Accès ouvert
2023
article
OpenAlex
Abhi Aggarwal, Rui Liu, Yang Chen, Amelia J Ralowicz et autres
The fluorescent glutamate indicator iGluSnFR enables imaging of neurotransmission with genetic and molecular specificity. However, existing iGluSnFR variants exhibit low in vivo signal-to-noise ratios, saturating activation kinetics and exclusion from postsynaptic densities. Using a multiassay screen in bacteria, soluble protein and cultured …
us, de, cz, ch
(code pays fourni par la source)
Accès ouvert
2022
article
OpenAlex
Peter H. Chipman, Richard Doty Fetter, Lauren C. Panzera, Samuel J Bergerson et autres
Homeostatic plasticity (HP) encompasses a suite of compensatory physiological processes that counteract neuronal perturbations, enabling brain resilience. Currently, we lack a complete description of the homeostatic processes that operate within the mammalian brain. Here, we demonstrate that acute, partial AMPAR-specific antagonism induces …
us
(code pays fourni par la source)
Accès ouvert
2022
preprint
OpenAlex
Abhi Aggarwal, Rui Liu, Yang Chen, Amelia J Ralowicz et autres
Abstract The fluorescent glutamate indicator iGluSnFR enables imaging of neurotransmission with genetic and molecular specificity. However, existing iGluSnFR variants exhibit saturating activation kinetics and are excluded from post-synaptic densities, limiting their ability to distinguish synaptic from extrasynaptic glutamate. Using a multi-assay screen …
us, de, ch
(code pays fourni par la source)