Levi B. Gifford

Stalling the Enemy: Targeting Nsp13 for Next-Generation SARS-CoV-2 Antivirals

J. M. Castro, Ryan L. Slack, Yee Tsuey Ong, Hu Zhang et autres

The SARS-CoV-2 public health challenges have highlighted the urgent need for coronavirus-targeting life-saving therapeutics. Given the emergence of drug-resistant strains, the development of antivirals against viral proteins beyond the commonly targeted main protease or RNA-dependent RNA polymerase is critical. The SARS-CoV-2 nonstructural …

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Tetrapyrrole biosynthetic intermediates act as chloroplast‐to‐nucleus retrograde signals to regulate intercellular trafficking via plasmodesmata

Mohammad F. Azim, Levi B. Gifford, Mazen Alazem, Jesse D. Woodson et autres

Intercellular communication via plasmodesmata (PD) is essential for plant growth, development, and defense, yet its regulation remains poorly understood. We set out to identify the chloroplast retrograde signals that regulate intercellular trafficking via PD. Using a combination of Arabidopsis thaliana mutants and …

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TDP-43 directly inhibits mRNA accumulation in neurites through modulation of mRNA stability

Bryan B. Guzmán, Levi B. Gifford, Daniel Dominguez, Gurprit Bhardwaj et autres

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TDP-43 directly inhibits mRNA accumulation in neurites through modulation of mRNA stability

Charlie Moffatt, Ankita Arora, Katherine F. Vaeth, Bryan B. Guzmán et autres

The subcellular localization of many mRNAs to neuronal projections allows neurons to efficiently and rapidly react to spatially restricted external cues. However, for most of these RNAs, the mechanisms that govern their localization are unknown. Here, using subcellular fractionation and single-molecule RNA …

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The RNA-binding protein HNRNPA2B1 regulates neurite RNA abundance and motor-dependent cargo transport

Joelle Lo, Levi B. Gifford, Katherine F. Vaeth, Amber Baldwin et autres

RNA molecules are localized to subcellular regions through interactions between localization-regulatory cis-elements and trans-acting RNA binding proteins (RBPs). However, the identities of RNAs whose localization is regulated by a specific RBP, as well as the impacts of that RNA localization on cell …

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Heme acts a chloroplast-to-nucleus retrograde signal to regulate intercellular trafficking via plasmodesmata

Mohammad F. Azim, Levi B. Gifford, Jesse D. Woodson, Tessa M. Burch‐Smith

ABSTRACT Intercellular communication via plasmodesmata (PD) is essential for plant growth, development, and defense, yet its regulation remains poorly understood. Chloroplasts communicate information about the environment and the physiological state of the plant cell to the nucleus. It has been proposed that …

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TDP-43 directly inhibits RNA accumulation in neurites through modulation of RNA stability

Charlie Moffatt, Ankita Arora, Katherine F. Vaeth, Bryan B. Guzmán et autres

ABSTRACT The subcellular localization of hundreds of RNAs to neuronal projections allows neurons to efficiently and rapidly react to spatially restricted external cues. However, for the vast majority of these RNAs, the mechanisms that govern their localization are unknown. Here we demonstrate …

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HIV-1 Capsid Uncoating Is a Multistep Process That Proceeds through Defect Formation Followed by Disassembly of the Capsid Lattice

Levi B. Gifford, Gregory B. Melikyan

High Resolution Image Download MS PowerPoint Slide The HIV-1 core consists of a cone-shaped capsid shell made of capsid protein (CA) hexamers and pentamers encapsulating the viral genome. HIV-1 capsid disassembly, referred to as uncoating, is important for productive infection; however, the …

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Bioorthogonal click labeling of an amber-free HIV-1 provirus for in-virus single molecule imaging

Yuanyun Ao, Jonathan R. Grover, Levi B. Gifford, Yang Han et autres

Structural dynamics of human immunodeficiency virus 1 (HIV-1) envelope (Env) glycoprotein mediate cell entry and facilitate immune evasion. Single-molecule FRET using peptides for Env labeling revealed structural dynamics of Env, but peptide use risks potential effects on structural integrity/dynamics. While incorporating noncanonical …

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Label-free imaging of nuclear membrane for analysis of nuclear import of viral complexes

Andrew Ten Eyck, Yen-Cheng Chen, Levi B. Gifford, Dariana Torres-Rivera et autres

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Human Immunodeficiency Virus 1 Capsid Uncoating in the Nucleus Progresses Through Defect Formation in the Capsid Lattice

Levi B. Gifford, Gregory B. Melikyan

Abstract The HIV-1 core consists of a cone-shaped capsid shell made of ∼250 capsid protein (CA) hexamers and 12 pentamers encapsulating the viral genome. HIV-1 capsid disassembly, referred to as uncoating, is a highly regulated process that is important for productive infection, …

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