Tirtha Das Banerjee

(csv_files) A spatial mRNA profiling workflow using Rapid Amplified Multiplex FISH (RAMFISH)

Tirtha Das Banerjee

(csv_files) A spatial mRNA profiling workflow using Rapid Amplified Multiplex FISH (RAMFISH)

Tirtha Das Banerjee

Parallels in leg and wing proximal-distal patterning in Holometabola

Jeriel Lee, Tirtha Das Banerjee, Antonia Monteiro

ABSTRACT The wings and legs of insects are both appendages that develop along a proximal-distal (PD) axis and likely share many underlying patterning mechanisms. Comparisons between the two appendages have been detailed in Drosophila melanogaster , a derived insect where both traits …

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Modular fluidic automation platform with integrated thermal control for multi-step molecular imaging workflows

Tirtha Das Banerjee, Joshua Raine, Ajay Sriram Mathuru, Antónia Monteiro

Abstract Automation of multi-step mRNA imaging protocols increases reproducibility and throughput in spatial biology, as many workflows require repeated buffer exchanges, precise timing, and controlled reaction conditions. Commercial automation platforms can be expensive, proprietary, and difficult to customise, limiting their use in …

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Disruption of chrna5 blunts aversion and adaptive transcriptomic responses to nicotine and alcohol

Tanisha Goel, Joshua Raine, Caroline Kibat, Jeff Winxin Collado et autres

mutants also exhibited drug-independent changes in appetite and circadian rhythms. We expect these results to give new insights into genetic predisposition that modulates vulnerability to nicotine and alcohol abuse.

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Shared regulatory networks link vein positioning and eyespot ring formation in butterflies

Tirtha Das Banerjee, Antónia Monteiro

Novel traits might evolve via the repurposing of existing gene-regulatory networks. To demonstrate that a novel trait is the product of such co-option, we sought to show that the same genes and genetic interactions are taking place in different traits in a …

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A novel Hox gene promoter fuels the evolution of adaptive phenotypic plasticity in wing eyespots of satyrid butterflies

Shen Tian, Bonnie H. Lee, Tirtha Das Banerjee, Suriya Narayanan Murugesan et autres

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chrna3 Modulates Alcohol Response

Joshua Raine, Caroline Kibat, Tirtha Das Banerjee, Antónia Monteiro et autres

Alcohol use disorders (AUDs) are complex phenomena governed by genetics, neurophysiology, environment, and societal structures. New methods to understand the underlying neurogenetics are valuable for designing personalized interventional strategies. Here, we used a two-choice self-administration zebrafish assay to isolate the function of …

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Role of chrna5 in multi-substance preference and phenotypes comorbid with the development of substance dependence

Tanisha Goel, Joshua Raine, Caroline Kibat, Jeff Winxin Collado et autres

Abstract Addiction to nicotine and alcohol continues to be a leading cause of death and loss of productivity as measured in disability-adjusted life years. Polymorphisms in the nicotinic acetylcholine receptor subunit α5 (CHRNA5) have been identified as risk factors associated with nicotine …

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A novel Hox gene promoter fuels the evolution of adaptive phenotypic plasticity

Shen Tian, Bonnie H. Lee, Tirtha Das Banerjee, Suriya Narayanan Murugesan et autres

Abstract Adaptive phenotypic plasticity allows organisms to display distinct phenotypes in response to variable environments, but little is known about the genomic changes that promote the evolution of plasticity on a macroevolutionary scale. Combining spatial transcriptomics, comparative genomics, and genome editing, we …

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Mapping Gene Expression in Whole Larval Brains of Bicyclus anynana Butterflies

Tirtha Das Banerjee, Linwan Zhang, Antónia Monteiro

Butterfly larvae display intricate cognitive capacities and behaviors, but relatively little is known about how those behaviors alter their brains at the molecular level. Here, we optimized a hybridization chain reaction 3.0 (HCR v3.0) protocol to visualize the expression of multiple RNA …

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Spatial mRNA Profiling Using Rapid Amplified Multiplexed-FISH (RAM-FISH)

Tirtha Das Banerjee, Joshua Raine, Ajay Sriram Mathuru, Kok Hao Chen et autres

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