Yukihiro Harada

Hox/Meis-dependent gene-regulatory transition underlies cardiopharyngeal neural crest diversification

Akiyasu Iwase, Yasunobu Uchijima, Daiki Seya, Mayuko Kida et autres

Neural crest cells (NCCs) are multipotent migratory cells essential for cardiac development, yet the lineage trajectories and gene regulatory networks underlying their differentiation in the cardiopharyngeal region remain unclear. Here, we integrate single-cell RNA-seq, spatial transcriptomics, and multiomic analyses to construct a …

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Potential roles of β3-adrenergic receptor in heart rate regulation via sinus node activity and local adipocyte metabolism

Yukihiro Harada, Chih Chi Kao, Tomoe Ueyama, Alphonse Boché et autres

An Outflow Tract Myocardium‐Specific Enhancer at the Sema3c Locus During Heart Development

Yunce Wang, Yukihiro Harada, Tomoe Ueyama, Daiki Seya et autres

Sema3c is specifically expressed in the cardiac outflow tract (OFT) of the developing mouse heart and has been implicated in OFT polarization and great artery formation. However, the regulatory basis underlying its spatially restricted expression remains unclear. To investigate the mechanisms underlying …

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Author Reply to Peer Reviews of Hox-Meis-relayed spatial gene regulatory transition underlies cardiopharyngeal neural crest diversification revealed by multimodal analysis

Akiyasu Iwase, Yasunobu Uchijima, Daiki Seya, Mayuko Kida et autres

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Hox – Meis -relayed gene regulatory transition underlies cardiopharyngeal neural crest diversification

Akiyasu Iwase, Yasunobu Uchijima, Daiki Seya, Mayuko Kida et autres

Abstract Neural crest cells (NCCs) are multipotent migratory cells essential for cardiac development, yet the lineage trajectories and gene regulatory networks (GRNs) underlying their differentiation in the cardiopharyngeal region remain unclear. Here, we integrate single-cell RNA-seq, spatial transcriptomics, and multiomic analyses to …

jp, us (code pays fourni par la source)

Doxorubicin-induced sinus node dysfunction associated with mitochondria and nuclear impairment in a mouse model

Kazuki Kobayashi, Yukihiro Harada, Yusuke Suzuki, Nahoko Fukunishi et autres

Doxorubicin (DXB), an effective anti-cancer drug, is well documented for its cardiotoxicity in the ventricular myocardium. Although DXB-induced cardiomyopathy can cause arrhythmias, its impact on the cardiac conduction system remains unclear. We aimed to investigate whether DXB affects the function and subcellular …

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Computational and Experimental Analyses for Pathogenicity Prediction of ACVRL1 Missense Variants in Hereditary Hemorrhagic Telangiectasia

Toru Iwasa, Akihiro Urasaki, Yuki Kakihana, Nami Nagata‐Akaho et autres

Hereditary hemorrhagic telangiectasia (HHT) is a vascular disease caused by the defects of ALK1/ACVRL1 receptor signaling. In this study, we evaluated 25 recently identified ACVRL1 missense variants using multiple computational pathogenicity classifiers and experimentally characterized their signal transduction capacity. Three extracellular residue …

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c-Myc/microRNA-17-92 Axis Phase-Dependently Regulates PTEN and p21 Expression via ceRNA during Reprogramming to Mouse Pluripotent Stem Cells

Tomoaki Ishida, Tomoe Ueyama, Dai Ihara, Yukihiro Harada et autres

Induced pluripotent stem cells (iPSCs) are promising cell sources for regenerative medicine and disease modeling. iPSCs are commonly established by introducing the defined reprogramming factors Oct4, Sox2, Klf4, and c-Myc. However, iPSC reprogramming efficiency remains low. Although recent studies have identified microRNAs …

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Canonical Wnt signaling activation by chimeric antigen receptors for efficient cardiac differentiation from mouse embryonic stem cells

Takahiro Sogo, Shu Nakao, Tasuku Tsukamoto, Tomoe Ueyama et autres

BACKGROUND: Canonical Wnt signaling is involved in a variety of biological processes including stem cell renewal and differentiation, embryonic development, and tissue regeneration. Previous studies reported the stage-specific roles of the Wnt signaling in heart development. Canonical Wnt signal activation by recombinant …

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ETS‐dependent enhancers for endothelial‐specific expression of serum/glucocorticoid‐regulated kinase 1 during mouse embryo development

Yukihiro Harada, Toru Tanaka, Yuji Arai, Yoshie Isomoto et autres

Serum/glucocorticoid-regulated kinase 1 (SGK1) is predominantly expressed in endothelial cells of mouse embryos, and Sgk1 null mice show embryonic lethality due to impaired vascular formation. However, how the SGK1 expression is controlled in developing vasculature remains unknown. In this study, we first …

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Tmem100‐BAC‐EGFP mice to selectively mark and purify embryonic endothelial cells of large caliber arteries in mid‐gestational vascular formation

Yumi Kinugasa‐Katayama, Yusuke Watanabe, Takashi Hisamitsu, Yuichiro Arima et autres

Embryonic vascular development is achieved through the complex arrays of differentiation, proliferation, migration and mutual interaction of different cell types, and visualization as well as purification of unique cell populations are fundamental in studying its detailed mechanisms using in vivo experimental models. …

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Metabolic remodeling during somatic cell reprogramming to induced pluripotent stem cells: involvement of hypoxia-inducible factor 1

Tomoaki Ishida, Shu Nakao, Tomoe Ueyama, Yukihiro Harada et autres

Induced pluripotent stem cells (iPSCs) were first established from differentiated somatic cells by gene introduction of key transcription factors, OCT4, SOX2, KLF4, and c-MYC, over a decade ago. Although iPSCs can be applicable for regenerative medicine, disease modeling and drug screening, several …

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