Yiyang Zhou

Antagonism of stress granules is key to SARS-CoV-2 infection and pathogenesis

R. Elias Alvarado, Jennifer Chen, Kumari G. Lokugamage, Yiyang Zhou et autres

ABSTRACT Viruses must subvert host responses to facilitate successful infection. While most antiviral responses are associated with interferons, stress granules (SGs) are another barrier to viral infection by inducing translation arrest. To combat SG activity, viruses have evolved mechanisms to disrupt formation …

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Development of low‐prolamin rice germplasm via CRISPR/Cas9 editing to improve eating and cooking quality

Zhihui Chen, Yu Chen, Gexing Wan, Hui Dong et autres

Simultaneous editing of two major prolamin-encoding genes in rice using a single-guide RNA suppressed prolamin accumulation and reconfigured seed storage protein composition. This targeted modification markedly improved rice eating and cooking quality while maintaining the levels of total starch, total protein, and …

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Loss-of-function mutation in Omicron variants reduces spike protein expression and attenuates SARS-CoV-2 infection

Michelle N. Vu, R. Elias Alvarado, Dorothea R. Morris, Kumari G. Lokugamage et autres

SARS-CoV-2 Omicron variants emerged in 2022 with >30 novel mutations in the spike alone. While most studies focus on receptor binding domain changes, mutations in the C-terminus of S1 (CTS1), adjacent to the furin cleavage site, have largely been ignored. Here, we …

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Antagonism of stress granules key for SARS-CoV-2 infection and pathogenesis

R. Elias Alvarado, Jennifer Chen, Kumari G. Lokugamage, Yiyang Zhou et autres

ABSTRACT Viruses must subvert host responses to facilitate successful infection. While most antiviral responses are associated with interferons, stress granules (SG) are another barrier to viral infection by inducing translation arrest. To combat SG activity, viruses have evolved mechanisms to disrupt formation …

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Generation and Application of a Monoclonal Antibody mAb-57P Specifically Recognizing the Precursor form of Glutelins in Rice Endosperm

Wan Gexing, Wenkun Yang, Yiyang Zhou, Pan Tian et autres

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A ligase-mediated encoding system for rapid, multiplex single-nucleotide variant detection

Yuwan Lin, X Y Tang, Tianshu Chen, Xuexue Liu et autres

Accurate detection of single-nucleotide variants (SNV) is critical for genetic disease diagnosis and cancer profiling, yet existing methods often lack the combination of specificity, multiplexing capacity, and rapid workflow required for clinical application. Here, we report a ligase-mediated encoding (LmCo) system that …

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Ordered release of genomic RNA during icosahedral virus disassembly

Yiyang Zhou, Andrew Routh

ABSTRACT Many icosahedral viruses release their genomes through a series of ordered conformational changes involving distinct disassembly intermediates. While previous studies have focused on rearrangements of the capsid protein shell, it remains unclear whether viral genomes undergo defined structural transitions or are …

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SARS-CoV-2 EndoU-ribonuclease regulates RNA recombination and impacts viral fitness

Yiyang Zhou, Yani P. Ahearn, Kumari G. Lokugamage, Angelica L. Morgan et autres

Coronaviruses (CoVs) maintain large RNA genomes that frequently undergo mutations and recombination, contributing to their evolution and emergence. In this study, we find that SARS-CoV-2 has greater RNA recombination frequency than other human CoVs. In addition, CoV RNA recombination primarily occurs at …

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Inhalable Herbal Nano-Liposomes Co-Delivering Astragaloside IV and Polyphyllin VII Reprogram Immunosuppressive Microenvironment To Overcome Anti-PD-1 Resistance in NSCLC

Y. Liu, Tao An, Zujun Que, Bin Luo et autres

Non-small cell lung cancer (NSCLC) frequently develops acquired resistance to immune checkpoint inhibitors (ICIs), necessitating innovative strategies to remodel the immunosuppressive tumor microenvironment (TME). This study engineered an inhalable pH-responsive nano-liposome co-delivering Astragaloside IV (AS-IV) and Polyphyllin VII (Pol VII) (AS-IV/Pol VII-Lipo) …

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Convergent evolution in nucleocapsid facilitated SARS-CoV-2 adaptation for human infection

Kumari G. Lokugamage, Yiyang Zhou, R. Elias Alvarado, Jessica A. Plante et autres

During the early stages of the COVID-19 pandemic, several SARS-CoV-2 variants of concern (VOCs) independently acquired mutations in the highly variable 203-205 amino acid region of the nucleocapsid (N) protein including R203K + G204R (found in the Alpha, Gamma, and Omicron variants), …

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The furin cleavage site is required for pathogenesis, but not transmission, of SARS-CoV-2

Angelica L. Morgan, Michelle N. Vu, Yiyang Zhou, Kumari G. Lokugamage et autres

ABSTRACT The SARS-CoV-2 spike, key to viral entry, has two features that differentiate it from other sarbecoviruses: the presence of a furin cleavage site (FCS; PRRAR sequence) and an extended S1/S2 loop characterized by an upstream QTQTN amino acid motif. Our prior …

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The furin cleavage site is required for pathogenesis, but not transmission of SARS-CoV-2

Angelica L. Morgan, Michelle N. Vu, Yiyang Zhou, Kumari G. Lokugamage et autres

Abstract The SARS-CoV-2 spike, key to viral entry, has two features that differentiate it from other sarbecoviruses: the presence of a furin cleavage site (FCS; PRRAR sequence) and an extended S1/S2 loop characterized by an upstream QTQTN amino acid motif. Our prior …

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