Jiuhai Zhao

A single‐nucleus transcriptomic atlas reveals distinct cell identities and key regulators of cellular differentiation during early rice seed development

Yingxiang Liu, Haoyu Wang, Min Xu, Ziming Qiu et autres

The early morphogenesis of the embryo and endosperm sets the upper limit of rice grain yield; yet, the cellular dynamics and regulatory mechanisms underlying this critical stage remain largely elusive. Here, we present a single-cell atlas of developing rice seeds based on …

cn (code pays fourni par la source)

Overexpression of DWARF14-LIKE2 in Arabidopsis thaliana alters multiple traits related to plant morphology and osmotic and salt stress tolerance

Xiaofan Nie, Liangliang Li, Chenbo Zhu, Kaixin Yang et autres

cn, us (code pays fourni par la source)

Small nucleolar RNA HIDDEN TREASURE 2 reduces drought tolerance via multiple pathways in Arabidopsis

Liangliang Li, Xianpeng Yang, Haodong Huang, Chenbo Zhu et autres

Small nucleolar RNAs (snoRNAs) contribute to ribosome biogenesis and modulate various aspects of plant growth and development. Given that osmotic stress downregulates numerous genes associated with ribosome biogenesis in roots, we hypothesize that snoRNAs might function in modulating plant responses to osmotic …

cn, us (code pays fourni par la source)

A Rapid and Visual Soybean Hairy Root Transformation Protocol Using the RUBY Reporter

Zhirui Zhang, Qiushi Wang, Yu Geng, Jiuhai Zhao

-mediated hairy root transformation provides a rapid platform for gene function analysis prior to stable whole-plant transformation. However, most existing hairy root transformation methods rely on tissue culture and require chemical or fluorescence-based selection, which increases experimental complexity. Here, we describe a …

cn (code pays fourni par la source)

Spatial Flavonoid Accumulation in Soybean Pericycle Restricts Phytophthora sojae Invasion

Yu Geng, Jiuhai Zhao, Q. C. Wang, Qunqing Wang

Abstract Plant root immunity depends on the coordinated activation of defenses across spatially distinct tissues, yet the cellular mechanisms underlying this organization remain poorly understood. Here, we integrate live imaging, single-cell transcriptomics, genetic manipulation, metabolomics, and spatial metabolic profiling to dissect soybean …

cn (code pays fourni par la source)

Overexpression of DRM2 Increases Resistance to Botrytis cinerea by Modulating JA Signalling Pathway in Tomato

Mannan Zhang, Hongyu Han, Yani Li, Menghan Zhang et autres

The fungal pathogen Botrytis cinerea (B. cinerea) is highly destructive and threatens tomato production and shelf life. While plant defence mechanisms against B. cinerea are well-studied, its epigenetic regulation remains largely unknown. DRM2-mediated de novo DNA methylation plays pivotal roles in plant …

cn (code pays fourni par la source)

Antagonistic systemin receptors integrate the activation and attenuation of systemic wound signaling in tomato

Ke Xu Zhou, Fangming Wu, Lei Deng, Yu Xiao et autres

Pattern recognition receptor (PRR)-mediated perception of damage-associated molecular patterns (DAMPs) triggers the first line of inducible defenses in both plants and animals. Compared with animals, plants are sessile and regularly encounter physical damage by biotic and abiotic factors. A longstanding problem concerns …

cn, us (code pays fourni par la source)

Chromosome-level genome assembly of Solanum pimpinellifolium

Hongyu Han, Xiuhong Li, Tianze Li, Qian Chen et autres

Solanum pimpinellifolium, the closest wild relative of the domesticated tomato, has high potential for use in breeding programs aimed at developing multi-pathogen resistance and quality improvement. We generated a chromosome-level genome assembly of S. pimpinellifolium LA1589, with a size of 833 Mb …

cn (code pays fourni par la source)

Stemphylium lycopersici Nep1-like Protein (NLP) Is a Key Virulence Factor in Tomato Gray Leaf Spot Disease

Jiajie Lian, Hongyu Han, Xizhan Chen, Qian Chen et autres

The fungus Stemphylium lycopersici (S. lycopersici) is an economically important plant pathogen that causes grey leaf spot disease in tomato. However, functional genomic studies in S. lycopersici are lacking, and the factors influencing its pathogenicity remain largely unknown. Here, we present the …

cn (code pays fourni par la source)

Coordinated cytokinin signaling and auxin biosynthesis mediates arsenate-induced root growth inhibition

Tianli Tu, Shuangshuang Zheng, Panrong Ren, Xianwen Meng et autres

Interactions between plant hormones and environmental signals are important for the maintenance of root growth plasticity under ever-changing environmental conditions. Here, we demonstrate that arsenate (AsV), the most prevalent form of arsenic (As) in nature, restrains elongation of the primary root through …

cn (code pays fourni par la source)

Mediator Subunit MED25 Physically Interacts with PHYTOCHROME INTERACTING FACTOR4 to Regulate Shade-Induced Hypocotyl Elongation in Tomato

Wenjing Sun, Hongyu Han, Lei Deng, Chuanlong Sun et autres

Shade triggers important adaptive responses such as the shade-avoidance syndrome, which enable plants to respond to the depletion of photosynthetically active light. The basic helix-loop-helix transcription factors PHYTOCHROME INTERACTING FACTORS (PIFs) play a key role in the shade-avoidance syndrome network by regulating …

cn (code pays fourni par la source)

Mediator Subunit MED25 Couples Alternative Splicing of JAZ Genes with Fine-Tuning of Jasmonate Signaling

Fangming Wu, Lei Deng, Qingzhe Zhai, Jiuhai Zhao et autres

JASMONATE ZIM-DOMAIN (JAZ) transcriptional repressors are key regulators of jasmonate (JA) signaling in plants.At the resting stage, the C-terminal Jas motifs of JAZ proteins bind the transcription factor MYC2 to repress JA signaling.Upon hormone elicitation, the Jas motif binds the hormone receptor …

cn (code pays fourni par la source)