Rafael Samaniego

“White and Clear” (WaC): A tunable hydrophilic tissue clearing strategy for multi‐species 3D imaging

Vicente Llorente, Elena Fernandez-Cortes, Daniel Sanderson, J Bueno Romero et autres

Abstract Optical tissue clearing (OTC) techniques have transformed three‐dimensional (3D) biological imaging, yet existing protocols often face limitations related to pigment persistence, reagent viscosity, or restricted applicability across tissues and species. Here, we introduce White and Clear (WaC), a tunable hydrophilic tissue …

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G-CSF associates with poor survival in cutaneous melanoma and promotes metastasis through coordinated effects on macrophages and tumor cells

Alicia Nieto‐Valle, Lucía Barandalla-Revilla, Baltasar López-Navarro, Celia Barrio‐Alonso et autres

ABSTRACT Macrophage-melanoma interactions critically shape the tumor microenvironment, yet the cytokine networks driving this process remain incompletely understood. Among these, colony-stimulating factors—particularly granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF or CSF3 )—regulate myeloid cell behavior during cancer progression, although their …

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Adverse prognosis of GM-CSF expression in human cutaneous melanoma

Elena García‐Martínez, Alicia Nieto‐Valle, Celia Barrio‐Alonso, Baltasar López-Navarro et autres

BACKGROUND: GM-CSF, a myeloid-priming cytokine, exhibits context-dependent effects on tumor growth and, despite its clinical use, its role in human melanoma remains undefined. METHODS: A stage II-IV primary melanoma cohort (n = 80) was interrogated for GM-CSF/GM-CSFR expression at the single-cell level …

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Adverse prognosis of GM-CSF expression in human cutaneous melanoma

Elena García‐Martínez, Alicia Nieto‐Valle, Celia Barrio‐Alonso, Baltasar López-Navarro et autres

Abstract Tumor-associated macrophages (TAMs) represent a major immune population within the tumor microenvironment, influencing cancer progression and immune responses. Our group previously identified a subset of pro-inflammatory TAMs associated with poor prognosis in human melanoma. GM-CSF, a myeloid-priming cytokine, exhibits context-dependent effects …

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Reprogramming of GM-CSF-dependent alveolar macrophages through GSK3 activity modulation

Israel Ríos, Cristina Herrero, Mónica Torres‐Torresano, Baltasar López-Navarro et autres

Monocyte-derived macrophages recruited into inflamed tissues can acquire an array of functional states depending on the extracellular environment. Since the anti-inflammatory/pro-fibrotic macrophage profile is determined by MAFB, whose activity/protein levels are regulated by GSK3, we addressed the macrophage reprogramming potential of GSK3 …

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Author response: Reprogramming of GM-CSF-dependent alveolar macrophages through GSK3 activity modulation

Israel Ríos, Cristina Herrero, Mónica Torres‐Torresano, Baltasar López-Navarro et autres

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Translating genetics into tissue: inflammatory cytokine-producing TAMs and PD-L1 tumor expression as poor prognosis factors in cutaneous melanoma

Celia Barrio‐Alonso, Alicia Nieto‐Valle, Lucía Barandalla-Revilla, José Antonio Avilés-Izquierdo et autres

Myeloid cells within tumor microenvironments exhibit significant heterogeneity and play a critical role in influencing clinical outcomes. In this study, we investigated the infiltration of various myeloid cell subtypes in a cohort of cutaneous melanomas, revealing no significant correlation between myeloid cell …

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Re-programming of GM-CSF-dependent alveolar macrophages through GSK3 activity modulation

Israel Ríos, Cristina Herrero, Mónica Torres‐Torresano, Baltasar López-Navarro et autres

Monocyte-derived macrophages recruited into inflamed tissues can acquire an array of functional states depending on the extracellular environment. Since the anti-inflammatory/pro-fibrotic macrophage profile is determined by MAFB, whose activity/protein levels are regulated by GSK3, we addressed the macrophage re-programming potential of GSK3 …

es (code pays fourni par la source)

Author response: Re-programming of GM-CSF-dependent alveolar macrophages through GSK3 activity modulation

Israel Ríos, Cristina Herrero, Mónica Torres‐Torresano, Baltasar López-Navarro et autres

Monocyte-derived macrophages recruited into inflamed tissues can acquire an array of functional states depending on the extracellular environment. Since the anti-inflammatory/pro-fibrotic macrophage profile is determined by MAFB, whose activity/protein levels are regulated by GSK3, we addressed the macrophage re-programming potential of GSK3 …

es (code pays fourni par la source)

Author response: Re-programming of GM-CSF-dependent alveolar macrophages through GSK3 activity modulation

Israel Ríos, Cristina Herrero, Mónica Torres‐Torresano, Baltasar López-Navarro et autres

Monocyte-derived macrophages recruited into inflamed tissues can acquire an array of functional states depending on the extracellular environment. Since the anti-inflammatory/pro-fibrotic macrophage profile is determined by MAFB, whose activity/protein levels are regulated by GSK3, we addressed the macrophage re-programming potential of GSK3 …

es (code pays fourni par la source)

Reprogramming of GM-CSF-dependent alveolar macrophages through GSK3 activity modulation

Israel Ríos, Cristina Herrero, Mónica Torres‐Torresano, Baltasar López-Navarro et autres

Monocyte-derived macrophages recruited into inflamed tissues can acquire an array of functional states depending on the extracellular environment. Since the anti-inflammatory/pro-fibrotic macrophage profile is determined by MAFB, whose activity/protein levels are regulated by GSK3, we addressed the macrophage reprogramming potential of GSK3 …

es (code pays fourni par la source)

Re-programming of GM-CSF-dependent alveolar macrophages through GSK3 activity modulation

Israel Ríos, Cristina Herrero, Mónica Torres‐Torresano, Baltasar López-Navarro et autres

Summary Monocyte-derived macrophages recruited into inflamed tissues can acquire an array of functional states depending on the extracellular environment. Since the anti-inflammatory/pro-fibrotic macrophage profile is determined by MAFB, whose activity/protein levels are regulated by GSK3, we addressed the macrophage re-programming potential of …

es (code pays fourni par la source)