Yingqing Huo

Clusterin maintains hemostatic equilibrium by stabilizing VWF multimers in plasma

Ziqi Qiao, Yang Cao, Jingge Su, Shan Lu et autres

ABSTRACT: The coagulation-anticoagulation balance is tightly regulated by endothelial-derived factors, which have not been clearly defined. Here, we report that clusterin, a component of Weibel-Palade bodies (WPBs), plays a crucial role in maintaining hemostatic equilibrium by stabilizing von Willebrand factor (VWF) multimers …

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Defective VWF secretion due to expression ofMYH9-RD E1841K mutant in endothelial cells disrupts hemostasis

Yang Cao, Yanjie Sun, Yanan Deng, Guoqin Wei et autres

Mutations in MYH9, the gene encoding the heavy chain of nonmuscle myosin IIa (NMII-A), cause MYH9-related disease (MYH9-RD), which is an autosomal-dominant thrombocytopenia with bleeding tendency. Previously, we showed that NMII-A in endothelial cells (ECs) is critical for hemostasis via regulating von …

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Meningeal lymphatics regulate radiotherapy efficacy through modulating anti-tumor immunity

Changping Zhou, Lu Ma, Han Xu, Yingqing Huo et autres

Abstract As a first-line treatment, radiotherapy (RT) is known to modulate the immune microenvironment of glioma, but it is unknown whether the meningeal lymphatic vessel (MLV)-cervical lymph node (CLN) network regulates the process or influences RT efficacy. Here, we show that the …

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Zyxin Mediates Vascular Repair via Endothelial Migration Promoted by Forskolin in Mice

Xuya Kang, Yanan Deng, Yang Cao, Yingqing Huo et autres

Background and Purpose: Endothelial repair upon vascular injury is critical for the protection of vessel integrity and prevention of the development of vascular disorders, but the underlying mechanisms remain poorly understood. In this study, we investigated the role of zyxin and its …

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Meningeal lymphatic vessels regulate brain tumor drainage and immunity

Xueting Hu, Qiuping Deng, Lu Ma, Qingqing Li et autres

Recent studies have shown that meningeal lymphatic vessels (MLVs), which are located both dorsally and basally beneath the skull, provide a route for draining macromolecules and trafficking immune cells from the central nervous system (CNS) into cervical lymph nodes (CLNs), and thus …

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Myosin IIa is critical for cAMP-mediated endothelial secretion of von Willebrand factor

Pin Li, Guoqin Wei, Yang Cao, Qiuping Deng et autres

Nonmuscle myosin II has been implicated in regulation of von Willebrand factor (VWF) release from endothelial Weibel-Palade bodies (WPBs), but the specific role of myosin IIa isoform is poorly defined. Here, we report that myosin IIa is expressed both in primary human …

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Zyxin regulates endothelial von Willebrand factor secretion by reorganizing actin filaments around exocytic granules

Xiaofan Han, Pin Li, Zhenghao Yang, Xiaoshuai Huang et autres

Endothelial exocytosis of Weibel-Palade body (WPB) is one of the first lines of defence against vascular injury. However, the mechanisms that control WPB exocytosis in the final stages (including the docking, priming and fusion of granules) are poorly understood. Here we show …

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Endothelial Gab1 deficiency aggravates splenomegaly in portal hypertension independent of angiogenesis

Beibei Jiang, Qiuping Deng, Yingqing Huo, Wěi Li et autres

Certain pathological changes, including angiogenesis, actively contribute to the pathogenesis of splenomegaly in portal hypertension (PH), although the detailed molecular and cellular mechanisms remain elusive. In this study, we demonstrated that endothelial Grb-2-associated binder 1 (Gab1) plays a negative role in PH-associated …

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Endothelial mechanosensors: the gatekeepers of vascular homeostasis and adaptation under mechanical stress

Qiuping Deng, Yingqing Huo, Jincai Luo

Endothelial cells (ECs) not only serve as a barrier between blood and extravascular space to modulate the exchange of fluid, macromolecules and cells, but also play a critical role in regulation of vascular homeostasis and adaptation under mechanical stimulus via intrinsic mechanotransduction. …

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[Cell surface adhesion molecules mediated stretch-induced HL-60 cell adhesion to mouse common carotid arteries].

Yingqing Huo, Beibei Jiang, Xiaofan Han, Jincai Luo

OBJECTIVE: To compare the roles of vascular cell adhesion molecular-1 (VCAM-1) and P-selectin in stretch-induced HL-60 cell adhesion to mouse common carotid arteries. METHODS: After the isolated mouse common carotid arteries were perfused with HL-60 cells, the unbound leukocytes were washed away …

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[An effective method of isolation and primary culture of human umbilical arterial endothelial cells].

Yingqing Huo, Lei Wang, Shujun He, Xiaofan Han et autres

OBJECTIVE: To explore and establish an efficient system for isolation and culture of human umbilical arterial endothelial cells (HUAECs). METHODS: The human umbilical arteries were perfused and washed by PBS, and then digested by 0.1 g/L type 1 collagenase. Digested cell supernatants …

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Grb2-associated binder 1 is essential for cardioprotection against ischemia/reperfusion injury

Lulu Sun, Chao Chen, Beibei Jiang, Yanli Li et autres

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