Olga S. Fedorova

Mechanisms of search for specific sites in DNA by DNA-binding proteins

Maria V. Lukina, Olga S. Fedorova, Alexander A. Chernonosov, Vladimir V. Koval

Efficient recognition of specific sites in DNA by repair proteins requires resolution of the fundamental "speed-stability paradox." This paradox arises from the need to scan a vast genome rapidly while maintaining high accuracy in lesion detection. Classical models of protein-DNA interactions have …

ru (code pays fourni par la source)

Selective immobilization of fluorescent dye and photosensitizers on dumbbell like magnetite-gold nanoparticles for improved tumor theranostics

Iuliia V. Chudosai, Petr V. Ostroverkhov, Ekaterina A. Plotnikova, K. A. Stepanova et autres

ru, us (code pays fourni par la source)

Modern Approaches to Protein Engineering to Create Enzymes with New Catalytic Properties

Timofey E. Tyugashev, Olga S. Fedorova, Nikita A. Kuznetsov

Abstract Adenine-DNA-glycosylase MutY is a monofunctional enzyme and catalyzes hydrolysis of N-glycosidic bonds with adenine residues located opposite 8-oxonuanine residues in DNA. Rational design was carried out to construct mutant enzyme forms with altered catalytic activity. Structures of the MutY mutants were …

ru (code pays fourni par la source)

DNA Probes for Analysis of the Activity of Key Enzymes of the Base Excision DNA Repair Pathway in Human Cells

Irina V. Alekseeva, А. А. Кузнецова, Olga A. Kladova, Victoria О. Shender et autres

Abstract—The important role of DNA damage in the occurrence of various diseases, including cancer, has led to study of the mechanisms of genetic information stability, that have been carried out since the discovery of DNA repair systems. The question of the relationship …

ru (code pays fourni par la source)

Comparative Analysis of Family A DNA-Polymerases as a Searching Tool for Enzymes with New Properties

Anatoly A. Bulygin, А. А. Кузнецова, Olga S. Fedorova, Nikita A. Kuznetsov

Abstract—DNA polymerases catalyze DNA synthesis during DNA replication, repair, and recombination. A number of DNA polymerases, such as the Taq enzyme from Thermus aquaticus, are used in various applications of molecular biology and biotechnology, in particular as DNA amplification tools. However, the …

ru (code pays fourni par la source)

A protocol for capturing RNA-sensing innate immune receptors in multiple conformations by single-particle cryo-EM

Wenshuai Wang, Olga S. Fedorova, Anna Marie Pyle

Capturing different conformations of receptor proteins that are complexed with ligands by single-particle cryo-EM facilitates our understanding toward the mechanisms of ligand recognition and receptor activation cascades. Here, we present a protocol for capturing RNA-sensing innate immune receptors, such as RIG-I, in …

us (code pays fourni par la source)

DNA Probes for Analysis the Activity of Key Enzymes of the Base Excision DNA Repair Pathway in Human Cells

Irina V. Alekseeva, А. А. Кузнецова, Olga A. Kladova, Victoria О. Shender et autres

The important role of DNA damage in the occurrence of various diseases, including cancer, has led to study of the mechanisms of genetic information stability, that have been carried out since the discovery of DNA repair systems. The question of the relationship …

ru (code pays fourni par la source)

Comparative Analysis of DNA-Polymerases from Family A as a Tool to Search for Enzymes with New Properties

Anatoly A. Bulygin, А. А. Кузнецова, Olga S. Fedorova, Nikita A. Kuznetsov

DNA polymerases catalyze DNA synthesis during DNA replication, repair, and recombination. A number of DNA polymerases, such as the Taq enzyme from Thermus aquaticus, are used in various applications of molecular biology and biotechnology, in particular as DNA amplification tools. However, the …

ru (code pays fourni par la source)

Modern Approaches of Protein Engineering for the Creation of Enzymes with New Catalytic Properties

Timofey E. Tyugashev, Olga S. Fedorova, Nikita A. Kuznetsov

Adenine-DNA-glycosylase MutY is a monofunctional enzyme and catalyzes hydrolysis of N-glycosidic bonds with adenine residues located opposite 8-oxonuanine residues in DNA. Rational design was carried out to construct mutant enzyme forms with altered catalytic activity. Structures of the MutY mutants were calculated …

ru (code pays fourni par la source)

Fluorescently labeled human apurinic/apyrimidinic endonuclease APE1 reveals effects of DNA polymerase β on the APE1–DNA interaction

Artemiy S. Bakman, А. А. Кузнецова, Lyudmila V. Yanshole, Alexander A. Ishchenko et autres

ru, fr, kz (code pays fourni par la source)

Kinetic Features of 3′–5′–Exonuclease Activity of Apurinic/Apyrimidinic Endonuclease Apn2 from Saccharomyces cerevisiae

А. А. Кузнецова, Anastasia A. Gavrilova, Alexander A. Ishchenko, Murat K. Saparbaev et autres

cells, apurinic/apyrimidinic (AP) sites are primarily repaired by base excision repair. Base excision repair is initiated by one of two AP endonucleases: Apn1 or Apn2. AP endonucleases catalyze hydrolytic cleavage of the phosphodiester backbone on the 5' side of an AP site, …

ru, fr (code pays fourni par la source)

The Kinetic Mechanism of 3′-5′ Exonucleolytic Activity of AP Endonuclease Nfo from E. coli

Svetlana I. Senchurova, А. А. Кузнецова, Alexander A. Ishchenko, Murat K. Saparbaev et autres

apurinic/apyrimidinic (AP) endonuclease Nfo is one of the key participants in DNA repair. The principal biological role of this enzyme is the recognition and hydrolysis of AP sites, which arise in DNA either as a result of the spontaneous hydrolysis of an …

ru, fr (code pays fourni par la source)