Thomas Michna

Prohibitin 2 is a key regulator of T cell proliferation, differentiation, and effector functions in vivo

Johannes F. Vogt, Carsten Merkwirth, Petra Adams‐Quack, Elena Zurkowski et autres

Prohibitin 2 (PHB2) is a highly conserved protein with essential roles in cell homeostasis and survival across different cell types. Previous studies have shown that the deletion of PHB2 results in an arrest in proliferation due to impaired mitochondrial function regulated by …

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OC 49.4 Targeting Myeloid Cell Coagulation Signaling Blocks Map Kinase/TGF-β1 Driven Fibrotic Remodeling, Improves Cardiac Function and Improves Survival in Ischemic Heart Failure

Venkata Garlapati, Michael Molitor, Thomas Michna, Sonja Finger et autres

HowDirty: An R package to evaluate molecular contaminants in LC‐MS experiments

David Gomez‐Zepeda, Thomas Michna, Tanja Ziesmann, Ute Distler et autres

Contaminants derived from consumables, reagents, and sample handling often negatively affect LC-MS data acquisition. In proteomics experiments, they can markedly reduce identification performance, reproducibility, and quantitative robustness. Here, we introduce a data analysis workflow combining MS1 feature extraction in Skyline with HowDirty, …

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Ionmob: a Python package for prediction of peptide collisional cross-section values

David Teschner, David Gomez‐Zepeda, Arthur Declercq, Mateusz Krzysztof Łącki et autres

MOTIVATION: Including ion mobility separation (IMS) into mass spectrometry proteomics experiments is useful to improve coverage and throughput. Many IMS devices enable linking experimentally derived mobility of an ion to its collisional cross-section (CCS), a highly reproducible physicochemical property dependent on the …

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HowDirty: An R package to evaluate molecular contaminants in LC-MS experiments

David Gomez‐Zepeda, Thomas Michna, Tanja Ziesmann, Ute Distler et autres

Contaminants derived from consumables, reagents, and sample handling often negatively affect LC-MS data acquisition. In proteomics experiments, they can markedly reduce identification performance, reproducibility, and quantitative robustness. Here, we introduce a data analysis workflow combining MS1 feature extraction in Skyline with HowDirty, …

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Modulation of cellular transcriptome and proteome composition by azidohomoalanine—implications on click chemistry–based secretome analysis

Friederike Kirschner, Danielle Arnold-Schild, Christian Leps, Mateusz Krzysztof Łącki et autres

The analysis of the secretome provides important information on proteins defining intercellular communication and the recruitment and behavior of cells in specific tissues. Especially in the context of tumors, secretome data can support decisions for diagnosis and therapy. The mass spectrometry-based analysis …

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AKT activity orchestrates marginal zone B cell development in mice and humans

Eva-Maria Cox, Mohamed El Behi, Stefanie Ries, Johannes F. Vogt et autres

The signals controlling marginal zone (MZ) and follicular (FO) B cell development remain incompletely understood. Here, we show that AKT orchestrates MZ B cell formation in mice and humans. Genetic models that increase AKT signaling in B cells or abolish its impact …

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Modulation of cellular transcriptome and proteome composition by azidohomoalanine – implications on click chemistry based secretome analysis

Friederike Kirschner, Danielle Arnold-Schild, Christian Leps, Mateusz Krzysztof Łącki et autres

Abstract The analysis of the secretome provides important information on proteins defining intercellular communication and the recruitment and behavior of cells in specific tissues. Especially in the context of tumors, secretome data can support decisions for diagnosis and therapy. The mass spectrometry-based …

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Data-Independent Acquisition (DIA) Is Superior for High Precision Phospho-Peptide Quantification in Magnaporthe oryzae

Katharina Bersching, Thomas Michna, Stefan Tenzer, Stefan Jacob

The dynamic interplay of signaling networks in most major cellular processes is characterized by the orchestration of reversible protein phosphorylation. Consequently, analytic methods such as quantitative phospho-peptidomics have been pushed forward from a highly specialized edge-technique to a powerful and versatile platform …

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Targeting myeloid cell coagulation signaling blocks MAP kinase/TGF-β1–driven fibrotic remodeling in ischemic heart failure

Venkata Garlapati, Michael Molitor, Thomas Michna, Gregory S. Harms et autres

Despite major advances in acute interventions for myocardial infarction (MI), adverse cardiac remodeling and excess fibrosis after MI causing ischemic heart failure (IHF) remain a leading cause of death worldwide. Here we identify a profibrotic coagulation signaling pathway that can be targeted …

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Data-Independent Acquisition (DIA) is Superior for High Precisely Phospho-Peptide Quantification in Fungi

Katharina Bersching, Thomas Michna, Stefan Tenzer, Stefan Jacob

The dynamic interplay of signaling networks in most major cellular processes is characterized by the orchestration of reversible protein phosphorylation. Consequently, analytic methods like quantitative phospho-peptidomics has been pushed forward from a highly specialized edge-technique to a powerful and versatile platform for …

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Evidence of a New MoYpd1p Phosphotransferase Isoform in the Multistep Phosphorelay System of Magnaporthe oryzae

Sri Bühring, Alexander Yemelin, Thomas Michna, Stefan Tenzer et autres

Different external stimuli are perceived by multiple sensor histidine kinases and transmitted by phosphorylation via the phosphotransfer protein Ypd1p in the multistep phosphorelay system of the high osmolarity glycerol signaling pathway of filamentous fungi. How the signal propagation takes place is still …

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