Stuart S. Martin

Single-Cell Profiling of Apoptotic Resistance in Patient-Derived Xenografts and Primary Circulating Tumor Cells Using TetherChip

Darin E. Gilchrist, Keyata N. Thompson, Julia A. Ju, David A. Annis et autres

Circulating tumor cells (CTCs) must escape the primary tumor and spread to distant organs through harsh, nonadherent blood, and lymphatic microenvironments, where over 99% perish. Some CTCs survive by resisting extracellular matrix (ECM) detachment-induced apoptosis (anoikis). CTC enumeration has already been directly …

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Melanoma cell adhesion molecule (MCAM) mediates microtentacle generation, homotypic clustering, and reattachment of non-adherent breast tumor cells

David A. Annis, Keyata N. Thompson, Julia A. Ju, Makenzy Mull et autres

BACKGROUND: Melanoma cell adhesion molecule (MCAM) was first identified in advanced primary tumors and metastatic lesions. MCAM expression has been shown in previous studies to promote aggressive cancer phenotypes, including migration, invasion, tumorigenesis, and induction of vimentin and slug, indicative of EMT. …

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Fluorescence Imaging of DMDG-ICG Across NIR-I and NIR-II Windows Using a Single-Camera System

Bonghwan Chon, Mukesh P. Yadav, William Ghann, Stuart S. Martin et autres

Near-infrared (NIR) imaging, including NIR-I (800–1000 nm) and NIR-II (1000–1700 nm), has been primarily evaluated using separate cameras with different detectors, limiting comparison. We investigated whether using a single-camera system capable of both NIR-I and NIR-II acquisition, NIR-II improves spatial resolution and …

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Repurposing Artesunate to Combat Progression and Metastasis via Targeting Circulating Tumor Cells

Evangelia Pantazaka, Dimitrios Papakonstantinou, Argyro Roumeliotou, Dafni G. Graikioti et autres

Objectives: Circulating tumor cells (CTCs) drive metastasis and exhibit resistance to conventional therapies, making them crucial therapeutic targets. Artesunate (AS), a derivative of artemisinin, displays anticancer activity, including inhibition of JunB proto-oncogene (JUNB) and programmed death ligand-1 (PD-L1) and upregulation of Vimentin …

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The Impact of Combretastatin A-4 on Cancer Cells and Circulating Tumor Cells (CTCs): A Multi-Assay Approach

Dimitrios Papakonstantinou, Vasileios Vardas, Despoina M. Varouhaki, Aikaterini Kotzamouratoglou et autres

Objectives: Combretastatin A-4 (CA-4) is a microtubule-disrupting agent with established anti-tumor properties. This study aimed to evaluate the effects of CA-4 on key metastatic traits of cancer cells, including migration, clonogenic potential, cytoskeletal protein expression, and microtentacle (McTN) formation, using multiple cancer …

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Phenotyping and clinical utility of phagocytic polyploid giant cancer macrophages in blood

Daniel Lee Adams, Massimo Cristofanilli, Steven Hsesheng Lin, Raymond C. Bergan et autres

Historically, polyploid giant cancer cells (PGCCs) within tumors have been ignored as superfluous inflammatory refuse with no intrinsic clinical or biological relevance. However recently, multiple studies have described the existence PGCCs in solid tumor masses that appear to correlate with tumor progression, …

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Cytoplasmic anillin and Ect2 promote RhoA/myosin II-dependent confined migration and invasion

Avery Tran, Emily O. Wisniewski, Panagiotis Mistriotis, Konstantin V. Stoletov et autres

Cell migration in mechanically confined environments is a crucial step of metastatic cancer progression. Nonetheless, the molecular components and processes mediating such behaviour are still not fully understood. Here we demonstrate that a pool of the scaffolding protein anillin and its cofactor …

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Disruption of P2Y2 Signaling Promotes Breast Tumor Cell Dissemination by Reducing ATP-Dependent Calcium Elevation and Actin Localization to Cell Junctions

Makenzy L. Mull, Stephen J. P. Pratt, Keyata N. Thompson, David A. Annis et autres

The tumor microenvironment and healing wounds both contain extremely high concentrations of adenosine triphosphate (ATP) compared to normal tissue. The P2Y2 receptor, an ATP-activated purinergic receptor, is typically associated with pulmonary, endothelial, and neurological cell signaling. Here, we examine ATP-dependent signaling in …

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Author Correction: A microfluidic assay for the quantification of the metastatic propensity of breast cancer specimens

Christopher L. Yankaskas, Keyata N. Thompson, Colin D. Paul, Michele I. Vitolo et autres

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Tubulin‐Based Microtentacles Aid in Heterotypic Clustering of Neutrophil‐Differentiated HL‐60 Cells and Breast Tumor Cells

Julia A. Ju, Keyata N. Thompson, David A. Annis, Makenzy L. Mull et autres

Circulating tumor cells (CTCs) travel through the vasculature to seed secondary sites and serve as direct precursors of metastatic outgrowth for many solid tumors. Heterotypic cell clusters form between CTCs and white blood cells (WBCs) and recent studies report that a majority …

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High throughput cell mechanotyping of cell response to cytoskeletal modulations using a microfluidic cell deformation system

Ian Michael Smith, Jeanine A. Ursitti, Sai Pranav Majeti Venkata, Nikka Givpoor et autres

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Stimulated Brillouin scattering flow cytometry

Jake R. Rosvold, Giulia Zanini, Chenchen Handler, Eric Frank et autres

We present the use of stimulated Brillouin scattering spectroscopy to achieve rapid measurements of cell biomechanics in a flow cytometer setup. Specifically, our stimulated Brillouin scattering flow cytometry can acquire at a rate of 200 Hz, with a spectral acquisition time of …

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