Devin Dersh

T-cell immunosenescence limits CD19 CAR T-cell function in chronic lymphocytic leukemia

Julia Han Noll, Devin Dersh, Jiang Yuan Li, Humza Hemani et autres

CD19-directed CAR T-cells (CTL019) can produce durable remissions in chronic lymphocytic leukemia (CLL), but therapeutic success depends on whether autologous T-cells expand, persist, and retain cytotoxic function after manufacturing. Failure of CLL T-cells is often attributed to exhaustion, although many dysfunctional CLL …

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A modular γδ TCR-T platform combining KRAS pMHC targeting with re-dosable mRNA engager redirection

Ángel Ramírez-Fernández, Adham S. Bear, Elahe Kamali, Robert Bartoszek et autres

Solid tumors often evade TCR-engineered αβ T cells when antigen expression varies or when the restricting Human Leukocyte Antigen (HLA) allele is lost. γδ T cells, in contrast, detect cellular dysregulation through non-peptide/Major Histocompatibility Complex (MHC) cues, including phosphoantigens and stress ligands, …

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Targeting peptide antigens using a multiallelic MHC I-binding system

Haotian Du, Leena Mallik, Daniel Hwang, Yi Sun et autres

Identifying highly specific T cell receptors (TCRs) or antibodies against epitopic peptides presented by class I major histocompatibility complex (MHC I) proteins remains a bottleneck in the development of targeted therapeutics. Here, we introduce targeted recognition of antigen-MHC complex reporter for MHC …

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Flow-based In Vivo Method to Enumerate Translating Ribosomes and Translation Elongation Rate

Mina O. Seedhom, Devin Dersh, Jonathan W. Yewdell

Protein synthesis is by far the most energetically costly cellular process in rapidly dividing cells. Quantifying translating ribosomes in individual cells and their average mRNA transit rate is arduous. Quantitating assembled ribosomes in individual cells requires electron microscopy and does not indicate …

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Author Response: Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate

Mina O. Seedhom, Devin Dersh, Jaroslav Hollý, Mariana Pavon-Eternod et autres

Quantitative protein and ribosome 'accounting' in T cells highlights a gap in our understanding of how activated T cells sufficiently replicate their proteome during rapid cell division driven by infection.

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Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate

Mina O. Seedhom, Devin Dersh, Jaroslav Hollý, Mariana Pavon-Eternod et autres

Rapid lymphocyte cell division places enormous demands on the protein synthesis machinery. Flow cytometric measurement of puromycylated ribosome-associated nascent chains after treating cells or mice with translation initiation inhibitors reveals that ribosomes in resting lymphocytes in vitro and in vivo elongate at …

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Paradox Found: Global Accounting of Lymphocyte Protein Synthesis

Mina O. Seedhom, Devin Dersh, Jaroslav Hollý, Mariana Pavon-Eternod et autres

Abstract Rapid lymphocyte cell division places enormous demands on the protein synthesis machinery. Flow cytometric measurement of puromycylated ribosome-associated nascent chains after treating cells or mice with translation initiation inhibitors reveals that ribosomes in resting lymphocytes in vitro and in vivo elongate …

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Author Response: Paradox Found: Global Accounting of Lymphocyte Protein Synthesis

Mina O. Seedhom, Devin Dersh, Jaroslav Hollý, Mariana Pavon-Eternod et autres

Rapid lymphocyte cell division places enormous demands on the protein synthesis machinery. Flow cytometric measurement of puromycylated ribosome-associated nascent chains after treating cells or mice with translation initiation inhibitors reveals that ribosomes in resting lymphocytes in vitro and in vivo elongate at …

us, fr (code pays fourni par la source)

Reviewer #3 (Public Review): Paradox Found: Global Accounting of Lymphocyte Protein Synthesis

Mina O. Seedhom, Devin Dersh, Jaroslav Hollý, Mariana Pavon-Eternod et autres

Rapid lymphocyte cell division places enormous demands on the protein synthesis machinery. Flow cytometric measurement of puromycylated ribosome-associated nascent chains after treating cells or mice with translation initiation inhibitors reveals that ribosomes in resting lymphocytes in vitro and in vivo elongate at …

fr, us (code pays fourni par la source)

Reviewer #1 (Public Review): Paradox Found: Global Accounting of Lymphocyte Protein Synthesis

Mina O. Seedhom, Devin Dersh, Jaroslav Hollý, Mariana Pavon-Eternod et autres

Rapid lymphocyte cell division places enormous demands on the protein synthesis machinery. Flow cytometric measurement of puromycylated ribosome-associated nascent chains after treating cells or mice with translation initiation inhibitors reveals that ribosomes in resting lymphocytes in vitro and in vivo elongate at …

fr, us (code pays fourni par la source)

Reviewer #2 (Public Review): Paradox Found: Global Accounting of Lymphocyte Protein Synthesis

Mina O. Seedhom, Devin Dersh, Jaroslav Hollý, Mariana Pavon-Eternod et autres

Rapid lymphocyte cell division places enormous demands on the protein synthesis machinery. Flow cytometric measurement of puromycylated ribosome-associated nascent chains after treating cells or mice with translation initiation inhibitors reveals that ribosomes in resting lymphocytes in vitro and in vivo elongate at …

fr, us (code pays fourni par la source)

SUS(d6)pending MHC class I peptide presentation for cancer immunoevasion

Devin Dersh, Jonathan W. Yewdell

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