Accès ouvert
2026
article
OpenAlex
Alison R. Sherwood, Sophie Paradis, Gernot G. Presting
We have reported the first mitochondrial (GenBank accession PV035080) and chloroplast (GenBank accession PV035081) genomes for a representative of the gigartinalean family Dumontiaceae (Gibsmithia punonomaewa). High-throughput sequencing yielded both organellar genomes for the holotype specimen of Gibsmithia punonomaewa, a recently described species …
us
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Accès ouvert
2022
preprint
OpenAlex
Daniel Joseph Laspisa, Kevin L. Schneider, Gernot G. Presting
Abstract Genome assemblies based on long read sequencing technology have revolutionized the assembly of repeat-rich centromere regions. However, because maize centromeres are highly enriched for the tandem repeat CentC and centromeric retrotransposons (CR), automated genome assembly left gaps even in the excellent …
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Accès ouvert
2020
article
OpenAlex
Jianing Liu, Arun Somwarpet Seetharam, Kapeel M. Chougule, Shujun Ou et autres
Creating gapless telomere-to-telomere assemblies of complex genomes is one of the ultimate challenges in genomics. We use two independent assemblies and an optical map-based merging pipeline to produce a maize genome (B73-Ab10) composed of 63 contigs and a contig N50 of 162 …
us
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Accès ouvert
2020
preprint
OpenAlex
Jianing Liu, Arun Somwarpet Seetharam, Kapeel M. Chougule, Shujun Ou et autres
Creating gapless telomere-to-telomere assemblies of complex genomes is one of the ultimate challenges in genomics. We used long read technologies and an optical map based approach to produce a maize genome assembly composed of only 63 contigs. The B73-Ab10 genome includes gapless …
us
(code pays fourni par la source)
Accès ouvert
2018
review
OpenAlex
Gernot G. Presting
us
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Accès ouvert
2017
article
OpenAlex
Yinping Jiao, Paul S. Peluso, Jinghua Shi, Tiffany Y Liang et autres
An improved reference genome for maize, using single-molecule sequencing and high-resolution optical mapping, enables characterization of structural variation and repetitive regions, and identifies lineage expansions of transposable elements that are unique to maize. The maize genome was initially reported in 2009 but …
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Accès ouvert
2016
preprint
OpenAlex
Yinping Jiao, Paul S. Peluso, Jinghua Shi, Tiffany Y Liang et autres
ABSTRACT Complete and accurate reference genomes and annotations provide fundamental tools for characterization of genetic and functional variation. These resources facilitate elucidation of biological processes and support translation of research findings into improved and sustainable agricultural technologies. Many reference genomes for crop …
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Accès ouvert
2016
article
OpenAlex
Thomas Wolfgruber, Megan Mariko Nakashima, Kevin L. Schneider, Anupma Sharma et autres
The ancestral centromeres of maize contain long stretches of the tandemly arranged CentC repeat. The abundance of tandem DNA repeats and centromeric retrotransposons (CR) has presented a significant challenge to completely assembling centromeres using traditional sequencing methods. Here, we report a nearly …
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Accès ouvert
2016
article
OpenAlex
Kevin L. Schneider, Zidian Xie, Thomas Wolfgruber, Gernot G. Presting
Functional centromeres, the chromosomal sites of spindle attachment during cell division, are marked epigenetically by the centromere-specific histone H3 variant cenH3 and typically contain long stretches of centromere-specific tandem DNA repeats (∼1.8 Mb in maize). In 23 inbreds of domesticated maize chosen …
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2016
book-chapter
OpenAlex
Zidian Xie, Gernot G. Presting
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Accès ouvert
2014
article
OpenAlex
Alison R. Sherwood, Amy Lynn Carlile, Jessica M Neumann, John Patrick Kociolek et autres
BACKGROUND: A remarkable range of environmental conditions is present in the Hawaiian Islands due to their gradients of elevation, rainfall and island age. Despite being well known as a location for the study of evolutionary processes and island biogeography, little is known …
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Accès ouvert
2014
article
OpenAlex
Anupma Sharma, Gernot G. Presting
Centromeric retrotransposons (CRs) constitute a family of plant retroelements, some of which have the ability to target their insertion almost exclusively to the functional centromeres. Our exhaustive analysis of CR family members in four grass genomes revealed not only horizontal transfer (HT) …
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