Bangshun He

Cascade-Amplified Microfluidic Detection of Tumor Biomarkers via Metal-DNA Nanospheres Activating CRISPR-Cas12a Assay

Wenjian Zhao, Xiaowei Luan, Wenxiu Long, Zhi Li et autres

Abstract The development of ultrasensitive protein detection platforms is pivotal for early cancer diagnosis, yet existing methods are often constrained by inefficient “protein-to-signal” conversion and complex instrumentation. While CRISPR-Cas-based biosensors offer higher sensitivity for nucleic acids, their application in protein analysis is …

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Thermophoretic Nanomotors for Autophagic Turnover of HSP90 Potentiates Immunotherapy

Xiaohui Liu, Shurong Qin, Lu Wang, Wen Luo et autres

ABSTRACT Therapeutic barriers and resistance limit the efficacy of immunotherapy in metastatic breast cancer. Here, we report a dual‐nanobody autophagy‐targeting chimera (AUTAC), hereafter referred to as Nb, that selectively degrades heat shock protein 90 (HSP90) via p62‐dependent autophagy, restoring tumor sensitivity to …

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Getah virus non-structural protein 2 inhibits type I interferon production by preventing K63-linked polyubiquitination of IKKε and causing widespread cellular shutoff

Min Zhao, Likai Ji, Yi Zhao, Shixing Yang et autres

ABSTRACT Getah virus (GETV) is an emerging mosquito-borne alphavirus that poses a growing threat to animal and public health. However, the molecular mechanisms underlying its pathogenesis and immune evasion remain poorly defined. This study demonstrated that GETV infection suppressed the activation of …

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A Cascade Catalysis-Enhanced Microfluidic Platform for Rapid Foodborne Pathogen Detection Using Pt@Zr-MOF Nanozymes

Hui xue, Lingyun Wang, yong wang, Ying Xu et autres

Salmonella Typhimurium (S. typhimurium) has become a major pathogen in the field of food safety, seriously threatening human health. Although mainstream diagnostic assays, including culture-based method, enzyme-linked immunosorbent assay (ELISA),...

G-Quadruplexes Formation in the PAI-1 Gene Modulates Sepsis-Induced Coagulopathy and Mortality Risk

Fang Sun, Suhua Zhang, Zhenlin Nie, Bangshun He et autres

BackgroundEarly detection of sepsis-induced coagulopathy (SIC) is of great clinical significance, as its closely correlates with disease progression in patients with sepsis. In the present study, we focused on mining the major coagulation factors that participate in the development and progression of …

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Figure S2 from circADAMTS12 Inhibits the Antitumor Activity of Microglia to Enable Metastatic Colonization in the Brain

Zhengmiao Xia, Weiguang Liu, Danni Guo, Long Chen et autres

CircADAMTS12 circulation-dead mutant to facilitate the production of linear transcripts of circADAMTS12

Table S3 from circADAMTS12 Inhibits the Antitumor Activity of Microglia to Enable Metastatic Colonization in the Brain

Zhengmiao Xia, Weiguang Liu, Danni Guo, Long Chen et autres

CircRNA Transcription profiles of 231-BM cells vs 231-Par cells

Table S4 from circADAMTS12 Inhibits the Antitumor Activity of Microglia to Enable Metastatic Colonization in the Brain

Zhengmiao Xia, Weiguang Liu, Danni Guo, Long Chen et autres

Transcription profiles of 231-BM cells with vs without circADAMTS12 depletion

Table S2 from circADAMTS12 Inhibits the Antitumor Activity of Microglia to Enable Metastatic Colonization in the Brain

Zhengmiao Xia, Weiguang Liu, Danni Guo, Long Chen et autres

Primers for PCR and molecular cloning

Figure S4 from circADAMTS12 Inhibits the Antitumor Activity of Microglia to Enable Metastatic Colonization in the Brain

Zhengmiao Xia, Weiguang Liu, Danni Guo, Long Chen et autres

High expression of circADAMTS12 in tumor cells alters microglial inflammatory factor expression via conditioned medium

Figure S3 from circADAMTS12 Inhibits the Antitumor Activity of Microglia to Enable Metastatic Colonization in the Brain

Zhengmiao Xia, Weiguang Liu, Danni Guo, Long Chen et autres

CircADAMTS12 binds PARP1 and activates PARylation

Table S5 from circADAMTS12 Inhibits the Antitumor Activity of Microglia to Enable Metastatic Colonization in the Brain

Zhengmiao Xia, Weiguang Liu, Danni Guo, Long Chen et autres

List of enriched biological processes in circADAMTS12-depleted 231-BM cells as compared to 231-BM cells without circADAMTS12 depletion