Manuela Rigno

Passenger lymphocyte syndrome after living-donor kidney transplantation with minor ABO incompatibility: a case report.

Alessandra Sandini, Teresa Giulia Da Molin, Gabriella Errigo, Barbara Sartori et autres

it (code pays fourni par la source)

Iptacopan/LNP023 and rituximab as rescue therapy in a patient with systemic lupus erythematosus-associated atypical haemolytic uraemic syndrome

Francesco Benvenuti, Marta Proglio, Manuela Rigno, David Sorbo

Systemic lupus erythematosus (SLE) is rarely associated with atypical haemolytic uraemic syndrome, leading to a poor prognosis with multi-system involvement. The pathogenesis seems to be driven by hyperactivation of alternate pathways of complement cascade and patients should be treated with prompt administration …

it (code pays fourni par la source)

Palliative care is related to less aggressive end-of-life treatment in haematology-oncology: a retrospective cohort study

Davide Facchinelli, Corinna Greco, Manuela Rigno, Daniela Menon et autres

OBJECTIVES: Patients with haematological malignancies (HM) experience high-intensity medical care near the end of life (EOL), have low rates of hospice and palliative care (PC) use and are more likely to die in the hospital. We compared the quality indicators for EOL …

it (code pays fourni par la source)

P1691: PALLIATIVE CARE IN HEMATOLOGICAL PATIENTS IS RELATED TO LESS AGGRESSIVE TREATMENT IN THE END-OF-LIFE, A RETROSPECTIVE STUDY.

Davide Facchinelli, Corinna Greco, Manuela Rigno, Daniela Menon et autres

Topic: 35. Quality of life and palliative care Background: Patients with blood cancers experience high-intensity medical care near the end of life (EOL), have low rates of hospice and palliative care (PC), and are more likely to die in a hospital. Offering …

it (code pays fourni par la source)

Generation of mesenchymal stromal cells from cord blood: evaluation of in vitro quality parameters prior to clinical use

Eliana Amati, Sabrina Sella, Omar Perbellini, Alberta Alghisi et autres

BACKGROUND: Increasing evidence suggests the safety and efficacy of mesenchymal stromal cells (MSC) as advanced therapy medicinal products because of their immunomodulatory properties and supportive role in hematopoiesis. Although bone marrow remains the most common source for obtaining off-the-shelf MSC, cord blood …

it (code pays fourni par la source)

Additional file 6: Table S3. of Generation of mesenchymal stromal cells from cord blood: evaluation of in vitro quality parameters prior to clinical use

Eliana Amati, Sabrina Sella, Omar Perbellini, Alberta Alghisi et autres

Five-color mAb combinations used for immunophenotypic analysis of inflammatory MSC priming. (DOCX 14 kb)

Additional file 10: Figure S7. of Generation of mesenchymal stromal cells from cord blood: evaluation of in vitro quality parameters prior to clinical use

Eliana Amati, Sabrina Sella, Omar Perbellini, Alberta Alghisi et autres

SL-CBMSC immunophenotypic analysis. Characterization of SL-MSC (nâ =â 6) by flow cytometry using a panel of 14 cell surface markers. Boxes extend from 25th percentile to the 75th percentile, the middle line represents median value and the whiskers extend from minimum to …

Additional file 7: Figure S4. of Generation of mesenchymal stromal cells from cord blood: evaluation of in vitro quality parameters prior to clinical use

Eliana Amati, Sabrina Sella, Omar Perbellini, Alberta Alghisi et autres

Immunomodulation assay. Flow cytometry analysis of thawed PBMC before (A) and after (B) overnight resting. The percentage of monocytes of one representative sample is shown. (C) Proliferation ratio between the percentage of CD45+ proliferation at primed and resting conditions, at the different …

Additional file 4: Figure S3. of Generation of mesenchymal stromal cells from cord blood: evaluation of in vitro quality parameters prior to clinical use

Eliana Amati, Sabrina Sella, Omar Perbellini, Alberta Alghisi et autres

Stability of endogenous reference genes (TBP and YWHAZ) under differentiation conditions. Quantitative RT-PCR analysis of TBP normalized with respect to YWHAZ (A-C) and vice versa (D-F) under osteogenic (A and D), chondrogenic (B and E) and adipogenic (C and F) differentiation conditions. …

Additional file 3: Table S1. of Generation of mesenchymal stromal cells from cord blood: evaluation of in vitro quality parameters prior to clinical use

Eliana Amati, Sabrina Sella, Omar Perbellini, Alberta Alghisi et autres

Genes and primer sequences used for quantitative real-time PCR (RT-PCR). (DOCX 13 kb)

Additional file 6: Table S3. of Generation of mesenchymal stromal cells from cord blood: evaluation of in vitro quality parameters prior to clinical use

Eliana Amati, Sabrina Sella, Omar Perbellini, Alberta Alghisi et autres

Five-color mAb combinations used for immunophenotypic analysis of inflammatory MSC priming. (DOCX 14 kb)

Additional file 2: Figure S2. of Generation of mesenchymal stromal cells from cord blood: evaluation of in vitro quality parameters prior to clinical use

Eliana Amati, Sabrina Sella, Omar Perbellini, Alberta Alghisi et autres

Dexamethasone scheduling in MNC culture. The supplement was added in standard medium until the detection of MSC colonies (nâ =â 16 CB units) or alternatively added for the first week of MNC culture only (nâ =â 34). (DOCX 120 kb)