Kostas D. Mathiopoulos

CRISPR/Cas9‐based white pupae mutant lines in Bactrocera spp. for sterile insect technique applications

Chrysanthi Ioannidou, Maria‐Eleni Gregoriou, Marc F. Schetelig, Elena Drosopoulou et autres

The Bactrocera genus includes highly invasive fruit and vegetable pest species such as Bactrocera dorsalis, Bactrocera correcta, and Bactrocera oleae. The sterile insect technique (SIT) is a biological control method used to suppress populations of the invasive Bactrocera fruit flies by releasing …

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Detection of sex chromosomes in Tephritid pests using R‐CQ and KAMY, two computational methods to support generic pest management applications

Dimitris Rallis, Konstantina T. Tsoumani, Flávia Krsticevic, Philippos Aris Papathanos et autres

The detection and characterization of sex chromosome sequences is particularly important for major pest families, like the Tephritidae, whereas alternative pest management approaches, mainly involving male-only release programs, rely on the ability to target and manipulate sex-specific genomic regions, particularly those of …

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Long-read RNA-seq delineates temporal transcriptional dynamics in multiplexed and sexed single medfly embryos

Anthony Bayega, Spyros Oikonomopoulos, Dimitris Rallis, Dionisia Mavritsakis et autres

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Genome assembly of five Tephritid species for the enhancement of the Sterile Insect Technique

Shu‐Huang Chen, Pierre R. Bérubé, Maria‐Eleni Gregoriou, Marc F. Schetelig et autres

Abstract Tephritidae insect pests account for extensive crop damage and yield losses globally. Modern, sustainable pest management approaches are species-specific and, therefore, high-quality genome assemblies are required for their application. Here, we present chromosome-level assemblies for five members of the Tephritidae family: …

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CRISPR/Cas9‐mediated mutagenesis of the white‐eye gene in the tephritid pest Bactrocera zonata

Albert Nazarov, Tamir Partosh, Flávia Krsticevic, Dimitris Rallis et autres

Bactrocera zonata is a highly invasive agricultural pest that causes extensive damage to fruit crops. The Sterile Insect Technique (SIT), a species-specific and environmentally friendly pest control method, significantly benefits from the availability of Genetic Sexing Strains (GSSs) that enable efficient mass …

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CRISPR/Cas9-mediated mutagenesis of the white-eye gene in the tephritid pest Bactrocera zonata

Albert Nazarov, Tamir Partosh, Flávia Krsticevic, Dimitris Rallis et autres

Abstract Bactrocera zonata is a highly invasive agricultural pest that causes extensive damage to fruit crops. The Sterile Insect Technique (SIT), a species-specific and environmentally friendly pest control method, depends on the availability of Genetic Sexing Strains (GSSs) to enable efficient mass …

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Detection of sex chromosomes in Tephritid pests using R-CQ and KAMY, two computational methods to enable species specific control

Dimitris Rallis, Konstantina T. Tsoumani, Flávia Krsticevic, Philippos Aris Papathanos et autres

Abstract The detection and characterization of sex chromosome sequences is particularly important for major pest families, like the Tephritidae, whereas alternative pest management approaches, mainly involving male-only release programs, rely on the ability to target and manipulate sex-specific genomic regions, particularly those …

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Long non-coding RNAs regulate Aedes aegypti vector competence for Zika virus and reproduction

Alexandros Belavilas-Trovas, Spyros Tastsoglou, Shengzhang Dong, Mary Kefi et autres

Long non-coding RNAs (lncRNAs) play critical regulatory roles in various cellular and metabolic processes in mosquitoes and all other organisms studied thus far. In particular, their involvement in essential processes such as reproduction makes them potential targets for the development of novel …

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Development of Toehold Switches as a Novel Ribodiagnostic Method for West Nile Virus

Antonis Giakountis, Zoe Stylianidou, Anxhela Zaka, Styliani Pappa et autres

. Naturally, the virus is maintained in an enzootic cycle involving mosquitoes as vectors and birds that are the principal amplifying virus hosts. In humans, the incubation period for WNV disease ranges from 3 to 14 days, with an estimated 80% of …

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A species-specific lncRNA modulates the reproductive ability of the asian tiger mosquito

Alexandros Belavilas-Trovas, Maria‐Eleni Gregoriou, Spyros Tastsoglou, Olga Soukia et autres

Long non-coding RNA (lncRNA) research has emerged as an independent scientific field in recent years. Despite their association with critical cellular and metabolic processes in plenty of organisms, lncRNAs are still a largely unexplored area in mosquito research. We propose that they …

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A species-specific lncRNA modulates the reproductive ability of the Asian tiger mosquito

Alexandros Belavilas-Trovas, Maria‐Eleni Gregoriou, Spyros Tastsoglou, Olga Soukia et autres

Abstract Long non-coding RNA (lncRNA) research has emerged as an independent scientific field in recent years. Despite their association with critical cellular and metabolic processes in plenty of organisms, lncRNAs are still a largely unexplored area in mosquito research. We propose that …

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Additional file 3 of The molecular biology of the olive fly comes of age

Efthimia Sagri, Martin Reczko, Konstantina T. Tsoumani, Maria‐Eleni Gregoriou et autres

Additional File 1: (XLSX 26 KB)

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