Edson Yu Sin Kim

Characterization of new thermophilic antibiotic resistance markers

Fernanda Souza Lopes, Renato Vicentini, Edson Yu Sin Kim, Nandhini Ashok et autres

Abstract The genetic engineering of thermophilic bacteria is constrained by limited availability of thermostable antibiotic resistance markers for selection. Clostridium thermocellum , a promising candidate for consolidated bioprocessing of lignocellulosic biomass, requires reliable selection systems that function at elevated temperatures. Here, we …

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Optimisation of DNA electroporation protocols for different plant-associated bacteria

Edson Yu Sin Kim, Emanuel Maltempi de Souza, Marcelo Müller‐Santos

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Improved protocol for Bst polymerase and reverse transcriptase production and application to a point-of-care diagnostics system

Lucas R. de Souza, Ítalo Esposti Poly da Silva, Gabriele Celis-Silva, Bruna Winkert Raddatz et autres

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has raised awareness in the scientific community about the importance of being prepared for sanitary emergencies. Many measures implemented during the COVID pandemic are now being expanded to other applications. In …

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Data treatment methods for real-time colorimetric loop-mediated isothermal amplification reactions

Edson Yu Sin Kim, Louise Matiê Imamura, Bruna Winkert Raddatz, Santiago Pedro Timm Soares et autres

With the SARS-CoV-2 pandemic and the need for affordable and rapid mass testing, colorimetric isothermal amplification reactions such as Loop-Mediated Isothermal Amplification (LAMP) are quickly rising in importance. The technique generates data that is similar to quantitative Polymerase Chain Reaction (qPCR), but …

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Optimisation of DNA Electroporation Protocols for Different Plant-Associated Bacteria

Edson Yu Sin Kim, Emanuel Maltempi de Souza, Marcelo Müller‐Santos

Development of an optimized colorimetric RT-LAMP for SARS-CoV-2 assay with enhanced procedure controls for remote diagnostics

Bruna Winkert Raddatz, Edson Yu Sin Kim, Louise Matiê Imamura, Gisleine Jarenko Steil et autres

The coronavirus pandemic accentuated the need for molecular diagnostic tests. A technique highly used to this end is the Polymerase Chain Reaction (PCR)-a sensitive and specific technique commonly used as the gold standard for molecular diagnostics. However, it demands highly trained personnel …

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Author Correction: The transcriptional regulator NtrC controls glucose-6-phosphate dehydrogenase expression and polyhydroxybutyrate synthesis through NADPH availability in Herbaspirillum seropedicae

Euclides Nenga Manuel Sacomboio, Edson Yu Sin Kim, Henrique Leonardo Ruchaud Correa, Paloma Bonato et autres

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

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The transcriptional regulator NtrC controls glucose-6-phosphate dehydrogenase expression and polyhydroxybutyrate synthesis through NADPH availability in Herbaspirillum seropedicae

Euclides Nenga Manuel Sacomboio, Edson Yu Sin Kim, Henrique Leonardo Ruchaud Correa, Paloma Bonato et autres

Abstract The NTR system is the major regulator of nitrogen metabolism in Bacteria. Despite its broad and well-known role in the assimilation, biosynthesis and recycling of nitrogenous molecules, little is known about its role in carbon metabolism. In this work, we present …

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Aplicação de ferramentas de engenharia metabólica em Herbaspirillum seropedicae Smr1 para aumento na produção de polihidroxibutirato

Edson Yu Sin Kim

Orientador : Prof. Dr. Marcelo Müller dos Santos