Shouwen Chen

System reconstruction of Bacillus licheniformis for efficient expression of alkaline protease

Qing Zhang, Mengyuan Zhang, Zhihao Zhu, Haijing Ma et autres

Industrial enzymes are widely used in diverse applications, but low productivity limits their further widespread utilization. This research aimed to develop high-performance alkaline protease (AprE) expression strains of Bacillus licheniformis through element optimization and modular engineering. Firstly, the aprE gene expression cassette …

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Development of a bifunctional dynamic control system for effective product synthesis in Bacillus licheniformis DW2

Yi Rao, Yi-Xiang Wang, J F Liu, Rui Zhao et autres

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Protein engineering combined with combinatorial optimization of critical synthetase cluster for efficient production of 1-Deoxynojirimycin in B. amyloliquefaciens

Xujie Li, Xin Feng, Aoying Dong, Shihao Yan et autres

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Multidimensional Metabolic Engineering for Green and Sustainable Bioproduction of Serotonin from Glucose in Bacillus licheniformis

Xinyuan Yang, Yue Wang, Ji Li, Wenyuan Liu et autres

Serotonin (5-hydroxytryptamine, 5-HT) plays a critical role in maintaining intestinal health, regulating mood and sleep, and enhancing crop stress tolerance. Here, a Bacillus licheniformis cell factory was developed for the sustainable and efficient synthesis of 5-HT. First, a tryptophan-producing chassis strain, TRP18, …

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Microbubble enhanced CO2-to-ethanol conversion for artificial CC condensation pathways

Wanrong Dong, Xiuling Ji, Boxia Guo, Haijun Yu et autres

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GSDME unlocks astrocyte-driven neurotoxicity in Alzheimer’s Disease

Xueman Xie, Chenhui Ji, Jingmei Xu, Xiaoyang Lu et autres

SUMMARY Astrocytic calcium dysregulation and reactivity precede Aβ deposition in amyloid-β deposition in Alzheimer’s disease (AD) but the neurotoxic mechanisms remain unclear. We show that GSDME acts as a switch, linking MAM-mediated calcium release to astrocyte-driven neurotoxicity. Specifically, Aβ-activated microglial signals activate …

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Unraveling the chrono-regulatory function of PhoP in nitrogen metabolism: A multi-scale engineering framework aimed at optimizing the synthesis of poly-γ-glutamic acid in Bacillus licheniformis

Qing Zhang, Shisi He, Fangzhou Cheng, Yaping Jin et autres

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Elucidating metabolic mechanisms underlying the influence of specific growth rate on alkaline protease synthesis in Bacillus licheniformis through combined omics and computational modeling analysis

Ying Zhang, Zhihao Liu, Jingmin Hu, Hongfei Xu et autres

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An application of YOLOv8 integrated with attention mechanisms for detection of grape leaf black rot spots

Jiajun Zhu, Jinlin Qiu, Shouwen Chen, Shuxi Chen et autres

Aiming at the problems of low recognition rate of small target spots in grape leaf images and low detection accuracy due to low resolution of input images. In this paper, an improved recognition network based on YOLO v8 is constructed. In the …

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Optimizing genome editing efficiency in Streptomyces fradiae via a CRISPR/Cas9n-mediated editing system

Yanmin Wu, Hui Jin, Qiang Yu, Zihan Wei et autres

ABSTRACT Streptomyces fradiae is an important bioresource to produce various antibacterial natural products, however, the time-consuming and labor-intensive genome editing toolkits hindered the construction and application of engineered strains, and this study aimed to establish an efficient CRISPR/Cas9n genome editing system in …

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Efficient sustainable production of protocatechuic acid from glucose by engineered Bacillus licheniformis

Yi Rao, Jiaqi Wang, Rui Zhao, Yangyang Zhan et autres

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Metabolic engineering of Bacillus licheniformis DW2 for ectoine production

Xujie Li, Dong An, Yang Jun-ru, Jiang Zhu et autres

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