Gertrud Wiedemann

Quantifying endothelial damage by digital droplet polymerase chain reaction (PCR) of endothelial cell-free DNA in COVID-19 patients

Tiphaine Ruggeri, Gertrud Wiedemann, Noëlia Schärz, Barbara Hügli et autres

Background: COVID-19, caused by SARS-CoV-2, triggers severe systemic inflammation and multiple organ dysfunction. Microvascular complications, potentially arising from endothelial cell infection and/or immunothrombosis, play a central role in the disease's pathophysiology. Upon cell activation and/or cell death, cells release cell-free DNA (cfDNA) …

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Quantifying endothelial damage by digital droplet polymerase chain reaction (PCR) of endothelial cell-free DNA in COVID-19 patients.

Tiphaine Ruggeri, Gertrud Wiedemann, Noëlia Schärz, Barbara Hügli et autres

Background COVID-19, caused by SARS-CoV-2, triggers severe systemic inflammation and multiple organ dysfunction. Microvascular complications, potentially arising from endothelial cell infection and/or immunothrombosis, play a central role in the disease's pathophysiology. Upon cell activation and/or cell death, cells release cell-free DNA (cfDNA) …

ru, de, gb (code pays fourni par la source)

Artificial Intelligence in Haematologic Diagnostics: Current Applications and Future Perspectives

Annatina Schnegg‐Kaufmann, Ulrike Bacher, Alicia Rovó, Martin Andres et autres

BACKGROUND: Clinical researchers and laboratory specialists are striving to explore artificial intelligence (AI) to facilitate and optimize haematological diagnostics in response to the growing demand for more efficient and accurate diagnoses. SUMMARY: This review summarizes current approaches integrating AI into blood and …

ch, Afrique du Sud (code pays fourni par la source)

Clonal Hematopoiesis and Outcomes After High-Dose Chemotherapy and Autologous Stem Cell Transplantation in Patients with AML, Myeloma, and Lymphoma

Katharina Sponagel, Ulrike Bacher, Katja Seipel, Naomi Porret et autres

Autologous stem cell transplantation (ASCT) after high-dose chemo-therapy (HDCT) is an option of consolidation therapy in patients with AML, lymphoma, or myeloma. Clonal hematopoiesis (CH) is a premalignant state, associated with an increased risk of hematological cancer. The incidence of CH in …

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A Comprehensive ddPCR Strategy for Sensitive and Reliable Monitoring of CAR-T Cell Kinetics in Clinical Applications

Gertrud Wiedemann, Ulrike Bacher, Raphael Joncourt, Françoise Solly et autres

In this study, we present the design, implementation, and successful use of digital droplet PCR (ddPCR) for the monitoring of chimeric antigen receptor T-cell (CAR-T) expansion in patients with B-cell malignancies treated with different CAR-T products at our clinical center. Initially, we …

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An ancient role for CYP73 monooxygenases in phenylpropanoid biosynthesis and embryophyte development

Samuel Knosp, Lucie Kriegshauser, Kanade Tatsumi, Ludivine Malherbe et autres

The phenylpropanoid pathway is one of the plant metabolic pathways most prominently linked to the transition to terrestrial life, but its evolution and early functions remain elusive. Here, we show that activity of the t-cinnamic acid 4-hydroxylase (C4H), the first plant-specific step …

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An ancient role for the CYP73 gene family in t -cinnamic acid 4-hydroxylation, phenylpropanoid biosynthesis and embryophyte development

Samuel Knosp, Lucie Kriegshauser, Kanade Tatsumi, Ludivine Malherbe et autres

ABSTRACT The phenylpropanoid pathway is a plant metabolism intimately linked to the transition to terrestrial life. It produces phenolic compounds that play essential roles in stress mitigation and ecological interactions. The pathway also provides the building blocks for hydrophobic polymers that form …

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Adaptive evolution of the enigmatic Takakia now facing climate change in Tibet

Ruoyang Hu, Xuedong Li, Yong Hu, Runjie Zhang et autres

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Correlation of Peripheral Chimeric Antigen Receptor T-cell (CAR-T Cell) mRNA Expression Levels with Toxicities and Outcomes in Patients with Diffuse Large B-cell Lymphoma

Christian Messerli, Gertrud Wiedemann, Naomi Porret, Michael Nagler et autres

Cytokine-release syndrome (CRS) and immune effector cell-associated neurotoxicity syndrome (ICANS) are significant complications in patients with relapsed/refractory diffuse large B-cell lymphoma undergoing chimeric antigen receptor T-cell (CAR-T cell) therapy. However, it remains unclear whether CAR-T cell expression itself is clinically relevant. We …

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Effect of MAPK activation via mutations in NRAS, KRAS and BRAF on clinical outcome in newly diagnosed multiple myeloma

Camille Perroud, Dario Thurian, Martin Andres, Arnaud Künzi et autres

Until now, next generation sequencing (NGS) data has not been incorporated into any prognostic stratification of multiple myeloma (MM) and no therapeutic considerations are based upon it. In this work, we correlated NGS data with (1) therapy response and survival parameters in …

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Real-life experiences with CAR T-cell therapy with idecabtagene vicleucel (ide-cel) for triple-class exposed relapsed/refractory multiple myeloma patients

Dilara Akhoundova, Katja Seipel, Ulrike Bacher, Marie-Noëlle Kronig et autres

BACKGROUND: Chimeric antigen receptor (CAR) T-cell therapy has revolutionized the treatment landscape of relapsed/refractory multiple myeloma (RRMM), leading to unprecedented responses in this patient population. Idecabtagene vicleucel (ide-cel) has been recently approved for treatment of triple-class exposed RRMM. We report real-life experiences …

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CAR T-Cell Persistence Correlates with Improved Outcome in Patients with B-Cell Lymphoma

Valerie Wittibschlager, Ulrike Bacher, Katja Seipel, Naomi Porret et autres

Chimeric antigen receptor (CAR) T-cell therapy has led to profound and durable tumor responses in a relevant subset of patients with relapsed/refractory (r/r) B-cell lymphomas. Still, some patients show insufficient benefit or relapse after CAR T-cell therapy. We performed a retrospective study …

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