Caroline A. Stockwell

High-Resolution 3D Bioprinted Hydrogel Scaffolds Enable Sustained Intraperitoneal Cell Delivery

Y J Zhang, Lauren E. Carlberg, Cali N. Colliver, Alain Valdivia et autres

Intraperitoneal (I.P.) delivery of cell-based therapeutics represents a promising strategy for treating regional peritoneal diseases; however, rapid cellular clearance severely limits therapeutic durability. A critical unmet need is the development of implantable biomaterial platforms that can both mechanically integrate within the dynamic …

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Abstract 660: Integrating passage-zero patient tumor tissues from distant institutions into a rapid, ex vivo drug screening platform.

Adebimpe Adefolaju, Breanna Mann, Rajaneekar Dasari, Emma Livne et autres

Abstract Access to living patient tumor tissue remains a major barrier to accelerating preclinical drug development and informing clinical care. Over the last decade, we have developed a “New Approach Methodology” platform called Screening Live Cancer Explants (SLiCE), which reproducibly engrafts living, …

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Abstract 661: Rapid CAR T testing in an ex vivo platform that maintains passage-zero patient tumor tissues and their native immunosuppressive microenvironment.

Xiaopei Zhang, Breanna Mann, Adebimpe Adefolaju, Alain Valdivia et autres

Abstract Adoptive T cell therapies, including chimeric antigen receptor (CAR) T cells, have shown limited success in solid tumors, largely due to widespread preclinical models that fail to capture patient tumor heterogeneity and the immunosuppressive tumor microenvironment (TME). Conventional systems, including cell …

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Abstract 653: Rapid drug sensitivity testing of passage-zero patient brain tumor tissues to inform preclinical and clinical decisions: results from a clinical feasibility study.

Breanna Mann, Nichole Artz, Adebimpe Adefolaju, Alain Valdivia et autres

Abstract Functional precision medicine (FPM) offers a promising path forward in neuro-oncology, where genomic profiling alone often fails to predict therapeutic response. To bridge this gap, we developed the Screening Live Cancer Explants (SLiCE) platform, a rapid ex vivo drug screening assay …

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High-Resolution 3D Bioprinted Hydrogel Scaffolds Enable Sustained Intraperitoneal Cell Delivery

Yu E. Zhang, Lauren E. Carlberg, Alain Valdivia, Morrent Thang et autres

Intraperitoneal (I.P.) delivery of cell-based therapeutics represents a promising strategy for treating regional peritoneal diseases; however, rapid cellular clearance severely limits therapeutic durability. A critical unmet need is the development of implantable biomaterial platforms that can both mechanically integrate within the dynamic …

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Engineering Ultra‐Soft Porous Hydrogel Scaffolds via High‐Resolution 3D Printing to Enhance Cell‐Based Therapy for Glioblastoma

Josué García-Ávila, Yu Zhang, Lauren Kass, Caroline A. Stockwell et autres

ABSTRACT This paper presents advancements in manufacturing ultra‐soft hydrogel scaffolds via a high‐resolution light‐based vat‐photopolymerization technique called Continuous Liquid Interface Production (CLIP). High‐resolution scaffolds were made using a blend of natural and synthetic hydrogels to enhance printability, structural integrity, and cell‐seeding efficiency. …

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Rapid Functional Drug Screening of Passage-Zero Patient Brain Tumor Tissues Ex Vivo: Results from a Clinical Feasibility Study

Andrew Satterlee, Breanna Mann, Nichole Artz, Adebimpe Adefolaju et autres

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TMOD-34. Results from a clinical feasibility study investigating an ex vivo drug screening platform that tests living, passage-zero brain tumor tissue

Breanna Mann, Nichole Artz, Adebimpe Adefolaju, Alain Valdivia et autres

Abstract Neuro-oncology needs a fundamental shift in how cancer treatments are developed and tailored to individual patients. Despite widespread clinical use of genomic/molecular markers to guide treatment, these approaches have widely failed to predict a patient’s response to treatment. To improve personalized …

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Therapeutic approaches for targeting the pediatric brain tumor microenvironment

Caroline A. Stockwell, Morrent Thang, David E. Kram, Andrew Satterlee et autres

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Integrating IL-12 mRNA nanotechnology with SBRT eliminates T cell exhaustion in preclinical models of pancreatic cancer

Angela L. Hughson, Gary Hannon, Noah Salama, Tara G Vrooman et autres

Pronounced T cell exhaustion characterizes immunosuppressive tumors, with the tumor microenvironment (TME) employing multiple mechanisms to elicit this suppression. Traditional immunotherapies, such as immune checkpoint blockade, often fail due to their focus primarily on T cells. To overcome this, we utilized a …

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Local Delivery of SBRT and IL12 by mRNA Technology Overcomes Immunosuppressive Barriers to Eliminate Pancreatic Cancer

Angela L. Hughson, Gary Hannon, Noah Salama, Tara G Vrooman et autres

The immunosuppressive milieu in pancreatic cancer (PC) is a significant hurdle to treatments, resulting in survival statistics that have barely changed in 5 decades. Here we present a combination treatment consisting of stereotactic body radiation therapy (SBRT) and IL-12 mRNA lipid nanoparticles …

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Phenotype stability, expansion potential, and senescence of embryonic tendon cells in vitro

Phong K. Nguyen, Feiyang Deng, Sereen S. Assi, Paolo Paco et autres

Embryonic tendon cells have been studied in vitro to better understand mechanisms of tendon development. Outcomes of in vitro cell culture studies are easily affected by phenotype instability of embryonic tendon cells during expansion in vitro to achieve desired cell numbers, yet …

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