Dafni Chondronasiou

SENOMORPHIC EFFECT OF GENETIC AND CHEMICAL PARTIAL REPROGRAMMING

Victor Nuñez-Quintela, Jérémy Chantrel, Miguel Angel Prados, Pablo Pedrosa et autres

Partial reprogramming has emerged as a promising strategy to ameliorate aging phenotypes, yet its cellular targets and mechanisms remain poorly defined. Cellular senescence is a central hallmark of aging and a plausible mediator of reprogramming-induced rejuvenation. Here we show that genetic and …

us, fr, es, gb, no, de (code pays fourni par la source)

Vitamin B12 is a limiting factor for induced cellular plasticity and tissue repair

Marta Kovatcheva, Elena Melendez, Dafni Chondronasiou, Federico Pietrocola et autres

Abstract Transient reprogramming by the expression of OCT4, SOX2, KLF4 and MYC (OSKM) is a therapeutic strategy for tissue regeneration and rejuvenation, but little is known about its metabolic requirements. Here we show that OSKM reprogramming in mice causes a global depletion …

es, se, fr, gb (code pays fourni par la source)

Data from High and Interrelated Rates of PD-L1+CD14+ Antigen-Presenting Cells and Regulatory T Cells Mark the Microenvironment of Metastatic Lymph Nodes from Patients with Cervical Cancer

A. Marijne Heeren, Bas D. Koster, Sanne Samuels, Debbie M. Ferns et autres

Abstract A better understanding of the microenvironment in relation to lymph node metastasis is essential for the development of effective immunotherapeutic strategies against cervical cancer. In the present study, we investigated the microenvironment of tumor-draining lymph nodes of patients with cervical cancer …

Supplementary Figure 1 from High and Interrelated Rates of PD-L1+CD14+ Antigen-Presenting Cells and Regulatory T Cells Mark the Microenvironment of Metastatic Lymph Nodes from Patients with Cervical Cancer

A. Marijne Heeren, Bas D. Koster, Sanne Samuels, Debbie M. Ferns et autres

Triple immunofluorescence staining of CD14 (green), CD163 (red) and PD-L1 (blue) of representative tumor negative (LN-) and positive (LN+) lymph node specimen reveals the presence of elevated numbers of CD14+CD163+ cells expressing PD-L1 in LN+ and low to non-detectable expression levels of …

Supplementary Figure 1 from High and Interrelated Rates of PD-L1+CD14+ Antigen-Presenting Cells and Regulatory T Cells Mark the Microenvironment of Metastatic Lymph Nodes from Patients with Cervical Cancer

A. Marijne Heeren, Bas D. Koster, Sanne Samuels, Debbie M. Ferns et autres

Triple immunofluorescence staining of CD14 (green), CD163 (red) and PD-L1 (blue) of representative tumor negative (LN-) and positive (LN+) lymph node specimen reveals the presence of elevated numbers of CD14+CD163+ cells expressing PD-L1 in LN+ and low to non-detectable expression levels of …

Data from High and Interrelated Rates of PD-L1+CD14+ Antigen-Presenting Cells and Regulatory T Cells Mark the Microenvironment of Metastatic Lymph Nodes from Patients with Cervical Cancer

A. Marijne Heeren, Bas D. Koster, Sanne Samuels, Debbie M. Ferns et autres

Abstract A better understanding of the microenvironment in relation to lymph node metastasis is essential for the development of effective immunotherapeutic strategies against cervical cancer. In the present study, we investigated the microenvironment of tumor-draining lymph nodes of patients with cervical cancer …

Pseudoalignment tools as an efficient alternative to detect repeated transposable elements in scRNAseq data

Jaime Martínez de Villarreal, Mark Kalisz, Gabriel Piedrafita, Osvaldo Graña‐Castro et autres

MOTIVATION: Transposable elements (TE) have played a major role in configuring the structures of mammalian genomes through evolution. In normal conditions, the expression of these elements is repressed by different epigenetic regulation mechanisms such as DNA methylation, histone modification and regulation by …

es (code pays fourni par la source)

Deciphering the roadmap of in vivo reprogramming toward pluripotency

Dafni Chondronasiou, Jaime Martínez de Villarreal, Elena Melendez, Cian J. Lynch et autres

Differentiated cells can be converted into pluripotent stem cells by expressing the transcription factors OCT4, SOX2, KLF4, and MYC (OSKM) in a process known as reprogramming. Here, using single-cell RNA sequencing of pancreas undergoing reprogramming, we identify markers along the trajectory from …

es (code pays fourni par la source)

Local delivery of low-dose anti–CTLA-4 to the melanoma lymphatic basin leads to systemic T reg reduction and effector T cell activation

Kim M. van Pul, Jessica C.L. Notohardjo, Marieke F. Fransen, Bas D. Koster et autres

Preclinical studies show that locoregional CTLA-4 blockade is equally effective in inducing tumor eradication as systemic delivery, without the added risk of immune-related side effects. This efficacy is related to access of the CTLA-4 blocking antibodies to tumor-draining lymph nodes (TDLNs). Local …

fr, ca, nl, us (code pays fourni par la source)

Deciphering the roadmap of in vivo reprogramming towards pluripotency

Dafni Chondronasiou, Jaime Martínez de Villarreal, Elena Melendez, Cian J. Lynch et autres

Summary Differentiated cells can be converted to pluripotent stem cells (iPSCs) upon ectopic expression of transcription factors OCT4, SOX2, KLF4 and MYC (OSKM) in a process known as reprogramming. Great efforts have been made to dissect intermediate states of in vitro reprogramming …

es (code pays fourni par la source)

Multi‐omic rejuvenation of naturally aged tissues by a single cycle of transient reprogramming

Dafni Chondronasiou, Diljeet Gill, Lluc Mosteiro, Rocío G. Urdinguio et autres

The expression of the pluripotency factors OCT4, SOX2, KLF4, and MYC (OSKM) can convert somatic differentiated cells into pluripotent stem cells in a process known as reprogramming. Notably, partial and reversible reprogramming does not change cell identity but can reverse markers of …

es, gb, us, fr, se, cn (code pays fourni par la source)

Multi-omic rejuvenation of naturally aged tissues by a single cycle of transient reprogramming

Dafni Chondronasiou, Diljeet Gill, Lluc Mosteiro, Rocío G. Urdinguio et autres

ABSTRACT The expression of the pluripotency factors OCT4, SOX2, KLF4 and MYC (OSKM) can convert somatic differentiated cells into pluripotent stem cells in a process known as reprogramming. Notably, cycles of brief OSKM expression do not change cell identity but can reverse …

es, gb, fr, se, cn (code pays fourni par la source)