Facilitate Tumor Progression in Hepatocellular Carcinoma

Rui‐Zhe Li, Guo‐Qiang Pan, Chen Xiong, Zi‐Niu Ding et autres

Supplementary Tables S1-S6

Supplementary Figure 2 from Hypoxia-Induced Creatine Uptake Reprograms Metabolism to Antagonize PARP1-Mediated Cell Death and Facilitate Tumor Progression in Hepatocellular Carcinoma

Rui‐Zhe Li, Guo‐Qiang Pan, Chen Xiong, Zi‐Niu Ding et autres

Supplementary Figure 2. Creatine promotes HCC progression and parthanatos antagonism under hypoxia. (A-B) Cell proliferation and cell death of HCC control and SLC6A8-knockdown cells treated with MNNG were assessed by CCK-8 (A) and LDH leakage (B), respectively. (C) qRT-PCR was used to …

Supplementary Figure 7 from Hypoxia-Induced Creatine Uptake Reprograms Metabolism to Antagonize PARP1-Mediated Cell Death and Facilitate Tumor Progression in Hepatocellular Carcinoma

Rui‐Zhe Li, Guo‐Qiang Pan, Chen Xiong, Zi‐Niu Ding et autres

Supplementary Figure 7. SERPINE1 reverses the attenuated deubiquitination activity of USP10 caused by the direct binding of PAR. (A) Western blot analysis of PKLR levels in USP10-knockdown HCC cells transfected with SERPINE1 plasmids. (B) Western blot analysis of PKLR levels in SERPINE1-knockdown …

Data from Hypoxia-Induced Creatine Uptake Reprograms Metabolism to Antagonize PARP1-Mediated Cell Death and Facilitate Tumor Progression in Hepatocellular Carcinoma

Rui‐Zhe Li, Guo‐Qiang Pan, Chen Xiong, Zi‐Niu Ding et autres

Abstract Recently, a PARP1-dependent cell death process termed “parthanatos” that is driven by DNA damage has emerged as a crucial regulator of tissue homeostasis and tumorigenesis. Hypoxia is a hallmark of solid tumors and profoundly affects the malignant phenotypes of cancer cells. …

Supplementary Figure 5 from Hypoxia-Induced Creatine Uptake Reprograms Metabolism to Antagonize PARP1-Mediated Cell Death and Facilitate Tumor Progression in Hepatocellular Carcinoma

Rui‐Zhe Li, Guo‐Qiang Pan, Chen Xiong, Zi‐Niu Ding et autres

Supplementary Figure 5. The oncogenic role of creatine depends on SERPINE1 to form a creatine/SERPINE1/HIF-1α positive feedback loop. (A-C) CCK-8 (A), EdU incorporation (B) and colony formation assays (C) were used to determine the role of SERPINE1 in SLC6A8-knockdown cell proliferation and …

Supplementary Figure 4 from Hypoxia-Induced Creatine Uptake Reprograms Metabolism to Antagonize PARP1-Mediated Cell Death and Facilitate Tumor Progression in Hepatocellular Carcinoma

Rui‐Zhe Li, Guo‐Qiang Pan, Chen Xiong, Zi‐Niu Ding et autres

Supplementary Figure 4. Creatine-mediated SERPINE1 upregulation inhibits parthanatos and aggravates HCC progression. (A) Western blot showing the regulatory effects of hypoxia and creatine/SLC6A8 on SERPINE1 expression. (B-D) CCK-8 (B), EdU incorporation (C) and colony formation assays (D) were used to determine the …

Supplementary Figure 8 from Hypoxia-Induced Creatine Uptake Reprograms Metabolism to Antagonize PARP1-Mediated Cell Death and Facilitate Tumor Progression in Hepatocellular Carcinoma

Rui‐Zhe Li, Guo‐Qiang Pan, Chen Xiong, Zi‐Niu Ding et autres

Supplementary Figure 8. Combining RGX-202 with lenvatinib exhibits potent antitumor effects in PDX models. (A, B) Colony formation assay was used to determine the colony formation ability of hypoxic HCC cells across different treatment groups. (C-F) Cell invasion and migration of hypoxic …

Supplementary Figure 1 from Hypoxia-Induced Creatine Uptake Reprograms Metabolism to Antagonize PARP1-Mediated Cell Death and Facilitate Tumor Progression in Hepatocellular Carcinoma

Rui‐Zhe Li, Guo‐Qiang Pan, Chen Xiong, Zi‐Niu Ding et autres

Supplementary Figure 1. Hypoxia-induced upregulation of SLC6A8 highly associates with parthanatos antagonism and tumor progression in HCC. (A-C) HCC cells were treated as described in Figure 1D, and immunofluorescence (A) and Western blotting (B, C) showed the nuclear translocation of AIF. Scale …

Supplementary Figure 3 from Hypoxia-Induced Creatine Uptake Reprograms Metabolism to Antagonize PARP1-Mediated Cell Death and Facilitate Tumor Progression in Hepatocellular Carcinoma

Rui‐Zhe Li, Guo‐Qiang Pan, Chen Xiong, Zi‐Niu Ding et autres

Supplementary Figure 3. Creatine antagonizes parthanatos by enhancing the metabolic reprogramming of hypoxic HCC cells. (A) Heatmap of the DEGs in hypoxic Huh7 SLC6A8-knockdown cells compared with control cells. (B-C) KEGG and GO analyses of Huh7 RNA-seq data. (D) Gene set enrichment …