Sophie Biver

Identification and Characterization of a Halotolerant, Cold-Active Marine Endo-β-1,4-Glucanase by Using Functional Metagenomics of Seaweed-Associated Microbiota

Marjolaine Martin, Sophie Biver, Sébastien Steels, Tristan Barbeyron et autres

A metagenomic library was constructed from microorganisms associated with the brown alga Ascophyllum nodosum. Functional screening of this library revealed 13 novel putative esterase loci and two glycoside hydrolase loci. Sequence and gene cluster analysis showed the wide diversity of the identified …

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Two promising alkaline β-glucosidases isolated by functional metagenomics from agricultural soil, including one showing high tolerance towards harsh detergents, oxidants and glucose

Sophie Biver, Aurore Stroobants, Daniel Portetelle, Micheline Vandenbol

New β-glucosidase activities were identified by screening metagenomic libraries constructed with DNA isolated from the topsoil of a winter wheat field. Two of the corresponding proteins, displaying an unusual preference for alkaline conditions, were selected for purification by Ni-NTA chromatography. AS-Esc6, a …

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Characterization of a new oxidant-stable serine protease isolated by functional metagenomics

Sophie Biver, Daniel Portetelle, Micheline Vandenbol

A novel serine protease gene, SBcas3.3, was identified by functional screening of a forest-soil metagenomic library on agar plates supplemented with AZCL-casein. Overproduction in Escherichia coli revealed that the enzyme is produced as a 770-amino-acid precursor which is processed to a mature …

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Characterization of new bacterial glycoside hydrolases isolated from agricultural soils using a functional metagenomic approach

Sophie Biver, B. Dubois, Aurore Stroobants, Daniel Portetelle et autres

Microorganisms play key roles in soil ecosystem functioning, notably through their ability to degrade plant cell wall polymers. For this, bacteria and fungi produce various enzymes such as cellulases, xylanases, glucosidases, esterases or laccases. Finding new enzymes hydrolyzing cellulose, hemicellulose or lignin …

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Bacillus subtilis as a Tool for Screening Soil Metagenomic Libraries for Antimicrobial Activities

Sophie Biver

Finding new antimicrobial activities by functional metagenomics has been shown to depend on the heterologous host used to express the foreign DNA. Therefore, efforts are devoted to developing new tools for constructing metagenomic libraries in shuttle vectors replicatable in phylogenetically distinct hosts. …

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Biomass hydrolyzing enzymes identified by functional screening of a metagenomic library from algal biofilms.

Marjolaine Martin, Sophie Biver, Tristan Barbeyron, Gurvan Michel et autres

Biomass hydrolyzing enzymes are increasingly searched for the production of biofuels and renewable chemical compounds using biomass. Microorganisms living on algaes are an interesting reservoir of biomass hydrolyzing enzymes, as they are in constant interaction with algal biomass. Therefore, we are interested …

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Microorganisms living on algae : An interesting reservoir of enzymes hydrolyzing algal biomass

Marjolaine Martin, Sophie Biver, Tristan Barbeyron, Gurvan Michel et autres

Algal polysaccharides are increasingly used in food industry for their gelling properties and in pharmacology for their therapeutic properties. Furthermore, increasingly interest is taken on algae for their use in the production of biofuels and bioenergies. To purify algal polysaccharides and degrade …

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Characterization of three new carboxylic ester hydrolases isolated by functional screening of a forest soil metagenomic library

Sophie Biver, Micheline Vandenbol

Three new lipolytic genes were isolated from a forest soil metagenomic library by functional screening on tributyrin agar plates. The genes SBLip1, SBLip2 and SBLip5.1 respectively encode polypeptides of 445, 346 and 316 amino acids. Phylogenetic analyses revealed that SBLip2 and SBLip5.1 …

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Identification of new microbial enzymes from forest and marine ecosystems by functional metagenomics

Marjolaine Martin, Sophie Biver, Tristan Barbeyron, Gurvan Michel et autres

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Isolation of two antimicrobial activities using a functional metagenomic approach

Sophie Biver, Christel Mattéotti, Micheline Vandenbol

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Screening for new antimicrobial activities in a forest soil by functional metagenomics

Sophie Biver, Daniel Portetelle, Micheline Vandenbol

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Multicopy suppression screen in a Saccharomyces cerevisiae strain lacking the Rab GTPase-activating protein Msb3p

Sophie Biver, Daniel Portetelle, Micheline Vandenbol

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