Douglas B. Kell

The Gini coefficient as a surrogate for the regulability or homeostasis of metabolite concentrations: focus on ergothioneine

Douglas B. Kell, Warwick B. Dunn, Catherine Winder, Keerthana Anand et autres

The Gini coefficient is a simple non-parametric statistic that reflects the degree of variation of a property between individuals or samples, taking a value between zero and one. We here develop the idea that if the metabolism of a metabolite is tightly …

gb, Afrique du Sud, Ouganda (code pays fourni par la source)

Persistent Hypercoagulability and Further Characterization of Microclot Complexes in Long COVID

Massimo Nunes, Carlos M Pereira Guerreiro, Jan H Pretorius, Chantelle Venter et autres

Abstract Background Growing evidence suggests persistent thrombotic endothelial damage (together with elevated (fibrinaloid) microclot complexes (FMCs)) and immune dysfunction in the pathophysiology of Long COVID. Recently we proposed that there are different FMC phenotypes. Here we seek to determine the nature of …

Afrique du Sud, fr, gb (code pays fourni par la source)

Atherosclerosis as a Localised Amyloid and Proteostasis Disorder

Douglas B. Kell, Etheresia Pretorius

Atherosclerosis and atherosclerotic plaque formation underpin a variety of acute cardiovascular events including myocardial infarction and ischaemic stroke, and vascular diseases of this type are effectively the leading causes of death worldwide. Despite the presence of many proteases that are normally capable …

Unlocking substrate specificities of human solute carrier proteins using untargeted metabolomics

Yuesheng Zhang, Lyubomir Dimitrov Stanchev, Felicia Cara Schulz, Daniela Rago et autres

Abstract The limited understanding of transporter substrate spectra constrains our ability to interpret cell and membrane function, highlighting the need for methods that enable transporter deorphanization and characterization of promiscuous transport activities. Here, we present a Xenopus oocyte-based platform for unbiased transporter …

dk, gb, Afrique du Sud (code pays fourni par la source)

Post-translational modifications in the insoluble fraction of platelet-poor plasma distinguish pre-COVID-19 Postural Orthostatic Tachycardia Syndrome, Long COVID, and Long COVID-POTS: a preliminary study

Renata M. Booyens, Maré Vlok, Carien Bester, Rashmin Hira et autres

Pre-COVID-19 Postural Orthostatic Tachycardia Syndrome (PC-POTS), Long COVID with initial orthostatic hypotension (LC-IOH), and Long COVID-POTS (LC-POTS) are primarily chronic post-viral conditions with debilitating symptoms, despite routine laboratory findings often being normal. We previously identified amyloidogenic fibrinaloid microclot complexes (FMCs) in the …

Afrique du Sud, ca, gb (code pays fourni par la source)

Advances, challenges, and opportunities in engineering C5 and C6 sugar transporters in yeast for bio-based industrial biotechnology

João Gabriel Ribeiro Bueno, Ícaro Fier, Douglas B. Kell, Leandro Vieira dos Santos

Harnessing biomass for bio-based industrial biotechnology is vital for addressing global energy needs and mitigating climate change. In this context, microorganisms are the cornerstone of biorefineries based on renewable materials, with applications in bioenergy, agriculture, biomedicine, and other sectors. By engineering metabolic …

Advances, challenges, and opportunities in engineering C5 and C6 sugar transporters in yeast for bio-based industrial biotechnology

João Gabriel Ribeiro Bueno, Ícaro Fier, Douglas B. Kell, Leandro Vieira dos Santos

Harnessing biomass for bio-based industrial biotechnology is vital for addressing global energy needs and mitigating climate change. In this context, microorganisms are the cornerstone of biorefineries based on renewable materials, with applications in bioenergy, agriculture, biomedicine, and other sectors. By engineering metabolic …

Advances, challenges, and opportunities in engineering C5 and C6 sugar transporters in yeast for bio-based industrial biotechnology

João Gabriel Ribeiro Bueno, Ícaro Fier, Douglas B. Kell, Leandro Vieira dos Santos

Harnessing biomass for bio-based industrial biotechnology is vital for addressing global energy needs and mitigating climate change. In this context, microorganisms are the cornerstone of biorefineries based on renewable materials, with applications in bioenergy, agriculture, biomedicine, and other sectors. By engineering metabolic …

br, gb, dk, cz (code pays fourni par la source)

The Gini coefficient as a surrogate for the regulability or homeostasis of metabolite concentrations: focus on ergothioneine

Douglas B. Kell, Warwick B. Dunn, Catherine Winder, Keerthana Anand et autres

gb (code pays fourni par la source)

Bioinformatic assessment of the potential amyloidogenicity of the human and evolutionarily more ancient proteomes

Ivayla Roberts, Xiaomian Tan, J. Bernadette Moore, Douglas B. Kell

AmyloGram is a computer program that uses n-gram encoding and a random forest classifier to produce a numerical score between 0 and 1 for the predicted amyloidogenicity of a given protein sequence. In a variety of recent studies, we have used AmyloGram …

gb, Afrique du Sud, dk (code pays fourni par la source)

Surface cues shape procoagulant properties of amyloidogenic microclots

Etheresia Pretorius, Chantelle Venter, Massimo Nunes, Alain R. Thierry et autres

Hypercoagulability, immunothrombosis, and protein misfolding are deeply interconnected processes that converge on cell membranes as central orchestrators of thrombo-inflammation. In health, membrane lipid asymmetry, intact glycocalyx, and regulated receptor activity maintain vascular homeostasis. During inflammation or cell death, however, phosphatidylserine (PS) externalization, …

gb, Afrique du Sud, fr (code pays fourni par la source)

Why Standard Tests Miss Spike-Induced Clot Alterations

Etheresia Pretorius, Douglas B. Kell

Afrique du Sud, gb (code pays fourni par la source)