Jessica S. Sadick

A community-led initiative to de-risk and advance Parkinson’s disease therapeutic targets

Jonathan Behr, Ryan Birol, Cornelis Blauwendraat, Bradford H. Casey et autres

Identifying effective therapeutic targets for Parkinson's disease (PD) is challenging, with no current disease-modifying therapies available. To address this, The Michael J. Fox Foundation for Parkinson's Research launched the Targets to Therapies (T2T) initiative, uniting experts to prioritize and validate promising targets. …

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Multi‐modal sequencing analysis of astrocytes in human and mouse reveals strategically positioned novel reactive sub‐states

Philip Hasel, Michael O’Dea, Jessica S. Sadick, Shane A. Liddelow

Abstract Background Astrocytes can have helpful or harmful effects on neuron health and brain function in disease. While they normally provide trophic support to neurons during development and normal functioning, in response to many stimuli their heterogeneous ‘reactive’ responses can alter these …

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Astrocytes and oligodendrocytes undergo subtype-specific transcriptional changes in Alzheimer’s disease

Jessica S. Sadick, Michael R. O’Dea, Philip Hasel, Taitea Dykstra et autres

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Neuroinflammatory astrocyte subtypes in the mouse brain

Philip Hasel, Indigo V.L. Rose, Jessica S. Sadick, Rachel D. Kim et autres

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CD49f Is a Novel Marker of Functional and Reactive Human iPSC-Derived Astrocytes

Lilianne Barbar, Tanya Jain, Matthew Zimmer, Ilya Kruglikov et autres

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Generating Cell Type-Specific Protein Signatures from Non-symptomatic and Diseased Tissues

Jessica S. Sadick, Lorin A. Crawford, Harry C. Cramer, Christian Franck et autres

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Don't forget astrocytes when targeting Alzheimer's disease

Jessica S. Sadick, Shane A. Liddelow

Astrocytes are essential for CNS health, regulating homeostasis, metabolism, and synaptic transmission. In addition to these and many other physiological roles, the pathological impact of astrocytes ("reactive astrocytes") in acute trauma and chronic disease like Alzheimer's disease (AD) is well established. Growing …

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Cell Mimicking Microparticles Influence the Organization, Growth, and Mechanophenotype of Stem Cell Spheroids

Nicholas R. Labriola, Jessica S. Sadick, Jeffrey R. Morgan, Edith Mathiowitz et autres

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Nuclear Lamin Protein C Is Linked to Lineage-Specific, Whole-Cell Mechanical Properties

Rafael D. González-Cruz, Jessica S. Sadick, Vera C. Fonseca, Eric M. Darling

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Characterizing Brain Cell Heterogeneity and the Role of Cell Subpopulations in Alzheimer’s Disease

Jessica S. Sadick

Processing Fixed and Stored Adipose-Derived Stem Cells for Quantitative Protein Array Assays

Jessica S. Sadick, Eric M. Darling

Accurately characterizing cellular subpopulations is essential for elucidating the mechanisms underlying normal and pathological biology. Isolation of specific cell types can be accomplished by labeling unique cell-associated proteins with fluorescent antibodies. Cell fixation is commonly used to prepare these samples and allow …

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Protein characterization of intracellular target-sorted, formalin-fixed cell subpopulations

Jessica S. Sadick, Molly E. Boutin, Diane Hoffman–Kim, Eric M. Darling

Cellular heterogeneity is inherent in most human tissues, making the investigation of specific cell types challenging. Here, we describe a novel, fixation/intracellular target-based sorting and protein extraction method to provide accurate protein characterization for cell subpopulations. Validation and feasibility tests were conducted …

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