Marc Oeller

In the same cell, the proteome defines cellular state and the transcriptome marks transitions

Marvin Thielert, Enes Ugur, Maximilian Zwiebel, Marc Oeller et autres

Summary Bulk transcriptome and proteome correlate only modestly, but this has not been investigated in the same cell or across cell-state changes. Here we introduce a scalable technology that quantifies thousands of proteins and transcripts in the same cell, separating RNA from …

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Targeting specific kinase substrates rescues increased colitis severity induced by the Crohn’s disease–linked LRRK2-N2081D variant

George Heaton, Xingjian Li, Xianting Li, Xiaoting Zhou et autres

LRRK2 contains a kinase domain where the N2081D Crohn's disease (CD) risk and the G2019S Parkinson's disease (PD) pathogenic variants are located. It is not clear how the N2081D variant increases CD risk or how these adjacent mutations give rise to distinct …

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Solid-Phase Extraction Capture (SPEC) in nanoliter volumes for fast, robust and ultra-sensitive proteomics

Tim Heymann, Denys Oliinyk, Lukas T. Henneberg, Edwin H. Rodriguez et autres

Abstract Sample preparation remains a critical bottleneck in mass spectrometry (MS)-based proteomics, particularly for limited sample amounts where surface adsorption and dilution cause substantial losses. Here, we present Solid-Phase Extraction Capture (SPEC), a workflow that confines protein processing to nanoliter volumes within …

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nanoPhos enables ultra-sensitive and cell-type resolved spatial phosphoproteomics

Denys Oliinyk, Tim Heymann, Lukas T. Henneberg, Anastasiya Bardziukova et autres

Abstract Mass spectrometry (MS)-based phosphoproteomics has transformed our understanding of cell signaling, yet current workflows face limitations in sensitivity and spatial resolution at sub-microgram inputs. Here, we present nanoPhos, a robust method that extends phosphoproteomics to nanogram scale, making it compatible with …

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Deep Visual Proteomics maps proteotoxicity in a genetic liver disease

Florian A. Rosenberger, Sophia C. Mädler, Katrine Holtz Thorhauge, Sophia Steigerwald et autres

Abstract Protein misfolding diseases, including α1-antitrypsin deficiency (AATD), pose substantial health challenges, with their cellular progression still poorly understood1–3. We use spatial proteomics by mass spectrometry and machine learning to map AATD in human liver tissue. Combining Deep Visual Proteomics (DVP) with …

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BPS2025 - Quantifying and predicting antibody non-specificity in the clinical landscape: Microfluidics and machine learning

Ella de Csilléry, Hannes Ausserwӧger, Marc Oeller, Isabel Waibel et autres

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BPS2025 - Quantifying and predicting antibody non-specificity in the clinical landscape: Microfluidics and machine learning

Ella de Csilléry, Hannes Ausserwӧger, Marc Oeller, Isabel Waibel et autres

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Bridging the Gap From Proteomics Technology to Clinical Application: Highlights From the 68th Benzon Foundation Symposium

Vincent Albrecht, Thierry M. Nordmann, Andreas Mund, Lisa Schweizer et autres

The 68th Benzon Foundation Symposium brought together leading experts to explore the integration of mass spectrometry-based proteomics and artificial intelligence to revolutionize personalized medicine. This report highlights key discussions on recent technological advances in mass spectrometry-based proteomics, including improvements in sensitivity, throughput, …

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The proteomic landscape of proteotoxic stress in a fibrogenic liver disease

Florian A. Rosenberger, Sophia C. Mädler, Katrine Holtz Thorhauge, Sophia Steigerwald et autres

Abstract Protein misfolding diseases, including alpha-1 antitrypsin deficiency (AATD), pose significant health challenges, with their cellular progression still poorly understood 1–3 . We utilize spatial proteomics by mass spectrometry and machine learning to map AATD in human liver tissue. Combining Deep Visual …

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Targeting Specific Kinase Substrates Rescues Increased Colitis Severity Induced by the Crohn’s Disease-Linked LRRK2-N2081D Variant

George Heaton, Xianting Li, Xiaoting Zhou, Yuanxi Zhang et autres

Abstract LRRK2 contains a kinase domain where both the N2081D Crohn’s disease (CD) risk and the G2019S Parkinson’s disease (PD)-pathogenic variants are located. The mechanisms by which the N2081D variant increase CD risk, and how these adjacent mutations result in distinct diseases, …

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Risk willingness in multiple system atrophy and Parkinson’s disease understanding patient preferences

Alexander Bernhardt, Marc Oeller, Isabel Friedrich, Emre Kocakavuk et autres

Disease-modifying therapeutics in the α-synucleinopathies multiple system atrophy (MSA) and Parkinson's Disease (PD) are in early phases of clinical testing. Involving patients' preferences including therapy-associated risk willingness in initial stages of therapy development has been increasingly pursued in regulatory approval processes. In …

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OS-041-YI A spatiotemporal map of the hepatocyte proteome in alpha-1 antitrypsin deficiency by single-cell and visual proteomics

Florian A. Rosenberger, Katrine Holtz Thorhauge, Sophia C. Mädler, Malin Fromme et autres