Sanja Sviben

Shaping cryogenic 3D volume imaging by pFIB ion species and milling voltage

Sanja Sviben, Christopher Thompson, Corinna Braun, Krishnan Venkataraman et autres

Abstract Cryogenic plasma focused ion beam-scanning electron microscopy (cryo-pFIB-SEM) enables nanoscale volume imaging of vitrified cells and tissues, but the fidelity of each newly exposed block-face depends on how ions interact with compositionally heterogeneous biological material. Using xenon, argon, oxygen, and nitrogen …

us, nl (code pays fourni par la source)

Depth-resolved analysis reveals target-dependent cryo-FIB damage profiles

Robert Michael Nwokonko, M. Zuhaib Qayyum, Dimple Karia, Vishal Maingi et autres

Abstract Cryogenic focused ion beam (cryo-FIB) milling enables cellular cryo-electron tomography but can compromise high-resolution structural information near milled surfaces. Here we establish recombinant human apoferritin (apoF) as a reporter of depth-dependent structural preservation in cellular lamellae. ApoF yielded a 2.05 Å …

us, nl, jp (code pays fourni par la source)

Contextual High-Throughput 3D Volume Electron Microscopy Data Acquisition Using Artificial Intelligence

Tereza Konečná, Radek Jančík, Daniela Slamková, Bronislav Přibyl et autres

One of the greatest challenges when generating large and/or high-resolution three-dimensional (3D) volume electron microscopy (vEM) datasets is long acquisition times. We developed a method that leverages Artificial intelligence algorithms to increase acquisition throughput for 3D datasets by creating an AI-derived mask …

cz, us (code pays fourni par la source)

Contextual High-throughput 3D Volume Electron Microscopy Data Acquisition Using Artificial Intelligence

Hurník Konečná T, Radek Jančík, Daniela Slamková, Bronislav Přibyl et autres

Abstract One of the greatest challenges when generating large and/or high-resolution three-dimensional (3D) volume electron microscopy (vEM) datasets is long acquisition times. We developed a method that leverages Artificial Intelligence (AI) algorithms to increase acquisition throughput for 3D datasets by creating an …

us, il (code pays fourni par la source)

FIB-SEM Trypanosoma cruzi cell samples

Daniela Slamková, Hurník Konečná T, Sanja Sviben

EMPIAR, the Electron Microscopy Public Image Archive centered at EMBL-EBI, is a public resource for raw electron microscopy images related to EMDB, contains micrographs, particle sets and tilt-series.

FIB-SEM Mouse kidney tissue samples

D. Slamková, Hurník Konečná T, Sanja Sviben

Contextual High-Throughput 3D Volume Electron Microscopy Data Acquisition Using Artificial Intelligence

Hurník Konečná T, Radek Jančík, Daniela Slamková, Bronislav Přibyl et autres

us (code pays fourni par la source)

Modeling late-onset Alzheimer’s disease neuropathology via direct neuronal reprogramming

Zhao Qing Sun, Ji‐Sun Kwon, Yudong Ren, Shawei Chen et autres

Late-onset Alzheimer's disease (LOAD) is the most common form of Alzheimer's disease (AD). However, modeling sporadic LOAD that endogenously captures hallmark neuronal pathologies such as amyloid-β (Aβ) deposition, tau tangles, and neuronal loss remains an unmet need. We demonstrate that neurons generated …

us, gb, de (code pays fourni par la source)

Benthic macroinvertebrate communities change along a karstic barrage-lake system

Sanja Sviben, Renata Matoničkin Kepčija, Marina Vilenica, Ivančica Krulik et autres

Barrage-lake systems are a rare natural phenomenon with specific ecological characteristics that are greatly affected by tufa formation. Our main goal was to investigate benthic macroinvertebrate communities and their longitudinal variations within the lakes arranged in cascades in the Dinaric karst hydrosystem. …

hr (code pays fourni par la source)

Immuno-scanning electron microscopy of islet primary cilia

Sanja Sviben, Alexander J. Polino, Isabella Melena, Jing W. Hughes

The definitive demonstration of protein localization on primary cilia has been a challenge for cilia biologists. Primary cilia are solitary thread-like projections that have a specialized protein composition, but as the ciliary structure overlays the cell membrane and other cell parts, the …

us (code pays fourni par la source)

Immuno-Scanning Electron Microscopy of Islet Primary Cilia

Sanja Sviben, Alexander J. Polino, Isabella Melena, Jing W. Hughes

The definitive demonstration of protein localization on primary cilia has been a challenge for cilia biologists. Primary cilia are solitary thread-like projections that contain specialized protein composition, but as the ciliary structure overlays the cell membrane and other cell parts, the identity …

us (code pays fourni par la source)

Multiple Genes Core to ERAD, Macroautophagy and Lysosomal Degradation Pathways Participate in the Proteostasis Response in α1-Antitrypsin Deficiency

Jie Li, Francesca Moretti, Tunda Hidvegi, Sanja Sviben et autres

BACKGROUND & AIMS: In the classic form of α1-antitrypsin deficiency (ATD), the misfolded α1-antitrypsin Z (ATZ) variant accumulates in the endoplasmic reticulum (ER) of liver cells. A gain-of-function proteotoxic mechanism is responsible for chronic liver disease in a subgroup of homozygotes. Proteostatic …

us, ch, cn (code pays fourni par la source)