Udo Kraushaar

A scalable mesh microelectrode array platform for longitudinal electrophysiology in neural spheroids

Tom Stumpp, Fulya Ersoy, Michael Mierzejewski, Meike Beer et autres

Electrophysiological interfacing remains difficult in three-dimensional in vitro models, when using planar microelectrode arrays or optical methods. This challenge limits experimental progress using such models, despite their promise of better physiological relevance than monolayer cell culture. Mesh MEAs which can integrate conformally …

us, ir (code pays fourni par la source)

Functional Expression of Nicotinic Receptors on iPSC-Derived Astrocytes and Signalling Disturbances by a Panel of Neonicotinoid Pesticides and Their Metabolites

Eike Cöllen, Chiara Wolfbeisz, Heidrun Leisner, Karin Grillberger et autres

Little is known about how nicotinic signalling in human astrocytes may contribute to the functional neurotoxicity of compounds related to tobacco alkaloids and neonicotinoid pesticides. We generated a single-cell Ca2+-imaging assay in induced pluripotent stem cell (iPSC)-derived astrocytes, and profiled functional expressions …

de, at, us (code pays fourni par la source)

NeuroTool - Humanzellbasierte Neuronennetzwerke für Wirkstofffindung, Toxikologie, und biomedizinische Forschung; Teilprojekt NMI: Assayentwicklung und Elektrophysiologie

Jasmin Kormann, Udo Kraushaar

Medikamente, Umweltgifte und pathophysiologische Prozesse können ein breites Spektrum funktioneller Störungen des Nervensystems verursachen. Die Forschung in diesem Bereich stützt sich weiterhin in erheblichem Umfang auf tierexperimentelle Ansätze. Verfügbare in-vitro-Systeme auf Basis humaner, aus Stammzellen differenzierter Neuronen eröffnen zwar neue Möglichkeiten, sind …

Towards Closed-Loop Neuromodulation for Type 2 Diabetes With Ex Vivo Validation of Beta-Cell Activity and FOPP Detection

Razieh Eskandari, Moustafa Nawito, Mostafa Katebi, Udo Kraushaar et autres

We present in this paper an implantable closed-loop neuromodulation prototype system for type 2 diabetes (T2D) management, which leverages pancreatic electrophysiology as both a sensing and therapeutic modality. Among candidate biomarkers, the fraction of plateau phase (FOPP) emerges as a robust indicator …

cn, de, us (code pays fourni par la source)

Gap Junctional Communication Required for the Establishment of Long-Term Robust Ca2+ Oscillations Across Human Neuronal Spheroids and Extended 2D Cultures

Eike Cöllen, Ayla Aksoy‐Aksel, Yaroslav Tanaskov, Kevin Wulkesch et autres

Synchronized oscillatory fluctuations in intracellular calcium concentration across extended neuronal networks represent a functional indicator of connectivity and signal coordination. In this study, a model of human immature neurons (differentiated from LUHMES precursors) has been used to establish a robust protocol for …

de (code pays fourni par la source)

Gap Junctional Communication Required for the Establishment of Long-Term Robust Ca2+ Oscillations Across Human Neuronal Spheroids and Extended 2D Cultures

Eike Cöllen, Ayla Aksoy‐Aksel, Yaroslav Tanaskov, Kevin Wulkesch et autres

Synchronized oscillatory fluctuations in intracellular calcium concentration across extended neuronal networks represent a functional indicator of connectivity and signal coordination. In this study, human dopaminergic neurons (LUHMES cell line) have been used to establish a robust protocol for generating reproducible intracellular Ca²⁺ …

de (code pays fourni par la source)

Recording of transcellular voltage deflections from cardiomyocytes on MEA devices using laser poration

Timm Danker, Udo Kraushaar, Jasmin Schäfer

de (code pays fourni par la source)

Pankreas-Implantat zur Blutzuckerüberwachung (PanaMEA); Teilprojekt NMI: Pankreas-Interface

Udo Kraushaar

Im Rahmen des vom BMBF geförderten Projekts PanaMEA wurde ein neuartiges, vollständig implantierbares Sensorsystem zur kontinuierlichen, driftfreien Überwachung des Blutzuckerspiegels bei Typ-2-Diabetes entwickelt. Das Besondere am Konzept ist die Nutzung der natürlichen elektrischen Aktivität pankreatischer Betazellen als körpereigener Biosensor – ein innovativer …

The long way from raw data to NAM-based information: Overview on data layers and processing steps

Jonathan H. Blum, Markus Brüll, Jan Georg Hengstler, Daniel Reto Dietrich et autres

Toxicological test methods generate raw data and provide instructions on how to use these to determine a final outcome such as a classification of test compounds as hits or non-hits. The data processing pipeline provided in the test method description is often …

de, it, ch, us, fr, nl, gb (code pays fourni par la source)

Compound Testing of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Using Multi-Well Microelectrode Arrays

Udo Kraushaar

de (code pays fourni par la source)

Aberrant neuronal connectivity and network activity of neurons derived from patients with idiopathic schizophrenia

Johanna Heider, Emilio Pardo González, Sophia-Marie Hartmann, Nirmal Raman Kannaiyan et autres

Schizophrenia (SCZ) is a psychiatric disorder with a strong genetic determinant. A major hypothesis to explain disease aetiology comprises synaptic dysfunction associated with excitatory-inhibitory imbalance of synaptic transmission, ultimately contributing to impaired network oscillation and cognitive deficits associated with the disease. Here, …

de (code pays fourni par la source)

P01-48 Expanding functional neurotoxicity investigations: exploring synchronized calcium-driven oscillatory activity in a human dopaminergic 3D model

Judith Schäfer, J. Schneider, Eike Cöllen, Marcel Leist et autres

de (code pays fourni par la source)