2026
preprint
OpenAlex
Huihui Liu, Z Hong Zhou, Luowei Yuan, Bin Pang et autres
Finding high-fidelity CRISPR-Cas variants is critical for both the precision of in vitro DNA detection and the safety of in vivo gene-editing therapeutics. However, the large size of the Cas enzyme and the distinct selection criteria between its natural evolution and clinical …
cn, us
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Accès ouvert
2026
article
OpenAlex
Rongguang Lu, Kun Xi, Ruiyu Lin, Manchugondanahalli S. Krishna et autres
RNA secondary structures include double-stranded RNA (dsRNA) and single-stranded RNA (ssRNA) regions. dsRNAs can be targeted by chemically modified dsRNA-binding peptide nucleic acids (dbPNAs) via triplex formation, but inverted C-G pairs weaken affinity due to a relatively weakened Q⋅C-G triple. We show, …
cn, sg
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Accès ouvert
2026
article
OpenAlex
Qian Wu, Bo Wu, J. Liu, Lizhe Zhu et autres
ABSTRACT Aggregate science, which studies how molecular aggregates exhibit properties distinct from those of individual molecules, is fundamental to understanding molecular interactions in various fields, including chemistry, physics, and the life sciences. However, precisely controlling aggregate structures and their properties without altering …
cn
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Accès ouvert
2026
article
OpenAlex
Zhenyu Zhou, Lizhe Zhu
Nme1Cas9 is an encouraging genome-editing tool with high fidelity and compactness, but its applications are limited by poor catalytic efficiency compared with SpyCas9. Understanding the dynamic activation mechanism of the HNH nuclease domain is the key to breaking the kinetic bottleneck. Here, …
cn
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Accès ouvert
2025
article
OpenAlex
Fang Ye, Zhiyi Zhang, Binghao Zhang, Xinyu Li et autres
The hydroxycarboxylic acid receptors (HCAR2 and HCAR3), also known as prototypical metabolite-sensing receptors, are key targets for treating dyslipidemia and metabolic disorders. While HCAR2 activation, but not HCAR3 activation, is associated with side effects of cutaneous flushing, the structural features and ligand …
cn
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Accès ouvert
2025
article
OpenAlex
Chao Li, Agustin Enciso‐Martinez, Lizhe Zhu, Sarah A. Rotman et autres
Abstract Cancer‐associated fibroblasts (CAFs) are a major stromal cell type within the tumor microenvironment (TME), where they drive extracellular matrix remodeling and influence tumor progression through the secretion of bioactive molecules. Transforming growth factor‐β (TGF‐β) is a key regulator of CAF activation, …
nl
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2025
article
OpenAlex
Li Li, Kun Xi, Lizhe Zhu
Thermus thermophilus Argonaute ( Tt Ago) is a DNA-guided programmable endonuclease with emerging applications in genome engineering, yet the rate-determining dynamic mechanisms governing its transition from guide-target hybridization to catalytic activation remain unresolved. Here, we employ molecular dynamics simulations and the Traveling-salesman-based …
cn
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Accès ouvert
2025
preprint
OpenAlex
Leiye Yu, Haizhan Jiao, Bo Pang, Rujuan Ti et autres
Sphingosine-1-phosphate (S1P), a key metabolite of sphingolipids, plays crucial roles in a wide range of physiological and pathological processes. S1P primarily exerts its functions by binding to G protein-coupled sphingosine-1-phosphate receptors (S1PRs), which include five subtypes (S1PR1–5), thereby activating these receptors and …
cn
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Accès ouvert
2025
preprint
OpenAlex
Rongguang Lu, Kun Xi, Ruiyu Lin, Manchugondanahalli S. Krishna et autres
Abstract Peptide nucleic acid (PNA), a synthetic nucleic acid analog, exhibits substantial potential in biotechnology and therapeutic applications due to its high binding affinity and nuclease/protease resistance. Chemically modified PNAs capable of forming stable triplex structures with double-stranded RNAs (dsRNAs) under near-physiological …
hk, cn, sg
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2025
article
OpenAlex
Jianyu Yang, Huanlei Guo, Song Liu, Kun Xi et autres
Locating the transition states (TS) for the conformational changes of biomacromolecules is among the major tasks of biomolecular simulations, as they are the bottlenecks of motion encoding key mechanistic insights. However, identifying the short-lived TSs from (even abundant) simulation data has been …
cn, us
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Accès ouvert
2025
article
OpenAlex
Zhisen Yang, Hong Wei, Huihui Liu, Yang Cao et autres
Auxin is crucial in orchestrating diverse aspects of plant growth and development and modulating responses to environmental signals. The asymmetric spatiotemporal distribution of auxin generates local gradient patterns, which are regulated by both cellular auxin influx and efflux. The AUXIN1/LIKE-AUX1 (AUX1/LAX) family …
cn
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Accès ouvert
2025
article
OpenAlex
Wenzhuo Ma, Wenping Lyu, Lizhe Zhu
Clostridium butyricum Argonaute (CbAgo) can achieve DNA-guided DNA recognition and cleavage at physiological temperatures (~37 °C), making it a promising tool for gene editing. However, its significant off-target effects, particularly associated with the seed region (sites 2–8), pose challenges for precise gene …
cn
(code pays fourni par la source)