Inna Goliand

Spatial analysis reveals the evolving organization of IDH-mutant glioma

Rouven Hoefflin, Alissa R. Greenwald, Noam Darnell, Christopher Mount et autres

Adult diffuse gliomas are composed of malignant cell states interwoven with the non-malignant brain microenvironment. Here, we combine spatial transcriptomics and spatial proteomics of isocitrate dehydrogenase (IDH)-mutant gliomas to define organizational principles across histological grades. In low-grade tumors, spatial organization is shaped …

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Engineering Receptor-Mediated Cell-Surface Mobility with Phage-Inspired DNA Tetrapods

Gal Raviv, Ilan Edelstein, Tali Dadosh, Inna Goliand et autres

Viruses have long inspired the development of multiligand display systems that engage cell-surface receptors through strong multivalent interactions. However, considerably less attention has been devoted to recapitulating the early stages of infection, during which weak, reversible interactions promote receptor-mediated mobility across cell …

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PDE5A Inhibition Restricts Cancer Metastasis by Disrupting NPC1-Mediated Cholesterol Trafficking through a Noncanonical cGMP-Dependent Pathway

Yarden Ariav, Samah Hayek, Thomas Cantore, Neel Sanghvi et autres

Abstract Noncanonical metabolic functions of signaling molecules contribute to cancer plasticity and metastatic progression. In this study, we demonstrated that phosphodiesterase type 5A (PDE5A) inhibitors, including sildenafil (Sild; Viagra), induced lysosomal cholesterol accumulation across multiple mouse and human cancer models, reducing cholesterol …

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Supplementary Figure 6 from Temporal Genomic Analysis of Homogeneous Tumor Models Reveals Key Regulators of Immune Evasion in Melanoma

Sapir Cohen Shvefel, Joy A. Pai, Yingying Cao, Lipika R. Pal et autres

Differential expression analysis of rejected vs. non-rejected tumor cells.

Supplementary Figure 5 from Temporal Genomic Analysis of Homogeneous Tumor Models Reveals Key Regulators of Immune Evasion in Melanoma

Sapir Cohen Shvefel, Joy A. Pai, Yingying Cao, Lipika R. Pal et autres

CyTOF analysis confirms high M2-like macrophage infiltration and T-cell exhaustion in non-rejected single-cell clones.

Supplementary Figure 13 from Temporal Genomic Analysis of Homogeneous Tumor Models Reveals Key Regulators of Immune Evasion in Melanoma

Sapir Cohen Shvefel, Joy A. Pai, Yingying Cao, Lipika R. Pal et autres

scRNA-seq characterization of Mif KO tumor clones.

Supplementary Table 9 from Temporal Genomic Analysis of Homogeneous Tumor Models Reveals Key Regulators of Immune Evasion in Melanoma

Sapir Cohen Shvefel, Joy A. Pai, Yingying Cao, Lipika R. Pal et autres

List of antibodies used for CODEX multiplexed tissue imaging

Supplementary Figure 15 from Temporal Genomic Analysis of Homogeneous Tumor Models Reveals Key Regulators of Immune Evasion in Melanoma

Sapir Cohen Shvefel, Joy A. Pai, Yingying Cao, Lipika R. Pal et autres

CyTOF analysis comparing tumors derived from Mif WT and Mif KO validating the scRNAseq findings.

Supplementary Figure 3 from Temporal Genomic Analysis of Homogeneous Tumor Models Reveals Key Regulators of Immune Evasion in Melanoma

Sapir Cohen Shvefel, Joy A. Pai, Yingying Cao, Lipika R. Pal et autres

Cell types from scRNA-seq of tumors from mice inoculated with rejected or non-rejected SCCs.

Supplementary Table 6 from Temporal Genomic Analysis of Homogeneous Tumor Models Reveals Key Regulators of Immune Evasion in Melanoma

Sapir Cohen Shvefel, Joy A. Pai, Yingying Cao, Lipika R. Pal et autres

CRISPR pooled guide library constructs

Supplementary Table 3 from Temporal Genomic Analysis of Homogeneous Tumor Models Reveals Key Regulators of Immune Evasion in Melanoma

Sapir Cohen Shvefel, Joy A. Pai, Yingying Cao, Lipika R. Pal et autres

Pathway enrichment in samples at day 0 post-inoculation.

Supplementary Figure 4 from Temporal Genomic Analysis of Homogeneous Tumor Models Reveals Key Regulators of Immune Evasion in Melanoma

Sapir Cohen Shvefel, Joy A. Pai, Yingying Cao, Lipika R. Pal et autres

Opal and CODEX multiplexed tissue imaging data shows infiltration of proliferative CD8+ T-cells to the rejected SCCs.