Accès ouvert
2024
article
OpenAlex
Gianluca Rotta, Valentina Achille, Scott J. Bornheimer, Marco A. Fernández et autres
Flow cytometry characterization of antigen-specific polyfunctional T cells is a valuable tool to study adaptive immunity. Here, we present a protocol for flow cytometry immunophenotyping of human antigen-specific T cells by activation-induced marker (AIM) and Th1 cytokine detection. We describe steps for …
us, it, es, at, gb
(code pays fourni par la source)
Accès ouvert
2023
article
OpenAlex
Tiziana Altosole, Gianluca Rotta, Chiara Uras, Scott J. Bornheimer et autres
Antigen (ag)-specific T cell analysis is an important step for investigation of cellular immunity in many settings, such as infectious diseases, cancer and vaccines. Multiparameter flow cytometry has advantages in studying both the rarity and heterogeneity of these cells. In the cellular …
it, us
(code pays fourni par la source)
Accès ouvert
2022
article
OpenAlex
Cristina Faccani, Gianluca Rotta, Francesca Clemente, Maya Fedeli et autres
We describe a multi-step high-dimensional (HD) flow cytometry workflow for the deep phenotypic characterization of T cells infiltrating metastatic tumor lesions in the liver, particularly derived from colorectal cancer (CRC-LM). First, we applied a novel flow cytometer setting approach based on single …
it
(code pays fourni par la source)
Accès ouvert
2022
preprint
OpenAlex
Tiziana Altosole, Gianluca Rotta, Scott J. Bornheimer, Daniela Fenoglio
ABSTRACT Antigen (ag)-specific T cell analysis is an important step for investigation of cellular immunity in many settings, such as infectious diseases, cancer and vaccines. Multiparameter flow cytometry has advantages in studying both the rarity and heterogeneity of these cells. In the …
it, us
(code pays fourni par la source)
Accès ouvert
2021
article
OpenAlex
Alessandra Dondero, Martina Morini, Davide Cangelosi, Katia Mazzocco et autres
BACKGROUND: High-risk neuroblastomas (HR-NBs) are rare, aggressive pediatric cancers characterized by resistance to therapy and relapse in more than 30% of cases, despite using an aggressive therapeutic protocol including targeting of GD2. The mechanisms responsible for therapy resistance are unclear and might …
it
(code pays fourni par la source)
Accès ouvert
2021
article
OpenAlex
Maria Luisa Martino, Alessandra Frau, Francesca Losa, Emma Muggianu et autres
Endothelial damage and fibro-proliferative vasculopathy of small vessels are pathological hallmarks of systemic sclerosis (SSc). The consequence is the detachment of resident elements that become circulating endothelial cells (CECs). The aim of our study was to evaluate the potential of CECs as …
it
(code pays fourni par la source)
Accès ouvert
2020
article
OpenAlex
Maria Cellerino, Federico Ivaldi, Matteo Pardini, Gianluca Rotta et autres
Objective To establish cytometry profiles associated with disease stages and immunotherapy in MS. Methods Demographic/clinical data and peripheral blood samples were collected from 227 patients with MS and 82 sex- and age-matched healthy controls (HCs) enrolled in a cross-sectional study at 4 …
no, it, es
(code pays fourni par la source)
2019
conference-abstract
OpenAlex
Camillo Almici, Francesca Fontana, Giovanna Piovani, Piera Balzarini et autres
Background. We recently reported that Circulating Endothelial Cell (CEC) count changes represent a promising marker to monitor endothelial damage in patients undergoing allogeneic hematopoietic stem cell transplant (allo-HSCT), potentially becoming a valuable tool in the diagnostic definition of GVHD. Besides confirming an …
it
(code pays fourni par la source)
2019
article
OpenAlex
Cansu Görgün, Daniele Reverberi, Gianluca Rotta, Federico Villa et autres
This unit describes protocols for isolating subpopulations of extracellular vesicles (EVs) purified from human adipose tissue-derived mesenchymal stromal cells by density gradient centrifugation and for characterizing them by flow cytometry (FCM). Determining the optimal strategy for isolating EVs is a critical step …
it, us
(code pays fourni par la source)
Accès ouvert
2019
article
OpenAlex
Camillo Almici, Arabella Neva, Cristina Skert, Benedetto Bruno et autres
Physio-pathologic interrelationships between endothelial layer and graft-versus-host disease (GVHD) have been described leading to assess the entity "endothelial GVHD" as the early step for clinical manifestations of acute GVHD. The availability of the CellSearch system has allowed us to monitor Circulating Endothelial …
it, be
(code pays fourni par la source)
Accès ouvert
2018
article
OpenAlex
Mauro Di Ianni, Stefano Baldoni, Beatrice Del Papa, Patrizia Aureli et autres
To investigate Chronic Lymphocytic Leukemia (CLL)-initiating cells, we assessed NOTCH1 mutation/expression in hematopoietic stem cells (HSCs). In NOTCH1-mutated CLL, we detected subclonal mutations in 57% CD34+/CD38- HSCs. NOTCH1 mutation was present in 66% CD34+/CD38+ progenitor cells displaying an increased mutational burden compared …
it, br
(code pays fourni par la source)
Accès ouvert
2018
article
OpenAlex
Paola Lanuti, Pasquale Simeone, Gianluca Rotta, Camillo Almici et autres
Abstract Circulating endothelial cells (CEC) represent a restricted peripheral blood (PB) cell subpopulation with high potential diagnostic value in many endothelium-involving diseases. However, whereas the interest in CEC studies has grown, the standardization level of their detection has not. Here, we undertook …
it
(code pays fourni par la source)