Panagiotis G. Adamopoulos

Engineering the Future of Precision Medicine: A Comprehensive Guide to RNA Therapeutics

Konstantina Athanasopoulou, Glykeria N. Daneva, Vasiliki-Ioanna Michalopoulou, Maria R. Stamelou et autres

RNA therapeutics have evolved from passive genetic intermediaries into highly programmable platforms, fundamentally transforming the landscape of precision medicine. This comprehensive review examines the molecular architecture and mechanisms of established platforms in the clinical setting, including mRNA, antisense oligonucleotides (ASOs), small interfering …

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Wastewater genomic surveillance of SARS-CoV-2 by nanopore sequencing uncovers JN.1 prevalence and novel spike mutational hotspots in Athens during winter 2024–2025

Marios A. Diamantopoulos, Panagiotis G. Adamopoulos, Konstantina Athanasopoulou, Anastasia Zafeiriadou et autres

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Identification of Novel Alternative Transcripts of the Human ALKBH Gene Family and Investigation of Their Unique Expression Signatures in Cancer Cells

Konstantina Athanasopoulou, Vasiliki-Ioanna Michalopoulou, Panagiotis Tsiakanikas, Andreas Scorilas et autres

The human ALKBH gene family comprises nine Fe2+/α-ketoglutarate-dependent dioxygenases that catalyze the oxidative demethylation of DNA, RNA, and proteins, thereby influencing key cellular processes. Consequently, dysregulation of these enzymes has been implicated in various human diseases, particularly cancer. Although the transcriptomic profiles …

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A versatile type VI CRISPR-based approach for targeted m 6 A demethylation in mRNAs

Panagiotis G. Adamopoulos, Konstantina Athanasopoulou, Andreas Scorilas

Epitranscriptomics, a rapidly evolving field mainly driven by massive parallel sequencing technologies, explores post-transcriptional RNA modifications. N 6 -methyladenosine (m 6 A) has emerged as the most prominent and dynamically regulated modification in human mRNAs, being implicated in the regulation of diverse …

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Deciphering the m6A Epitranscriptomic Landscape of mRNAs in Breast Cancer Cells

Konstantina Athanasopoulou, Panagiotis G. Adamopoulos, Panagiotis Tsiakanikas, Glykeria N. Daneva et autres

N6-methyladenosine (m6A), the most prevalent modification in mRNAs, influences mRNA stability, splicing, and translation. Dysregulation of m6A patterns has been linked to various diseases, including cancer, highlighting its significance in cellular homeostasis. However, accurate detection and precise quantification of m6A sites within …

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Decoding the Transcriptional Complexity of the Human BRCA2 DNA Repair Gene Using Hybrid-seq

Panagiotis G. Adamopoulos, Michaela A. Boti, Konstantina Athanasopoulou, Panagiotis Tsiakanikas et autres

BRCA2 plays a pivotal role in DNA repair and tumor suppression, with its dysregulation linked to breast and gynecological cancers. Despite the importance of BRCA2, its transcriptional complexity remains poorly understood due to the gene's size and intricate alternative splicing patterns. This …

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Exploring the m5C epitranscriptome of mRNAs in breast cancer cells through genome engineering and long-read sequencing approaches

Konstantina Athanasopoulou, Panagiotis G. Adamopoulos, Panagiotis Tsiakanikas, Andreas Scorilas

Abstract Epitranscriptomics has emerged as a rapidly evolving field that focused on studying post-transcriptional RNA modifications and their role in spatiotemporal regulation of gene expression. N6-methyladenosine (m 6 A) and 5-methylcytosine (m 5 C) represent the most extensively studied modifications on mRNAs. …

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Integrating Artificial Intelligence in Next-Generation Sequencing: Advances, Challenges, and Future Directions

Konstantina Athanasopoulou, Vasiliki-Ioanna Michalopoulou, Andreas Scorilas, Panagiotis G. Adamopoulos

The integration of artificial intelligence (AI) into next-generation sequencing (NGS) has revolutionized genomics, offering unprecedented advancements in data analysis, accuracy, and scalability. This review explores the synergistic relationship between AI and NGS, highlighting its transformative impact across genomic research and clinical applications. …

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Characterization of novel ACE2 mRNA transcripts: The potential role of alternative splicing in SARS-CoV-2 infection

Panagiotis G. Adamopoulos, Natalia Bartzoka, Panagiotis Tsiakanikas, Andreas Scorilas

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Small non-coding RNAs as diagnostic, prognostic and predictive biomarkers of gynecological cancers: an update

Marios A. Diamantopoulos, Panagiotis G. Adamopoulos, Andreas Scorilas

INTRODUCTION: Non-coding RNAs (ncRNAs) comprise a heterogeneous cluster of RNA molecules. Emerging evidence suggests their involvement in various aspects of tumorigenesis, particularly in gynecological malignancies. Notably, ncRNAs have been implicated as mediators within tumor signaling pathways, exerting their influence through interactions with …

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Kallikrein-related peptidases: mechanistic understanding for potential therapeutic targeting in cancer

Glykeria N. Daneva, Panagiotis Tsiakanikas, Panagiotis G. Adamopoulos, Andreas Scorilas

INTRODUCTION: Human kallikrein-related peptidases (KLKs) represent a subgroup of 15 serine endopeptidases involved in various physiological processes and pathologies, including cancer. AREAS COVERED: This review aims to provide a comprehensive overview of the KLK family, highlighting their genomic structure, expression profiles and …

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New insights into the dynamics of m6A epitranscriptome: hybrid-seq identifies novel mRNAs of the m6A writers METTL3/14

Konstantina Athanasopoulou, Panagiotis G. Adamopoulos, Andreas Scorilas

Background: N6-methyladenosine (m6A), a prevalent mRNA modification, is dynamically regulated by methyltransferases, including METTL3 and METTL14.Materials & methods: In the current study, we employed a custom hybrid-seq method to identify novel METTL3/14 transcripts, explore their protein-coding capacities and predict the putative role …

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