Zhifeng Fu

Bacteriophage and Urease-Conjugated Janus Micromotors for Dynamic Enrichment and Sensitive Detection of Bacteria

Zhirou Zhai, Honglin Yang, Yumeng Li, Ruining Bai et autres

Abstract It is highly demanded to establish effective and sensitive method for detecting pathogenic bacteria because of their high morbidity. The conventional molecular recognition-based methods rely on heterogeneous mode, for which the passive diffusion of bacterial cells and signal tracer results in …

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Recombinant Phage Tail Fiber Protein Gp47 Enables Broad-Spectrum Fluorescent Detection of Pseudomonas aeruginosa Strains

Honglin Yang, Qinchen Liao, Kuixun CHEN, Xiaodong Chen et autres

Pseudomonas aeruginosa ( P. aeruginosa ) has long been one of the leading opportunistic pathogens responsible for serious healthcare-associated infective diseases. Development of rapid and accurate detection techniques for this pathogen is highly desired to achieve efficient prevention and control. This study …

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Highly amphiphilicity-tailorable pineapple-like janus nanozymes with superior kinematic locomotion facilitates biphasic chemiluminescence analysis

Xin Jiang, Wan Wei, Yueming Zhao, Lingli Zhang et autres

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Prp8 Intein-Targeted Fluorescent Probe for Specific Detection of Cryptococcus neoformans and Inhibitor Screening

Ting Guo, Fang Wen, Yulin Feng, Wenjie Huang et autres

Cryptococcus neoformans ( C. neoformans ) infections pose a critical threat to global health, with high mortality rates observed among immunocompromised individuals. Rapid, cost-effective diagnostics are essential for preventive treatment and reducing mortality in resource-scarce settings, yet the development of selective fluorescent …

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Pt single-atom catalyst-Enhanced aerogenous reaction for pressure signal-based immunochromatographic test strip

Jinxia Xue, Shiruoyu Zhou, Chunjie Zhou, Essam Abdo Mohammed Saad Obaid et autres

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pH-responsive smart hydrogel dressing for controlled antimicrobial therapy and accelerated wound healing

Essam Abdo Mohammed Saad Obaid, Ruining Bai, Zhifeng Fu

) strains, with effective biofilm suppression. Biocompatibility assays using human colorectal adenocarcinoma cells (Caco-2) and mouse fibroblast cells (L929) validated cytocompatibility. This facile and scalable platform offers a promising therapeutic strategy for infected wounds.

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Dopamine-Modulated Highly Oxygen-Vacancy Ce Metal–Organic Frameworks Boost Phosphatase-Catalyzed Chemiluminescence

Hongwei Yuan, Haolin Lu, Wei Wan, Xin Jiang et autres

Owing to target recognition hindrance, common metal–organic frameworks (MOFs) as nanocarriers lead to the dramatically decreased catalytic activity (by 90%) of immobilized enzymes. Herein, a dopamine-modulated enclosure strategy was proposed to successfully immobilize alkaline phosphatase (ALP) on phosphatase-like Ce-2-methylimidazole (2-MIm) MOFs for …

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Immunoassays or pseudo-immunoassay for clinical therapeutic drug monitoring applications

Yong He, Fei Ying Yang, Yunxia Li, Lulu Wu et autres

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Plasmonic Nanozyme-Driven Chemiluminescence, Temperature, and RGB Multimodal Sensing for Reliable Discrimination of Enantiomers

Ruining Bai, Xinyue Gao, Jiaying Zhou, Peng Jin et autres

Multimodal probes can output multidimensional signals and meet the demand for discriminating highly similar enantiomers. Their diverse signal transducing mechanisms broaden the discrimination scope of enantiomers and ensure adaptability in various application scenarios. Herein, an achiral multimodal probe termed gold-cobalt nanoparticles (AuCoNPs) …

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Valve-controlled needle-type “mix and detect” integrated distance-based sensor with visual color zoning for point-of-care testing of tacrolimus

Lulu Wu, Fei Yang, Yuchan Ma, Li Tang et autres

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A Recyclable Affinity Chromatographic Device for Rapid Colorimetry of Bacteria Using Phage-Derived Cell Wall Binding Domains As Recognition Ligands

Chong Yu, Honglin Yang, Hongwei Yuan, Ting Guo et autres

Traditional methods for detecting bacteria are usually limited by low efficiency, high cost, and resource dependency. This study developed a recyclable affinity chromatographic device using phage-derived cell wall binding domains (CBDs) as recognition ligands for point-of-care testing of bacteria. The device trapped …

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Pyrene-doped ZIF-8 possesses high fluorescent quantum yield for sensitive ratiometric immunoassay

Wenwen Wang, Xin Jiang, Junyi Huang, Yuchan Ma et autres

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