Accès ouvert
2020
article
OpenAlex
Yanli Zheng, Jie Li, Baiyang Wang, Jiamei Han et autres
Establishment of production platforms through prokaryotic engineering in microbial organisms would be one of the most efficient means for chemicals, protein, and biofuels production. Despite the fact that CRISPR (clustered regularly interspaced short palindromic repeats)-based technologies have readily emerged as powerful and …
cn
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Accès ouvert
2019
article
OpenAlex
Yanli Zheng, Jiamei Han, Baiyang Wang, Runxia Li et autres
Application of CRISPR-based technologies in non-model microorganisms is currently very limited. Here, we reported efficient genome engineering of an important industrial microorganism, Zymomonas mobilis, by repurposing the endogenous Type I-F CRISPR-Cas system upon its functional characterization. This toolkit included a series of …
cn
(code pays fourni par la source)
Accès ouvert
2019
preprint
OpenAlex
Yanli Zheng, Jiamei Han, Wenyao Liang, Runxia Li et autres
ABSTRACT Establishment of production platform organisms through prokaryotic engineering represents an efficient means to generate alternatives for yielding renewable biochemicals and biofuels from sustainable resources. Zymomonas mobilis , a natural facultative anaerobic ethanologen, possesses many attractive physiological attributes, making it an important …
cn
(code pays fourni par la source)