Linda L. Burianek

Solvent extraction of bacteriocins from liquid cultures

Linda L. Burianek, Ahmed E. Yousef

A solvent extraction method was developed to concentrate lacidin from the culture of Lactobacillus acidophilus OSU133. The new method concentrates the bacteriocin at the interface between chloroform and the aqueous culture of the producing bacterium. Compared with other extraction procedures, the new …

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ASCORBIC ACID DEGRADATION KINETICS DURING CONVENTIONAL and OHMIC HIEATING

Marybeth Lima, BRIAN F. HESKITT, Linda L. Burianek, Sue E. Nokes et autres

Ascorbic acid (vitamin C) degradation during electrical and conventional heating was studied to determine if the presence of an electric field altered the rate of degradation. Experiments were peiformed using a static ohmic heating apparatus, and heating histories were matched using applied …

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Characterization of Mycobacterium paratuberculosis antigenic proteins

Steen Bech‐Nielsen, Linda L. Burianek, Lawrence E. Heider, Glen F. Hoffsis et autres

The characterization of a purified antigen from Mycobacterium paratuberculosis, recently made commercially available for use in serodiagnosis by enzyme-linked immunosorbent assay (ELISA), of paratuberculosis in cattle was described. This assay had 89% specificity and 83% sensitivity for M paratuberculosis infection. The protein/polypeptide …

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Interstrain mitochondrial DNA polymorphism detected in Acanthamoeba by restriction endonuclease analysis

Susana A. Bogler, C D Zarley, Linda L. Burianek, Paul A. Fuerst et autres

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Analysis of Mitochondrial DNA Variation as an Approach to Systematic Relationships in the Genus Acanthamoeba1

Thomas J. Byers, Susana A. Bogler, Linda L. Burianek

ABSTRACT Classification at the species level has been difficult in the genus Acanthamoeba. The taxonomic designations of a number of strains are in doubt and new approaches to classification are needed. We describe the use of electrophoretic patterns obtained with restriction enzyme …

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