Xinyue Ma

Genome-wide identification and expression pattern analysis of the Medicago sativa 4-coumarate: CoA ligase (4CL) gene family

Haiyue Lei, Liu S, Y ZHANG, Xinyue Ma et autres

4-Coumarate: CoA ligase (4CL) is a pivotal enzyme in the phenylpropanoid metabolic pathway and plays a crucial role in plant growth, development, and stress responses. In this study, a genome-wide analysis was conducted to identify a total of 43 Ms4CL genes ( …

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Conditionally Significant eQTL and TWAS Analyses Identify MsRD26 as a Major Candidate Positive Regulator of Salt Tolerance in Medicago sativa L

Lin Chen, Yuqi Zhang, Xinyue Ma, Jinpeng Bi et autres

Natural variation in gene expression bridges genetic polymorphisms and phenotypic divergence, yet the regulatory architecture underlying salt stress responses remains largely unexplored in alfalfa. Here, we generated 528 RNA-seq libraries from 176 alfalfa accessions under well-watered and salt stress conditions and identified …

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Genome-wide identification and abiotic stress expression analysis of laccase gene family in alfalfa

Li Zhao, Haiyue Lei, Zhengqin Xiao, Y ZHANG et autres

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Characterization of the BPC genes in alfalfa and functional verification of MsBPC10 in salt tolerance

Li Zhao, Xianyang Li, Xinyue Ma, Yuqi Zhang et autres

BACKGROUND: Soil salinization severely restricts the growth and forage yield of Medicago sativa (alfalfa), a globally critical leguminous forage crop, yet the regulatory mechanisms governing its salt tolerance remain incompletely understood-especially the role of the BASIC PENTACYSTEINE (BPC) transcription factor family, which …

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Single-nucleus RNA-seq and ATAC-seq analyses provide molecular insights into cadmium-stress response in alfalfa roots

Yuqi Zhang, Hao Liu, Ming Xu, Mengjia Xie et autres

Abstract Soil cadmium (Cd) pollution threatens global food security. Elucidating plant cellular responses to Cd stress is critical for the breeding of low-Cd-accumulating crops. Here, we investigated Cd-responsive regulatory mechanisms in alfalfa at single-cell resolution and identified key Cd-associated genes. Eight major …

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Genome-wide identification and analysis of the abiotic stress responsiveness of the heat shock protein 90 gene family in Medicago sativa L

Hao Liu, Yuqi Zhang, Xianyang Li, Li Zhao et autres

BACKGROUND: Heat shock protein 90 (HSP90) is a highly conserved class of cellular chaperone proteins that are widely present in plants and are involved in the response to various biotic and abiotic stresses. The HSP90 gene has been extensively studied across numerous …

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Genome-wide characterization of NHX gene family in Medicago sativa under abiotic stress

Yuqi Zhang, Xinyue Ma, Hao Liu, Li Zhao et autres

Soil salinization is one of the major abiotic stresses limiting global crop production. As key regulators of plant salt tolerance, the NHX gene family remains systematically uncharacterized in alfalfa (Medicago sativa). In this study, genome-wide analysis identified 35 NHX family genes (MsNHXs) …

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Genome-wide identification and expression pattern analysis of the CPP transcription factor family in Medicago sativa L

Xinyue Ma, Xianyang Li, Li Zhao, Yuqi Zhang et autres

BACKGROUND: The Cysteine-rich Polycomb-like Protein (CPP) gene family is a small transcription factor family commonly found in animals and plants. It plays a crucial regulatory role in their reproductive development. To date, this gene family has been characterized in various species, such …

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Progress and Prospects in FRET for the Investigation of Protein–Protein Interactions

Yue Zhang, Xinyue Ma, Meihua Zhu, Vivien Ya‐Fan Wang et autres

Protein-protein interactions (PPIs) play a crucial role in various biological processes, including signal transduction, transcriptional regulation, and metabolic pathways. Over the years, many methods have been developed to study PPIs, such as yeast two-hybrid (Y2H), co-immunoprecipitation (Co-IP), pull-down assays, and surface plasmon …

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The DEC2‐SCN2A Axis is Essential for the Anticonvulsant Effects of Cannabidiol by Modulating Neuronal Plasticity

Huifang Song, Yifan Wang, Lili Wang, Chang Guo et autres

Impairment of neuronal plasticity is involved in a spectrum of neurological disorders such as epilepsy, yet its regulatory mechanisms remain incompletely understood. Here, it is reported that the basic helix-loop-helix transcription factor DEC2 serves as a pivotal regulator of both neuronal plasticity …

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Optimization of fermentation medium and fermentation conditions of biocontrol strain A192 against pepper anthracnose

Xinyue Ma, Mingyang Ma, Xiaoxu Sun, Lingyue Zhu et autres

Alkaline Phosphatase and ATG4B Sequentially Activated Fluorescent Probe for Cancer Cell-Specific Live Imaging of Autophagy

Yaxin Zheng, Xinyue Ma, Shuyao Zhou, Wenwen Lei et autres

Tracking autophagy in cancer cells is crucial for enhancing cancer therapies. Existing methods are often inefficient and cannot distinguish cancer from normal cells during autophagy. Herein, a sequentially activated peptide probe, NBD-1p-Dabcyl, was developed for achieving cancer cell-specific imaging of autophagy. The …

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