Maarten Boon

Mining Phage Genomes for Regulatory Elements to Develop a SynBio Toolbox for Non-Model Pseudomonads

Jorien Poppeliers, Maarten Boon, Alison Kerremens, Rob Lavigne

Abstract Bacteriophages represent a largely untapped reservoir of regulatory elements that can expand the genetic toolbox for engineering non-model hosts. Using ONT-cappable-sequencing, we mapped full-length primary transcripts across five Pseudomonas phages and identified 232 transcription start sites (TSSs) and 176 transcription termination …

be (code pays fourni par la source)

High-resolution transcriptome analysis reveals a multilayered and dynamic transcriptional architecture in the archaeal virus SSV1

Wouter Magnus, Maarten Boon, Jorien Poppeliers, Jonathan Abshier et autres

Abstract Fuselloviridae infecting thermoacidophilic Sulfolobales are among the best-studied archaeal viruses, with the Saccharolobus Spindle Shaped Virus 1 (SSV1)- Saccharolobus solfataricus interaction serving as a model system. After infection, SSV1 establishes a chronic infection with new virions continuously budding from the infected …

be, us (code pays fourni par la source)

Research data supporting "High-resolution transcriptome analysis reveals a multilayered and dynamic transcriptional architecture in the archaeal virus SSV1"

Wouter Magnus, Eveline Peeters, Jorien Poppeliers, Maarten Boon et autres

This dataset contains the raw research data supporting the manuscript "High-resolution transcriptome analysis reveals a multilayered and dynamic transcriptional architecture in the archaeal virus SSV1". The accompanying README.txt file describes what specific data can be found in which file.

be (code pays fourni par la source)

Research data supporting "High-resolution transcriptome analysis reveals a multilayered and dynamic transcriptional architecture in the archaeal virus SSV1"

Wouter Magnus, Eveline Peeters, Jorien Poppeliers, Maarten Boon et autres

This dataset contains the raw research data supporting the manuscript "High-resolution transcriptome analysis reveals a multilayered and dynamic transcriptional architecture in the archaeal virus SSV1". The accompanying README.txt file describes what specific data can be found in which file.

be (code pays fourni par la source)

Fluorescence-Based Assays to Evaluate the Functionality of Phage-Derived Regulatory Elements for Applications in Synthetic Biology

Jorien Poppeliers, Alison Kerremans, Riet Dehaene, Maarten Boon et autres

be (code pays fourni par la source)

High-Level Protein Expression in Pseudomonas putida Using the Phage-Based pPUT Expression System

Riet Dehaene, Jorien Poppeliers, Rob Lavigne, Maarten Boon

be (code pays fourni par la source)

Non-model bacteria as platforms for endogenous gene expression in synthetic biology

Jorien Poppeliers, Maarten Boon, Marjan De Mey, Joleen Masschelein et autres

be (code pays fourni par la source)

Multi-omics characterization of a lytic phage targeting Listeria monocytogenes

Stevan Cucić, Leena Putzeys, Maarten Boon, Dion Lepp et autres

ABSTRACT Listeria monocytogenes is a foodborne pathogenic bacterium that can persist in food-processing environments. Strictly lytic Listeria phages have shown promise as biosanitation and biocontrol agents. However, little is known about the molecular progression of phage expression and the host gene expression …

ca, fr, be (code pays fourni par la source)

Phage therapy

Mikael Skurnik, Sivan Alkalay‐Oren, Maarten Boon, Martha R. J. Clokie et autres

fi, il, be, gb, dk, my, pl, us, ch (code pays fourni par la source)

Engineering a phi15-based expression system for stringent gene expression in Pseudomonas putida

Eveline‐Marie Lammens, Daniel Christophe Volke, Alison Kerremans, Yannick Aerts et autres

The T7 phage RNA polymerase (RNAP) is a widely used expression platform, but its implementation in non-model microbial hosts poses significant challenges due to cytotoxicity. We constructed an optimized phage phi15-based expression system as alternative to the T7 platform for a wide …

be, dk (code pays fourni par la source)

PlzR regulates type IV pili assembly in Pseudomonas aeruginosa via PilZ binding

Hanne Hendrix, Annabel Itterbeek, Hannelore Longin, Lize Delanghe et autres

Type IV pili (T4P) are thin, flexible filaments exposed on the cell surface of gram-negative bacteria and are involved in pathogenesis-related processes, including cell adsorption, biofilm formation, and twitching motility. Bacteriophages often use these filaments as receptors to infect host cells. Here, …

be, it, us, nl (code pays fourni par la source)

Assessing the transcriptional landscape of Pseudomonas phage 201ϕ2‐1: Uncovering the small regulatory details of a giant phage

Jorien Poppeliers, Mathijs Focquet, Maarten Boon, Marjan De Mey et autres

The transcriptional architecture of phages can deepen our understanding of the phage-host infection process and can be of key importance for phage engineering and biotechnological applications. Here, we applied ONT-cappable-sequencing, a long-read RNA-sequencing technique, to study the regulatory mechanisms of Pseudomonas infecting …

be, us (code pays fourni par la source)