Christopher Boddington

Quantitative analysis of competitive cytokine signaling predicts tissue thresholds for the propagation of macrophage activation

James Bagnall, Christopher Boddington, Hazel England, Ruth Brignall et autres

transcriptional response correlated with the extent of NF-κB signaling in the cells and their size. We compared the rates of TNF-α production and uptake in macrophages and mouse embryonic fibroblasts and generated a mathematical model to explore the heterogeneity in the response …

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Integration of Kinase and Calcium Signaling at the Level of Chromatin Underlies Inducible Gene Activation in T Cells

Ruth Brignall, Pierre Cauchy, Sarah L. Bevington, Bethany Gorman et autres

TCR signaling pathways cooperate to activate the inducible transcription factors NF-κB, NFAT, and AP-1. In this study, using the calcium ionophore ionomycin and/or PMA on Jurkat T cells, we show that the gene expression program associated with activation of TCR signaling is …

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Signal transduction controls heterogeneous NF-κB dynamics and target gene expression through cytokine-specific refractory states

Antony D. Adamson, Christopher Boddington, Polly Downton, William L. Rowe et autres

Cells respond dynamically to pulsatile cytokine stimulation. Here we report that single, or well-spaced pulses of TNFα (>100 min apart) give a high probability of NF-κB activation. However, fewer cells respond to shorter pulse intervals (<100 min) suggesting a heterogeneous refractory state. …

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Quantitative dynamic imaging of immune cell signalling using lentiviral gene transfer

James Bagnall, Christopher Boddington, James Boyd, Ruth Brignall et autres

Live-cell imaging of fluorescent fusion proteins has transformed our understanding of mammalian cell signalling and function. However, some cellular systems such as immune cells are unsuitable or refractory to many existing transgene delivery methods thus limiting systematic analyses. Here, a flexible lentiviral …

gb (code pays fourni par la source)

An interdisciplinary analysis of inflammatory signalling dynamics in single cells

Christopher Boddington

An interdisciplinary analysis of inflammatory signalling dynamics in single cells Christopher Boddington (2015) A thesis submitted to the University of Manchester for the degree of Doctor of Philosophy Immune cells must accurately interpret environmental signals to make robust cell fate decisions and …