Liben Chen

A streamlined multiplexed digital mRNA quantification platform with universal primers and limited fluorescence channels

Joon Soo Park, Jiumei Hu, Liben Chen, Tza-Huei Wang

Multiplexed RNA quantification is a crucial tool in various biological fields, but current PCR-based methods often face challenges in primer design, amplification bias, and limited fluorescence labels for multiplexing. Here, we introduce Fluorescence-coding extension PCR (FlexPCR), a novel digital PCR-based assay that …

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Simple dual filter workflow for facilitating blood culture-free and sensitive detection of pathogenic bacteria from blood

Dong Jin M Park, Tian-qi Wu, Liben Chen, Pei‐Wei Lee et autres

Bloodstream infections (BSI) caused by pathogenic bacteria are a global health threat, highlighting the need for improved diagnostic methods. Current methods, such as mass spectrometry, depend on time-consuming blood cultures, which hinder timely diagnosis. Nucleic acid amplification tests (NAATs) offer potential for …

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FlexPCR: A streamlined multiplexed digital mRNA quantification platform with universal primers and limited fluorescence channels

Joon Soo Park, Jiumei Hu, Liben Chen, Tza‐Huei Wang

Accurate quantification of multiple messenger RNA (mRNA) targets is essential for biomedical research and disease diagnosis. Current PCR-based methods for mRNA analysis are limited by the number of fluorescent labels and the complexities associated with multiple target-specific primers, leading to amplification bias …

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From crisis to innovation in point-of-care testing: Lessons from the COVID-19 pandemic and future directions

Xiaoxiao Lu, Jing Du, Minli You, Liben Chen et autres

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Exploiting β‐Lactams‐Induced Lysis and DNA Fragmentation for Rapid Molecular Antimicrobial Susceptibility Testing of Neisseria Gonorrhoeae via Dual‐Digital PCR

Jiumei Hu, Liben Chen, Pengfei Zhang, Fan‐En Chen et autres

The evolution of antimicrobial resistance (AMR) presents substantial challenges to global medical health systems. Neisseria gonorrhoeae (N. gonorrhoeae), in particular, has developed resistance to all currently available antimicrobials. Addressing this issue necessitates not only discovering new antimicrobials but also deepening the understanding …

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Automatic Detection of Alzheimer's Disease with Multi-Modal Fusion of Clinical MRI Scans

Long Chen, Liben Chen, Binfeng Xu, Wenxin Zhang et autres

The aging population of the U.S. drives the prevalence of Alzheimer's disease. Brookmeyer et al. forecasts approximately 15 million Americans will have either clinical AD or mild cognitive impairment by 2060. In response to this urgent call, methods for early detection of …

Facile Filtration-Based Workflow for Facilitating Sensitive Detection of Pathogenic Bacteria from Blood

Dong Jin M Park, Liben Chen, Tian-qi Wu, Pei‐Wei Lee et autres

Abstract Bloodstream infections (BSIs) are a global health concern, contributing to high mortality rates and increased healthcare costs. Current diagnostic methods, such as blood culture, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), immunoassays, and nucleic acid amplification tests (NAATs), have lengthy …

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Harnessing Variabilities in Digital Melt Curves for Accurate Identification of Bacteria

Pei‐Wei Lee, Liben Chen, Kuangwen Hsieh, Amelia Traylor et autres

Digital PCR combined with high resolution melt (HRM) is an emerging method for identifying pathogenic bacteria with single cell resolution via species-specific digital melt curves. Currently, the development of such digital PCR-HRM assays entails first identifying PCR primers to target hypervariable gene …

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On the Cognition of Visual Question Answering Models and Human Intelligence: A Comparative Study

Liben Chen, Long Chen, Tian Ellison-Chen, Zhuoyuan Xu

Visual Question Answering (VQA) is a challenging task that requires cross-modal understanding and reasoning of visual image and natural language question. To inspect the association of VQA models to human cognition, we designed a survey to record human thinking process and analyzed …

Sensitive and Quantitative Point-of-Care HIV Viral Load Quantification from Blood Using a Power-Free Plasma Separation and Portable Magnetofluidic Polymerase Chain Reaction Instrument

Hoan T. Ngo, Mei Jin, Alexander Y. Trick, Fan‐En Chen et autres

Point-of-care (POC) HIV viral load (VL) tests are needed to enhance access to HIV VL testing in low- and middle-income countries (LMICs) and to enable HIV VL self-testing at home, which in turn have the potential to enhance the global management of …

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Digital Ligation-Enabled Fluorescence-Coding PCR (dLiNC PCR) for High-Dimensional Multiplexed Nucleic Acid Detection

Joon Soo Park, Liben Chen, Tza‐Huei Wang

Polymerase chain reaction (PCR) is the most commonly used method for nucleic acid amplification due to its ability to detect trace levels of target molecules. However, the development of multiplexed PCR assays has been limited by the complexity of the assay derived …

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Highly Sensitive Serum Protein Analysis Using Magnetic Bead-Based Proximity Extension Assay

Pengfei Zhang, Jiumei Hu, Joon Soo Park, Kuangwen Hsieh et autres

Many protein biomarkers are present in biofluids at a very low level but may play critical roles in important biological processes. The fact that these low-abundance proteins remain largely unexplored underscores the importance of developing new tools for highly sensitive protein detection. …

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