Carmella van de Ven

Genomic and Immunomic Characterization, Safety and Efficacy of Related Donor-Derived Cytotoxic T-Lymphocytes (CTLs) in Immunocompromised Recipients with Medically Refractory Adv and CMV Infection: On Behalf of the Viral CTL Consortium (VIRCTLC)

Mitchell S. Cairo, Dean Anthony Lee, Laura J. Vella, Yanling Liao et autres

Neurocognitive outcome in children with sickle cell disease after myeloimmunoablative conditioning and haploidentical hematopoietic stem cell transplantation: a non-randomized clinical trial

Suzanne H. Braniecki, Elliott P. Vichinsky, Mark C. Walters, Shalini S. Shenoy et autres

Background Due to the risk of cerebral vascular injury, children and adolescents with high-risk sickle cell disease (SCD) experience neurocognitive decline over time. Haploidentical stem cell transplantation (HISCT) from human leukocyte antigen-matched sibling donors may slow or stop progression of neurocognitive changes. …

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Adenoviral Cellular Therapy with Adenovirus (ADV)-Specific Cytotoxic T-Lymphocytes (CTLs) for Resistant Infections in Children, Adolescent, and Young Adult (CAYA) Patients Following Allogeneic Hematopoietic Stem Cell Transplantation (AlloHSCT): A Promising Approach

Edo Schaefer, Christine A. Vu, Allyson M. Flower, Janet Ayello et autres

AlloHSCT serves as a long-term curative intervention for CAYA with varied disorders but poses risks like refractory ADV infections due to impaired T-cell immunity (George/Cairo, BJH, 2012). Here, we report data from the Viral Cytotoxic T-Lymphocyte Consortium (VIRCTLC), showing the safety and …

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Treatment of Refractory Adenovirus (ADV) Infections Using Adv-Specific Cytotoxic T-Lymphocytes (CTLs) in Children, Adolescents and Young Adults (CAYA) Post Allogeneic Hematopoietic Stem Cell Transplantation (AlloHSCT), Solid Organ Transplantation (SOT), or with Primary Immunodeficiency (PID)

Edo Schaefer, Christine A. Vu, Allyson M. Flower, Janet Ayello et autres

Background: AlloHSCT induces long-term, cure in CAYA with malignant and non-malignant disorders, including those with primary immunodeficiencies (PID). However, post-transplant complications secondary to immunosuppression and slow or delayed immune reconstitution remains a cause of morbidity and mortality amongst AlloHSCT recipients (Satwani/Cairo, BBMT, …

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Risk Factors Associated with Survival Following Ganciclovir Prophylaxis through Day +100 in Cytomegalovirus At-Risk Pediatric Allogeneic Stem Cell Transplantation Recipients: Development of Cytomegalovirus Viremia Associated with Significantly Decreased 1-Year Survival

Liana M. Klejmont, Xiaokui Molly Mo, Jordan Milner, Lauren A. Harrison et autres

Cytomegalovirus (CMV) reactivation is a major cause of morbidity and nonrelapse mortality (NRM) in pediatric allogeneic stem cell transplantation (alloSCT) recipients. Approximately 80% of CMV seropositive alloHCT recipients will experience CMV reactivation without prophylaxis. The impacts of ganciclovir prophylaxis and subsequent CMV …

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Supplementary Figure S6 from Targeting CD20+ Aggressive B-cell Non–Hodgkin Lymphoma by Anti-CD20 CAR mRNA-Modified Expanded Natural Killer Cells In Vitro and in NSG Mice

Yaya Chu, Jessica C. Hochberg, Ashlin Yahr, Janet Ayello et autres

Supplementary Fig.S6: The purified expanded PBNK cells have higher activating receptors expression PBNK cells were expanded ex vivo by co-culturing PBMC with irradiated geK562 feeder cell lines in the presence of 40 U/mL IL-2 for 14 days and isolated by depleting CD3+ …

