LingSze Lee

Combining ultrastructure expansion microscopy with immunofluorescence and Oligopaint DNA FISH

Lorielle M. Raab, Clio B. Hockens, LingSze Lee, Rose K. Runyan et autres

Expansion microscopy (ExM) enlarges biological samples by embedding them in a swellable hydrogel, enabling nanoscale imaging of subcellular structures with standard light microscopes. This offers an accessible alternative to super-resolution methods. ExM has yet to be combined with the Oligopaint DNA fluorescence …

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Programmed meiotic errors facilitate dichotomous sperm production in the silkworm, Bombyx mori

Leif Benner, Makenzie Richmond, Youbin Xiang, LingSze Lee et autres

The goal of meiosis is typically to produce haploid gametes (eggs or sperm). Failure to do so is catastrophic for fertility. However, Lepidopteran (moths and butterflies) males produce two sperm morphs: nucleated (eupyrene) sperm and anucleated (apyrene) sperm, both of which are …

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Visualizing Drosophila centrioles by expansion microscopy

Anastasia Amoiroglou, Carey J. Fagerstrom, John M. Ryniawec, LingSze Lee et autres

A significant challenge in studying the biology of the Drosophila centriole is its small size. Advanced super-resolution techniques have provided valuable insights but require specialized equipment and can be difficult to implement in tissues. Expansion microscopy (ExM) offers an accessible alternative, yet …

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De novo genome assemblies of two cryptodiran turtles with ZZ/ZW and XX/XY sex chromosomes provide insights into patterns of genome reshuffling and uncover novel 3D genome folding in amniotes

Basanta Bista, Laura González-Rodelas, Lucía Álvarez-González, Zhiqiang Wu et autres

Understanding the evolution of chromatin conformation among species is fundamental to elucidate the architecture and plasticity of genomes. Nonrandom interactions of linearly distant loci regulate gene function in species-specific patterns, affecting genome function, evolution, and, ultimately, speciation. Yet, data from nonmodel organisms …

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Uncharted territories: Solving the mysteries of male meiosis in flies

LingSze Lee, Leah F. Rosin

The segregation of homologous chromosomes during meiosis typically requires tight end-to-end chromosome pairing. However, in Drosophila spermatogenesis, male flies segregate their chromosomes without classic synaptonemal complex formation and without recombination, instead compartmentalizing homologs into subnuclear domains known as chromosome territories (CTs). How …

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Evolution and dosage compensation of nucleolar organizing regions (NORs) mediated by mobile elements in turtles with female (ZZ/ZW) but not with male (XX/XY) heterogamety

Eugenia E. Montiel, Daleen Badenhorst, LingSze Lee, Nicole Valenzuela

Understanding the evolution and regulation of nucleolar organizing regions (NORs) is important to elucidate genome structure and function. This is because ribosomal gene (rDNA) copy number and activity mediate protein biosynthesis, stress response, ageing, disease, dosage compensation and genome stability. Here, we …

Evolution and dosage compensation of nucleolar organizing regions (NORs) mediated by mobile elements in turtles with female ( ZZ / ZW ) but not with male ( XX / XY ) heterogamety

Eugenia E. Montiel, Daleen Badenhorst, LingSze Lee, Nicole Valenzuela

Understanding the evolution and regulation of nucleolar organizing regions (NORs) is important to elucidate genome structure and function. This is because ribosomal gene (rDNA) copy number and activity mediate protein biosynthesis, stress response, ageing, disease, dosage compensation and genome stability. Here, we …

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Karyotypic Evolution of Sauropsid Vertebrates Illuminated by Optical and Physical Mapping of the Painted Turtle and Slider Turtle Genomes

LingSze Lee, Beatriz M. Navarro-Domínguez, Zhiqiang Wu, Eugenia E. Montiel et autres

Recent sequencing and software enhancements have advanced our understanding of the evolution of genomic structure and function, especially addressing novel evolutionary biology questions. Yet fragmentary turtle genome assemblies remain a challenge to fully decipher the genetic architecture of adaptive evolution. Here, we …

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Cytogenetics studies on the evolution of sex chromosomes and genome organization in turtles

LingSze Lee

Sex-determining mechanisms (SDMs) set an individual’s sexual fate by its genotype (genotypic sex determination, GSD) or environmental factors like temperature (temperature-dependent sex determination, TSD). While sexual reproduction is one of the most taxonomically conserved traits, SDMs are incredibly diverse and evolve frequently. …

Chromosomal Rearrangements during Turtle Evolution Altered the Synteny of Genes Involved in Vertebrate Sex Determination

LingSze Lee, Eugenia E. Montiel, Beatriz M. Navarro-Domínguez, Nicole Valenzuela

Sex-determining mechanisms (SDMs) set an individual's sexual fate by its genotype (genotypic sex determination, GSD) or environmental factors like temperature (temperature- dependent sex determination, TSD), as in turtles where the GSD "trigger" remains unknown. SDMs co-evolve with turtle chromosome number, perhaps because …

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Discovery of Putative XX/XY Male Heterogamety in Emydura subglobosa Turtles Exposes a Novel Trajectory of Sex Chromosome Evolution in Emydura

LingSze Lee, Eugenia E. Montiel, Nicole Valenzuela

The discovery of sex chromosome systems in non-model organisms has elicited growing recognition that sex chromosomes evolved via diverse paths that are not fully elucidated. Lineages with labile sex determination, such as turtles, hold critical cues, yet data are skewed toward hide-neck …

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Subject Index

Kazumi Matsubara, Yoshinori Kumazawa, Hidetoshi Ota, Chizuko Nishida et autres