Matthew P. Spindler

Combining genotoxic gut bacterial strains increases tumor burden and accelerates onset in a germ-free mouse model of colon carcinogenesis

Tamar Plitt, Alix Piessevaux, Urvija Rajpal, Jeremy Fischer et autres

) with 19 cultured human fecal microbiotas from healthy individuals and patients with inflammatory bowel disease or colorectal cancer. Colonic tumor counts vary by donor microbiota but not by donor health status. In vitro screens of host cell proliferation, genotoxicity, and inflammation …

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T Cell Clonal Groups are Broadly Dispersed in Colon, Phenotypically Diverse, and Altered in Ulcerative Colitis

Jeremy Fischer, Matthew P. Spindler, G J Britton, Jared Weiler et autres

Abstract Our understanding of human mucosal T cell clonotype distribution in health and disease has centered on immunodominant antigens. We performed single cell T cell receptor (TCR) and RNA sequencing as an untargeted approach to define distributions of T cell clonal groups …

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Cooperation Between Genotoxic Bacteria Accelerates Tumorigenesis in a Mouse Model of Colon Carcinogenesis

Tamar Plitt, Alix Piessevaux, Urvija Rajpal, Jeremy Fisher et autres

ABSTRACT To identify causal links between gut microbes and tumorigenesis, we colonized groups of germ-free colon tumor-susceptible mice ( Apc Min/+ ; Il10 −/− ) with 15 cultured human fecal microbiotas from healthy individuals, as well as patients with inflammatory bowel disease …

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The protozoan commensal Tritrichomonas musculis is a natural adjuvant for mucosal IgA

Eric Yixiao Cao, Kyle Burrows, Pailin Chiaranunt, Ana Popovic et autres

Immunoglobulin (Ig) A supports mucosal immune homeostasis and host-microbiota interactions. While commensal bacteria are known for their ability to promote IgA, the role of non-bacterial commensal microbes in the induction of IgA remains elusive. Here, we demonstrate that permanent colonization with the …

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The Case for Continuous Professional Development in Accessible Course Design

Matthew P. Spindler, Kristina Wendricks

This chapter proposes social validity theory as a framework for designing and evaluating professional development on accessibility. It introduces a four-part professional development program for practitioners on the socially important topic of accessibility. Social validity frames and evaluates the social acceptability of …

Species-specific CD4 + T cells enable prediction of mucosal immune phenotypes from microbiota composition

Matthew P. Spindler, Ilaria Mogno, Prerna Suri, Graham J. Britton et autres

How bacterial strains within a complex human microbiota collectively shape intestinal T cell homeostasis is not well understood. Methods that quickly identify effector strains or species that drive specific mucosal T cell phenotypes are needed to define general principles for how the …

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The protozoan commensal Tritrichomonas musculis is a natural adjuvant for mucosal IgA

Eric Yixiao Cao, Kyle Burrows, Pailin Chiaranunt, Ana Popovic et autres

ABSTRACT Immunoglobulin(Ig) A antibodies are the most abundant antibodies supporting mucosal immune homeostasis and host-microbiota interactions. Driven by gut commensal microbes, IgA-secreting plasma cells (PC) differentiate through T cell-dependent (Td) or T cell independent (Ti) mechanisms. While commensal bacteria within the microbiota …

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Human gut microbiota stimulate defined innate immune responses that vary from phylum to strain

Matthew P. Spindler, Sophia Siu, Ilaria Mogno, Zhihua Li et autres

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Species-specific CD4 + T cells Enable Prediction of Mucosal Immune Phenotypes from Microbiota Composition

Matthew P. Spindler, Ilaria Mogno, Prerna Suri, Graham J. Britton et autres

ABSTRACT How bacterial strains within a complex human microbiota collectively shape intestinal T cell homeostasis is not well-understood. Methods that quickly identify effector strains or species that drive specific mucosal T cell phenotypes are needed to define general principles for how the …

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Immunoglobulin A antibody composition is sculpted to bind the self gut microbiome

Chao Yang, Alice Chen-Liaw, Matthew P. Spindler, Domenico Tortorella et autres

Despite being the most abundantly secreted immunoglobulin isotype, the pattern of reactivity of immunoglobulin A (IgA) antibodies toward each individual's own gut commensal bacteria still remains elusive. By colonizing germ-free mice with defined commensal bacteria, we found that the binding specificity of …

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Gut clues to weight gain after quitting smoking

Matthew P. Spindler, Jeremiah J. Faith, Junshi Wang, Paul J. Kenny

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Defining Innate Immune Responses to the Human Gut Microbiota from Phylum to Strain

Matthew P. Spindler, Sophia Siu, Ilaria Mogno, Zhihua Li et autres

SUMMARY The functional potential of the gut microbiota remains largely uncharacterized. Efforts to understand how the immune system responds to commensal organisms have been hindered by the large number of strains that comprise the human gut microbiota. We develop a screening platform …

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