Mario Pronk

Genome-Resolved Metagenomics Revealed the Functional Potential of Core Novel and Known Genera Key to Processes in Full-Scale Aerobic Granular Sludge Plants

Lucia Ruiz-Haddad, Dario Rangel Shaw, Muhammad Ali, Mario Pronk et autres

Microbial communities are critical for nutrient removal in aerobic granular sludge (AGS) wastewater treatment plants (WWTPs). Despite the stable long-term operation of full-scale AGS WWTPs, the microbial populations and functional traits sustaining stable long-term performance remain poorly resolved. To address this gap, …

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Experiences with aerobic granular sludge for large urban wastewater treatment plants

Andreas Giesen, Mark C.M. van Loosdrecht, Debby Berends, Asher Buchbut et autres

ABSTRACT Graphic shows on left hand side a three step Nereda SBR process cycle. Step 1 Feed and Draw. Step2 Aeration. Step 3 Fast Settling. On right hand a picture showing large SBRs of Ringend WWTP after retrofit to AGS and Hybrid-AGS …

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Erratum to “Extracellular polymeric substances in aerobic granular sludge under increasing salinity conditions” [Water Research (2026) 292/125313]

Le Min Chen, Sunanda Keisham, Hiroaki Tateno, Gijs Y. Kleine et autres

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PFAS in zeeschuim in Nederland

B Bokkers, Mario Pronk, J Herremans

Begin 2023 is een onderzoek gepubliceerd naar het voorkomen van PFAS in zeewater en zeeschuim aan de Vlaamse kust en de mogelijke impact daarvan op de PFAS-blootstelling voor mensen die bij recreatie aan de kust in contact kunnen komen met PFAS-houdend zeeschuim …

Extracellular polymeric substances in aerobic granular sludge under increasing salinity conditions

Le Min Chen, Sunanda Keisham, Hiroaki Tateno, Gijs Y. Kleine et autres

• “ Ca. Accumulibacter” is the dominant species from 1– 4% salinity. • The charge density of EPS and its charged glycoproteins increased with increasing salinity. • Increasing salinity induces the upregulation of glycan biosynthesis genes. • Putative glycoproteins produced by “ …

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Distinct roles of granules and flocs in aerobic granular sludge processes

Ling-Hang Li, Martin Pabst, Mark C.M. van Loosdrecht, Mario Pronk

Aerobic Granular Sludge (AGS) is an innovative and efficient biotechnology for wastewater treatment that has been successfully applied on full-scale worldwide. Full-scale municipal AGS systems typically contain both granular sludge (granules) and flocculent sludge (flocs). Studies on the different roles of granules …

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Identification of a surface protein in the extracellular polymeric substances of seawater-adapted aerobic granular sludge

Le Min Chen, Tessa Hofstra, Jelle J. H. Langedijk, Sebastian A. Andrei et autres

Identifying structural proteins within the extracellular polymeric substances (EPS) will provide a better understanding of the stability of aerobic granular sludge (AGS) and biofilms in general. In this work, an abundant surface protein was identified and localized in the extracellular matrix of …

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Genome-resolved metatranscriptomics unveils distinct microbial functionalities across aggregate sizes in aerobic granular sludge

A.Y.A. Mohamed, Laurence William Gill, Alejandro Javier Criado Monleon, Mario Pronk et autres

Microbial aggregates of different sizes in aerobic granular sludge (AGS) systems have been shown to exhibit distinct microbial community compositions. However, studies comparing the microbial activities of different-sized aggregates in AGS systems remain limited. In this study, genome-resolved metatranscriptomics was used to …

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Genome-Resolved Metatranscriptomics Provide Insights on Immigration Influence in Structuring Microbial Community Assembly of a Full-Scale Aerobic Granular Sludge Plant

A.Y.A. Mohamed, Laurence William Gill, Alejandro Javier Criado Monleon, Mario Pronk et autres

High Resolution Image Download MS PowerPoint Slide Understanding the relative influence of immigration and species sorting in wastewater treatment systems is essential, as bacteria in influent wastewater can significantly impact treatment system functionality. This study investigated the contribution of immigration to the …

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Characterization of the core microbial community across different aggregate sizes in full-scale aerobic granular sludge plants and their relevance to wastewater treatment performance

Lucia Ruiz-Haddad, Dario Rangel Shaw, Muhammad Ali, Mario Pronk et autres

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Microbiome structure and function in parallel full-scale aerobic granular sludge and activated sludge processes

Jennifer Ekholm, Frank Persson, Mark de Blois, Oskar Modin et autres

Aerobic granular sludge (AGS) and conventional activated sludge (CAS) are two different biological wastewater treatment processes. AGS consists of self-immobilised microorganisms that are transformed into spherical biofilms, whereas CAS has floccular sludge of lower density. In this study, we investigated the treatment …

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Effects of salinity on glycerol conversion and biological phosphorus removal by aerobic granular sludge

Ali Elahinik, Fleur de Clercq, Martin Pabst, Dimitrios Xevgenos et autres

Industrial wastewater often has high levels of salt, either due to seawater or e.g. sodium chloride (NaCl) usage in the processing. Previous work indicated that aerobic granular sludge (AGS) is differently affected by seawater or saline water at similar osmotic strength. Here …

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