Cindy Given

ESBL plasmid compatibility with the surrounding microbial community influences ESBL gene survival under CRISPR-antimicrobial targeting

Cindy Given, Ilmur Jonsdottir, Krista Norvasuo, Petra Paananen et autres

Abstract Bacteria often acquire resistance against antibiotics through the transfer of conjugative resistance plasmids. Hence, it is vital to develop strategies to mitigate the dispersal of antimicrobial resistance (AMR). CRISPR-based antimicrobial tools offer a sequence-specific solution to diminish and restrict the dissemination …

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Preceding Host History of Conjugative Resistance Plasmids Affects Intra- and Interspecific Transfer Potential from Biofilm

Ilmur Jonsdottir, Cindy Given, Reetta K. Penttinen, Matti Jalasvuori

Antimicrobial resistance (AMR) is a major global public health threat which can rapidly spread in microbial communities via conjugative plasmids. Here, we advance with evolutionary rescue via conjugation in a more natural setting, namely, biofilm, and incorporate a broad-host range plasmid RP4 …

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Plasmid Viability Depends on the Ecological Setting of Hosts within a Multiplasmid Community

Cindy Given, Reetta K. Penttinen, Matti Jalasvuori

Conjugative and mobilizable plasmids are ubiquitous in bacterial systems. Several different plasmids can compete within a single bacterial community. We here show that the ecological setting of the host bacteria has a notable effect on the survival of individual plasmids. Selection for …

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Multi-plasmid clash in a bacterial community: plasmid viability depends on the ecological setting of hosts

Cindy Given, Reetta K. Penttinen, Matti Jalasvuori

Abstract Plasmids are genetic elements that disperse horizontally between different strains and species of bacteria and a major factor in the dissemination of virulence factors and antibiotic resistance. Understanding the ecology of plasmids has a notable anthropocentric value and therefore the interactions …

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Indirect Selection against Antibiotic Resistance via Specialized Plasmid-Dependent Bacteriophages

Reetta K. Penttinen, Cindy Given, Matti Jalasvuori

Antibiotic resistance genes of important Gram-negative bacterial pathogens are residing in mobile genetic elements such as conjugative plasmids. These elements rapidly disperse between cells when antibiotics are present and hence our continuous use of antimicrobials selects for elements that often harbor multiple …

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Beta-Lactam Sensitive Bacteria Can Acquire ESBL-Resistance via Conjugation after Long-Term Exposure to Lethal Antibiotic Concentration

Pilvi Ruotsalainen, Cindy Given, Reetta K. Penttinen, Matti Jalasvuori

Beta-lactams are commonly used antibiotics that prevent cell-wall biosynthesis. Beta-lactam sensitive bacteria can acquire conjugative resistance elements and hence become resistant even after being exposed to lethal (above minimum inhibitory) antibiotic concentrations. Here we show that neither the length of antibiotic exposure …

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Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna

Cindy Given, Elina Häikiö, Manoj Kumar, RIITTA M. NISSINEN

The rapid development in the next-generation sequencing methods in the recent years have provided more information regarding the community structures and functions of endophytic bacteria. However, the assembly rules of these communities in different plant tissues are currently poorly understood. The aim …

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Assembly and functioning of endophytic bacterial communities in arcto-alpine pioneer plant Oxyria digyna

Cindy Given

Plant microbiomes consist of diverse communities of microorganisms, among which bacteria are highly abundant. The microbiomes are crucial for plants as they rely on their microbial associates for many essential functions. The goal of this thesis was to study the functional diversity …