Quan Zhou

Epitope‐Resolved Digital SERS Profiling of Structurally Dynamic Antigens via a Multi‐Epitope Bispecific Antibody Framework

Jing Wang, Quan Zhou, Kym Lowry, Christopher B. Howard et autres

ABSTRACT Digital surface‐enhanced Raman scattering (SERS) immunoassays digitize epitope binding events to achieve ultrasensitive protein detection. However, existing implementations predominantly rely on single‐epitope recognition, yielding 1D molecular view of antigen structure and obscuring how conformational heterogeneity or mutation‐induced changes affect epitopes. This …

cn, au (code pays fourni par la source)

Multifactor authentication in extracellular vesicle analysis: methods and approaches to address the heterogeneity problem

Quan Zhou, Richard J. Lobb, Alain Wuethrich, Matt Trau

au (code pays fourni par la source)

Early-stage multi-cancer detection through a plasma extracellular vesicle protein signature

Richard J. Lobb, Quan Zhou, David Fielding, Kekoolani S. Visan et autres

Small extracellular vesicles (sEVs) offer a promising, non-invasive method for cancer detection. Despite global research efforts, successful translation of sEV-based diagnostics remains limited. In this study, we identify a 4-protein sEV biomarker panel (thrombospondin-1, nidogen-1, pentraxin-3, and versican) based on proteomic profiles …

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Monitoring glioblastoma extracellular vesicle evolution using a nanodiagnostic platform to detect glioma stem cells driving recurrent disease

Zhen Zhang, Richard J. Lobb, Paul A. Tooney, Jing Wang et autres

Assessing therapeutic response in glioblastoma (GBM) is a major factor limiting the clinical development of effective therapies. The intracranial location limits serial biopsies and only provides an intermittent view of the tumor molecular profile from the initial resection. Liquid biopsy techniques, specifically …

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A digital SERS platform powered by bispecific antibody fragments for SARS-CoV-2 diagnostics

Jing Wang, Quan Zhou, Kym Lowry, Christopher B. Howard et autres

Surface-enhanced Raman scattering (SERS) immunoassays are powerful analytical tools for protein detection while typically rely on the availability of suitable antibodies. Compared to full-length antibodies, antibody fragments provide advantages such as rapid and cost-effective production. However, oriented conjugation of antibody fragments to …

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Physicochemical Tuning of Bispecific Antibody Fragment Immobilization for Robust Digital SERS Biosensing

Jing Wang, Quan Zhou, Christopher B. Howard, Matt Trau

Surface-enhanced Raman scattering (SERS) biosensing platforms offer exceptional sensitivity and multiplexing capabilities for biomarker detection, but their performance is often limited by uncontrolled ligand orientation on nanotags. To address this, we developed a SERS nanotag incorporating a bispecific antibody fragment (BsAb) engineered …

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Multi-factor authentication of extracellular vesicles in clinical settings

Quan Zhou

Multiplex Cardiac Biomarker Profiling for Precision Coronary Artery Disease Risk Assessment Using SERS with Cubic Core–Satellite Nanostructures

Tuotuo Zhang, Bin Li, Alain Wuethrich, Huixing Liu et autres

An early and accurate risk assessment of coronary artery disease (CAD) is crucial for implementing effective health management strategies before the disease progresses to severe or life-threatening conditions such as myocardial infarction (MI). To this end, we present a surface-enhanced Raman scattering …

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Development and application of multivalent nanobody-functionalized plasmonic probes in SERS sensing platforms

Jing Wang, Quan Zhou, Kym Lowry, Christopher B. Howard et autres

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Electromagnetic Manipulation of Single Particles for Studying Cell Adhesion Dynamics and Viscoelastic Properties

Houari Bettahar, Quan Zhou

The mechanical interactions between particles and cell membranes are critical for understanding cellular processes such as endocytosis, cell signaling, and drug delivery. This study investigates single particle-cell dynamic interaction and the viscoelasticity properties of MiaPaCa-2 pancreatic cancer cells and fibroblasts using a …

Longitudinal Monitoring of Glioblastoma Small Extracellular Vesicle Evolution Using a Nanodiagnostic to Detect Emergence of Glioma Stem Cells Driving Recurrent Disease

Zhen Zhang, Richard J. Lobb, Paul A. Tooney, Jing Wang et autres

Abstract Assessing therapeutic response in glioblastoma (GBM) is a major factor limiting the clinical development of new and effective therapies. The intracranial location limits serial biopsies, and only provides an intermittent view of the tumor molecular profile from the initial resection. Liquid …

au, cn (code pays fourni par la source)

Glycan Profiling in Small Extracellular Vesicles with a SERS Microfluidic Biosensor Identifies Early Malignant Development in Lung Cancer (Adv. Sci. 33/2024)

Quan Zhou, X. Niu, Zhen Zhang, Kenneth J. O’Byrne et autres

Non-Small Cell Lung Cancer In article number 2401818, Alain Wuethrich, Richard J. Lobb, Matt Trau, and co-workers develop a surface-enhanced Raman scattering (SERS) assay for detecting glycan signatures in extracellular vesicles (EVs) of early-stage lung cancer patients. This cover artwork highlights the …