Supplementary Figure S2 from Targeting CD20+ Aggressive B-cell Non–Hodgkin Lymphoma by Anti-CD20 CAR mRNA-Modified Expanded Natural Killer Cells In Vitro and in NSG Mice

Yaya Chu, Jessica C. Hochberg, Ashlin Yahr, Janet Ayello et autres

Supplementary Fig.S2: nucleofection of anti-CD20 CAR mRNA does not affect the expression of endogenous receptors on exPBNK cells PBNK cells were expanded ex vivo by co-culturing PBMC with irradiated geK562 feeder cell lines in the presence of 40 IU/mL IL-2 for 14 …

Supplementary Figure S3 from Targeting CD20+ Aggressive B-cell Non–Hodgkin Lymphoma by Anti-CD20 CAR mRNA-Modified Expanded Natural Killer Cells In Vitro and in NSG Mice

Yaya Chu, Jessica C. Hochberg, Ashlin Yahr, Janet Ayello et autres

Supplementary Fig.S3: U-698-M stimulates the expression of activating receptors on mock exPBNK and CAR exPBNK similarly The expanded NK cells were nucleofected with anti-CD20 CAR mRNA (CAR) or without anti-CD20 CAR mRNA (mock). MOCK exPBNK or CAR exPBNK cells were cultured with …

Supplementary Figure S4 from Targeting CD20+ Aggressive B-cell Non–Hodgkin Lymphoma by Anti-CD20 CAR mRNA-Modified Expanded Natural Killer Cells In Vitro and in NSG Mice

Yaya Chu, Jessica C. Hochberg, Ashlin Yahr, Janet Ayello et autres

Supplementary Fig.S4: In vitro bioluminescence to detect luciferase expression in Raji and Raji-2R cells Raji and Raji-2R cells were electroporated with ffLUCZeo-pcDNA and selected for stable clones expressing luciferase (Luc) with Zeocin. (A) The bioluminescence intensity increased proportionally with increasing Raji-Luc cell …

Supplementary Figure S1 from Targeting CD20+ Aggressive B-cell Non–Hodgkin Lymphoma by Anti-CD20 CAR mRNA-Modified Expanded Natural Killer Cells In Vitro and in NSG Mice

Yaya Chu, Jessica C. Hochberg, Ashlin Yahr, Janet Ayello et autres

Supplementary Fig.S1: Anti-CD20 CAR enhances NK92 cells in-vitro cytolytic activity against CD20+ B-NHL cells by retroviral transduction. (A) Schematic representation of the anti-CD20 chimeric receptor in an RD114-pseudotyped retroviral vector. LTR indicates long terminal repeat; AMP, ampicillin resistance. (B)-(C) Expression of anti-CD20 …

Supplementary Figure S5 from Targeting CD20+ Aggressive B-cell Non–Hodgkin Lymphoma by Anti-CD20 CAR mRNA-Modified Expanded Natural Killer Cells In Vitro and in NSG Mice

Yaya Chu, Jessica C. Hochberg, Ashlin Yahr, Janet Ayello et autres

Supplementary Fig.S5: exPBNK cells tracking and persistence in vivo (A) exPBNK cells were labeled with DID and iv injected into NSG mice. 8 days later after NK injection, mice were sacrificed and organs (lung, liver, spleen, kidney, heart) and bones were collected …

Supplementary Figure S7 from Targeting CD20+ Aggressive B-cell Non–Hodgkin Lymphoma by Anti-CD20 CAR mRNA-Modified Expanded Natural Killer Cells In Vitro and in NSG Mice

Yaya Chu, Jessica C. Hochberg, Ashlin Yahr, Janet Ayello et autres

Supplementary Fig.S7: Proposed scientific model We propose that the interaction between anti-CD20 CAR on exPBNK and CD20 on the surface of B-L/L transmits activating signal back to exPBNK and significantly stimulates exPBNK degranulation and release of cytokine such as IFN-y to lyse …