George T. Duncan

Applications of digital polymerase chain reaction in environment and forensic chemistry

Sylvia Etim, Dipanwita Raha, George T. Duncan, Bruce Royston McCord

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List of contributors

Joe Adserias‐Garriga, Bhavna Ahlawat, Abdullah Aldasem, Mohammad H. Al-Hasan et autres

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DNA Methylation Markers and the Identification of Buccal Samples for Forensic Tissue Determination

Kuppareddi Balamurugan, E.L.J. Staples, Hussain Alghanim, George T. Duncan et autres

Background: The identification of body fluids collected from crime scenes is crucial for determining the type and nature of assaults and for advancing the resolution of crimes. Objectives: The primary aim of this study was to investigate tissue-specific DNA methylation markers that …

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Forensic insights from shotgun metagenomics: Tracing microbial exchange during sexual intercourse

Mirna Ghemrawi, Andrea Ramírez Torres, Michael D. Netherland, Ying Wang et autres

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Forensic assessment of kinship, genomic ancestry, and natural history of an iconic tiger of Harlem, New York City

Zhiyuan Wu, Zheng-Yan Sui, Lanhui Peng, Mirna Ghemrawi et autres

In the fall of 2003, a 2-yr-old tiger named Ming, weighing around 300 pounds, was discovered living in an apartment in Harlem, New York. Ming's rescue by NYPD was witnessed, recalled, and venerated by scores of neighbors. The tiger's history and ancestry …

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A study of the potential application of digital PCR in the detection of fecal contamination of strawberries using Bacteroides markers

Nicole Fernandez‐Tejero, Alyssa N. Sanchez, Mirna Ghemrawi, Catherine Bilodeau et autres

Food-borne illnesses can result from contamination of agricultural products. In this study, we examined nanoplate digital PCR (dPCR) to test for fecal contamination of agricultural products. In nanoplate technique, the PCR mastermix is divided into 8.526,000 partitions, providing direct detection of individual …

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A Method for the Detection of Fecal Contamination of Strawberries Using Bacteroides Markers and Nanoplate Digital Pcr

Nicole Fernandez‐Tejero, Alyssa N. Sanchez, Mirna Ghemrawi, Catherine Bilodeau et autres

Pyrosequencing: Current forensic methodology and future applications—a review

Mirna Ghemrawi, Nicole Fernandez‐Tejero, George T. Duncan, Bruce Royston McCord

The recent development of small, single-amplicon-based benchtop systems for pyrosequencing has opened up a host of novel procedures for applications in forensic science. Pyrosequencing is a sequencing by synthesis technique, based on chemiluminescent inorganic pyrophosphate detection. This review explains the pyrosequencing workflow …

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Developmental validation of SpeID: A pyrosequencing-based assay for species identification

Mirna Ghemrawi, Frank Fischinger, George T. Duncan, Mary Jones Dukes et autres

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ddPCR Reveals SARS-CoV-2 Variants in Florida Wastewater

Eben Gering, Jacob Colbert, Sarah E. Schmedes, George T. Duncan et autres

Abstract Wastewater was screened for the presence of functionally significant mutations in SARS-CoV-2 associated with emerging variants of concern (VOC) by ddPCR, and results accorded with sequencing of clinical samples from the same region. We propose that PCR-based screening of wastewater can …

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A data‐driven, high‐throughput methodology to determine tissue‐specific differentially methylated regions able to discriminate body fluids

Joana Antunes, Quentin T. Gauthier, Vanessa Aguiar‐Pulido, George T. Duncan et autres

Tissue-specific differentially methylated regions (tDMRs) are regions of the genome with methylation patterns that modulate gene expression in those tissue types. The detection of tDMRs in forensic evidence can permit the identification of body fluids at trace levels. In this report, we …

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BIO-INSPIRED MAGNETIC BEADS FOR ISOLATION OF SPERM FROM HETEROGENOUS SAMPLES IN FORENSIC APPLICATIONS

Fatih İnci, Merve Gökşin Karaaslan, Rakhi Gupta, Anirudh Avadhani et autres

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