Martha Evans-Holm

Abstract 332: Improved high-throughput genomic analysis of urine samples using pixelated-ultrasound to enable personalized genomic profiling and therapeutic developments in cancer

Martha Evans-Holm, Nicolai P. Lumbres, Rwik Sen, Patricia Montilla‐Perez et autres

Abstract Here we address some of the existing challenges in liquid biopsy workflows which can potentially benefit urine analysis from bladder cancer patients because approximately 1/4th of all human cancers are urological tumors. Globally, bladder cancer is the 10th most prevalent, and …

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A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila

Sonal Nagarkar-Jaiswal, Pei-Tseng Lee, Megan E. Campbell, Kuchuan Chen et autres

Here, we document a collection of ∼7434 MiMIC (Minos Mediated Integration Cassette) insertions of which 2854 are inserted in coding introns. They allowed us to create a library of 400 GFP-tagged genes. We show that 72% of internally tagged proteins are functional, …

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Author response: A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila

Sonal Nagarkar-Jaiswal, Pei-Tseng Lee, Megan E. Campbell, Kuchuan Chen et autres

A large collection of functional EGFP tagged proteins derived from MiMIC insertions allows examination of protein expression in unfixed tissues and efficient tissue specific reversible knock down of proteins.

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MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes

Koen J. T. Venken, Karen L. Schulze, Nele A.T Haelterman, Hongling Pan et autres

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The Drosophila Gene Disruption Project: Progress Using Transposons With Distinctive Site Specificities

Hugo Jozef Bellen, Robert W. Levis, Yuchun He, Joseph W. Carlson et autres

The Drosophila Gene Disruption Project (GDP) has created a public collection of mutant strains containing single transposon insertions associated with different genes. These strains often disrupt gene function directly, allow production of new alleles, and have many other applications for analyzing gene …

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Sequence finishing and mapping of Drosophila melanogaster heterochromatin.

Roger A. Hoskins, Joseph W. Carlson, Cameron Kennedy, Daniel Estrada Acevedo et autres

Genome sequences for most metazoans and plants are incomplete because of the presence of repeated DNA in the heterochromatin. The heterochromatic regions of Drosophila melanogaster contain 20 million bases (Mb) of sequence amenable to mapping, sequence assembly, and finishing. We describe the …

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Sequence Finishing and Mapping of Drosophila melanogaster Heterochromatin

Roger A. Hoskins, Joseph W. Carlson, Cameron Kennedy, Daniel Estrada Acevedo et autres

Genome sequences for most metazoans and plants are incomplete because of the presence of repeated DNA in the heterochromatin. The heterochromatic regions of Drosophila melanogaster contain 20 million bases (Mb) of sequence amenable to mapping, sequence assembly, and finishing. We describe the …

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Identification of putative noncoding polyadenylated transcripts in Drosophila melanogaster

Jonathan L. Tupy, Adina M. Bailey, Gina M. Dailey, Martha Evans-Holm et autres

Analysis of EST and cDNA collections from a number of metazoan species has identified genes encoding long polyadenylated transcripts that do not contain ORFs of lengths typical for protein-encoding mRNAs. Noncoding functions of such polyadenylated transcripts have been elucidated in only a …

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A computational and experimental approach to validating annotations and gene predictions in the Drosophila melanogaster genome

Mark D. Yandell, Adina M. Bailey, Sima Misra, Shengqiang Shu et autres

Five years after the completion of the sequence of the Drosophila melanogaster genome, the number of protein-coding genes it contains remains a matter of debate; the number of computational gene predictions greatly exceeds the number of validated gene annotations. We have assembled …

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The BDGP Gene Disruption Project

Hugo Jozef Bellen, Robert W. Levis, Guochun Liao, Yuchun He et autres

The Berkeley Drosophila Genome Project (BDGP) strives to disrupt each Drosophila gene by the insertion of a single transposable element. As part of this effort, transposons in >30,000 fly strains were localized and analyzed relative to predicted Drosophila gene structures. Approximately 6300 …

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A Drosophila Complementary DNA Resource

Gerald M. Rubin, Ling Hong, Peter B Brokstein, Martha Evans-Holm et autres

Collections of nonredundant, full-length complementary DNA (cDNA) clones for each of the model organisms and humans will be important resources for studies of gene structure and function. We describe a general strategy for producing such collections and its implementation, which so far …

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A POORLY DIFFERENTIATED LYMPHOMA OF DONOR ORIGIN IN A RENAL ALLOGRAFT RECIPIENT

BRIAN HJELLE, Martha Evans-Holm, T. S. Benedict Yen, Marvin R. Garovoy et autres

Malignant lymphoma is a frequent complication of organ transplantation. It has been suggested that such tumors arise as a result of uncontrolled proliferation of Epstein-Barr virus-infected B lymphocytes in an immunosuppressed host. Although a few cases of posttransplant lymphomas in bone marrow …

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