Tohru Minamino

Thermal fluctuations assist high-speed rotation of the bacterial flagellar motor at low load

Shuichi Nakamura, Yusuke V. Morimoto, Nobunori Kami‐ike, Tohru Minamino et autres

Abstract The bacterial flagellar motor is a proton-driven rotary nanomachine that converts ion flow into mechanical motion. Proton-coupled rotation of the MotA 5 -MotB 2 stator complex generates torque, yet how this process drives high-speed rotation remains unclear. Here we use single-molecule …

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Structural characterization of a pseudaminic acid-modified lateral flagellar filament from Vibrio alginolyticus

Miki Kinoshita, Tohru Minamino, Hajime Nakatani, Atsuo Suzuki et autres

ABSTRACT Bacterial flagellar filaments are often modified by glycans, but the structural basis and physiological significance of flagellin glycosylation remain poorly understood in many species. Vibrio alginolyticus produces lateral flagella for surface-associated motility in viscous environments, with LafA forming its filament as …

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Dynamic topological remodeling underlies assembly of the flagellar protein-export channel FliPQR complex

Miki Kinoshita, Akihiro Kawamoto, Keiichi Namba, Tohru Minamino

FliP, FliQ, and FliR form the membrane-embedded protein-export channel that powers bacterial flagellar assembly. How they assemble into a functional export channel remains unclear. Here we combine topological mapping, cryo-EM image analysis, and biochemical assays to investigate the assembly mechanism of the …

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Structural insights into the assembly mechanism of the molecular bushing in the bacterial flagellar motor

Tomoko Yamaguchi, Tohru Minamino, Takayuki Kato, Fabienne F. V. Chevance et autres

The LP-ring complex of the Salmonella flagellar motor acts as a bushing that supports high-speed rotation of the rod, which serves as a drive shaft. The L-ring, P-ring, and distal rod consist of FlgH, FlgI, and FlgG, respectively. LP-ring formation begins with …

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26-CCC-8648-ACC INCIDENTAL DETECTION OF SILENT MYOCARDIAL ISCHEMIA DURING FDG-PET SURVEILLANCE FOR ESOPHAGEAL CANCER: AN ONCO-CARDIOLOGY PERSPECTIVE

Azusa Shimabukuro, Tadao Aikawa, Takao Kato, Tohru Minamino

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Active removal of inhibitory components drives the flagellar Type III Secretion Specificity Switch

Fabienne F. V. Chevance, Dara Niketic, Di Wu, Carl Ty Mellor et autres

Abstract Type III secretion (T3S) systems assemble bacterial nanomachines, including the flagellum and virulence-associated injectisomes, by exporting distinct classes of substrates in a defined temporal order. In both systems, completion of an early assembly intermediate triggers an irreversible switch from early to …

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Sex Differences in Antiarrhythmic Effects of Empagliflozin

Miyo Nakano, Yusuke Kondo, Shinya Fujiki, Yuki Shiko et autres

BACKGROUND: The EMPA-ICD (Empagliflozin in Patients with Type 2 Diabetes Treated with an Implantable Cardioverter-Defibrillator; jRCTs031180120) trial is a prospective, multicenter, randomized, double-blind, placebo-controlled study evaluating the effects of empagliflozin on ventricular arrhythmias (VAs) in patients with type 2 diabetes (T2DM) treated …

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Comparative Prognostic Value of Four Urinary Biomarkers for Cardiovascular and Renal Outcomes after Transcatheter Aortic Valve Implantation

Hidetoshi Yasuda, Hiroshi Iwata, Shinichiro Doi, Takuma Koike et autres

Introduction: Renal risk stratification after transcatheter aortic valve implantation (TAVI) remains anchored to filtration metrics such as serum creatinine and estimated glomerular filtration rate (eGFR). These indices may not capture early tubular injury, which is clinically relevant in older TAVI populations. We …

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Structural basis for substrate specificity switching revealed by the FlhA(A489E) suppressor mutation

Motoshi Sakai, Miki Kinoshita, Norihiro Takekawa, Keiichi Namba et autres

The flagellar type III secretion system (fT3SS) switches its substrate specificity from hook-type to filament-type upon completion of hook assembly. This process is regulated by the coordinated actions of FliK, FlhB, and FlhA, but the structural mechanism underlying substrate specificity switching remains …

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Reversible Left Ventricular Dysfunction Related to Ropeginterferon Alfa-2b in Polycythemia Vera

Tadao Aikawa, Shuichi Shirane, Tohru Minamino

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Determinants and prognostic impact of left ventricular remodeling in patients with Atrial Functional Mitral Regurgitation: a subanalysis from the REVEAL-AFMR

Akihiro Sakamoto, Takeshi Kaneko, Masashi Amano, Yukio Sato et autres

Abstract Background Atrial functional mitral regurgitation (AFMR) has been reported to have a small and stiff left ventricle and occasionally coexists with concentric left ventricular hypertrophy (LVH), while degenerative mitral regurgitation typically leads to eccentric LVH due to volume overload. The impact …

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Prognostic factors and outcomes in patients under 75 years old receiving transcatheter aortic valve implantation: insights from the LAPLACE registry

Ryotaro Matsuo, S Doi, N Takahashi, H Iwata et autres

Abstract Background Transcatheter aortic valve implantation (TAVI) is an established treatment for severe aortic stenosis. However, from the perspective of prosthetic valve durability, surgical aortic valve replacement (SAVR) is often prioritized in younger patients. The ESC/EACTS Guidelines for the management of valvular …

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