Jonathan B. Leano

Large-scale single-molecule analysis of tau proteoforms

James Joly, Vivekananda Budamagunta, Zhengjian Zhang, Brittany Nortman et autres

Proteins exist as diverse proteoforms resulting from a combination of genetic variation, alternative splicing and post-translational modifications. Current methods struggle to capture this complexity at the single-molecule level. Here we introduce Iterative Mapping of proteoforms, a method that enables massively parallel interrogation …

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Abstract 5305: Detection and quantification of proteins using protein identification by short-epitope mapping (PrISM)

Torri E. Rinker, Jarrett D. Egertson, Steven Kwang San Tan, Aisha Ellahi et autres

Abstract Introduction: Here, we demonstrate Protein Identification by Short-epitope Mapping (PrISM), which aims to provide comprehensive proteome analysis with broad dynamic range at single-molecule resolution by interrogating immobilized, intact proteins in parallel using multi-affinity probes. Improving the dynamic range and scale for …

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Abstract LB225: Toward comprehensive, single-molecule proteomics: Protein identification by short-epitope mapping

Jarrett D. Egertson, Cassandra Stawicki, Taryn E. Gillies, James H. Joly et autres

Abstract Protein Identification by Short-epitope Mapping (PrISM) is a novel proteomics method with the goal of comprehensive proteome quantification at single-molecule sensitivity. In this work, we present computational modeling demonstrating the feasibility of this goal and experimental application of the approach leveraging …

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A Cryptic TOG Domain with a Distinct Architecture Underlies CLASP-Dependent Bipolar Spindle Formation

Stephen L. Rogers, Kevin C. Slep, Jonathan B. Leano

CLASP is a key regulator of microtubule (MT) dynamics and bipolar mitotic spindle structure with CLASP mutants displaying a distinctive monopolar spindle phenotype. It has been postulated that cryptic TOG domains underlie CLASP’s ability regulate MT dynamics. Here, we report the crystal …

THE CLASP FAMILY REGULATES MICROTUBULE DYNAMICS BY USING AN ARRAY OF TOG-LIKE DOMAINS

Jonathan B. Leano

CLASP is a key regulator of microtubule (MT) dynamics and bipolar mitotic spindle formation, with mutants displaying chromosome aggregation, aberrant monopolar spindle morphologies, and aneuploidy. How CLASP binds the microtubule lattice to regulate MT dynamics and facilitate proper spindle assembly remains unknown; …

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Structures of TOG1 and TOG2 from the human microtubule dynamics regulator CLASP1

Jonathan B. Leano, Kevin C. Slep

Tubulin-binding TOG domains are found arrayed in a number of proteins that regulate microtubule dynamics. While much is known about the structure and function of TOG domains from the XMAP215 microtubule polymerase family, less in known about the TOG domain array found …

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Structures suggest a mechanism for energy coupling by a family of organic anion transporters

Jonathan B. Leano, Samir Batarni, Jacob Eriksen, Narinobu Juge et autres

Members of the solute carrier 17 (SLC17) family use divergent mechanisms to concentrate organic anions. Membrane potential drives uptake of the principal excitatory neurotransmitter glutamate into synaptic vesicles, whereas closely related proteins use proton cotransport to drive efflux from the lysosome. To …

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Structure and Mechanisms of an Anion Transporter Family

Robert M. Stroud, Jonathan B. Leano, Samir Batarni, Robert H. Edwards

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Structures of TOG1 and TOG2 From the Human Microtubule Dynamics Regulator CLASP1

Jonathan B. Leano, Kevin C. Slep

Abstract Tubulin-binding TOG domains are found arrayed in a number of proteins that regulate microtubule dynamics. While much is known about the structure and function of TOG domains in the XMAP215 microtubule polymerase family, less in known about the TOG domain array …

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E. coli D-galactonate:proton symporter in the inward open form

Jonathan B. Leano, Robert H. Edwards, Robert M. Stroud

A Cryptic TOG Domain with a Distinct Architecture Underlies CLASP-Dependent Bipolar Spindle Formation

Jonathan B. Leano, Stephen L. Rogers, Kevin C. Slep

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Bar codes and the clinical laboratory: adaptation perspectives.

William R. Dito, Seth McIntire, Jonathan B. Leano

This paper provides an overview of the functional capabilities of bar-code technology within the laboratory environment and assesses the problems and solutions encountered during its introduction in our laboratory. An idea of the potential costs and personnel savings is offered, demonstrating that …

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